Total-nutrient kelp oligosaccharide biological synergistic fertilizer and preparation method thereof

A technology of biological synergy and kelp oligosaccharides, applied in fertilization devices, fertilizer mixtures, applications, etc., can solve the problems of lack of degradation, weakening the synergistic effect of seaweed fertilizer, and not reflecting the full nutrition of kelp

Inactive Publication Date: 2012-07-18
青岛丰泰海洋生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] (2) Alginic acid in alginic acid-containing fertilizers does not reach the 2% level of my country's industry standard, and the phenomenon of compounding alginic acid, nitrogen, phosphorus, potassium, trace elements, fulvic acid and humic acid is serious, which also reduces the amount of algae The active ingredients of other seaweeds in the fertilizer weaken the synergistic effect of the seaweed fertilizer
[0007] (3) Lack of a specific lyase that degrades macromolecular alginate in kelp into oligosaccharides, which is also a technical bottleneck restricting kelp bio-fermentation fertilizers. The market kelp fermentation fertilizers basically use cellulase, protease, yeast, Fermentation of papain, hemicellulase and pectinase, etc. These enzymes cannot degrade the most active alginic acid metal salt with a valence of more than 2 in kelp into small molecules of water-soluble fucoidan oligosaccharides, which are dissolved from seaweed
[0008] (4) In the production of existing seaweed fertilizer, the soaking water of sea-tangle is not utilized, but is discharged as waste water, which not only wastes water-soluble active ingredients in sea-tangle such as iodine, potassium, magnesium, manganese, titanium, trace elements, Vitamins, N-containing compounds, highly unsaturated fatty acids, seaweed enzymes, betaine, amino acids and various natural plant growth regulators (plant auxins, gibberellins, cytokinins, polyphenolic compounds and natural antibiotic substances) and Fucoidan sulfate and mannitol also pollute the environment and cannot reflect the full nutrition of kelp

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Weigh 100Kg of kelp, add 2000Kg of tap water, soak for 4 hours, remove the kelp, cut it into 10×20cm blocks, grind it into a particle size of less than 1mm with a colloid mill, and put it into a fermenter; After filtering, the filtrate is pumped into a fermenter. Add 40 kg of alginic acid lyase with an enzyme activity of 88u / ml to the fermenter, ferment at 45°C for 72 hours, the enzymolyzed liquid appears watery, and monitor the alginic acid content in the enzymatically hydrolyzed liquid by the 9-nitrogen-miscellaneous method , controlled between 0.6-1.0, and then filtered with a plate frame, the filtrate was concentrated at a low temperature at 48-50°C, the concentrated specific gravity was controlled at 1.18-1.20, and sterilized to obtain 350 kg of full-nutrient kelp oligosaccharide bioaugmentation liquid fertilizer. The synergistic fertilizer of the present invention contains all the nutrients of kelp, including alginic acid oligosaccharide: 4.2%, mannitol: 1.5%, fu...

Embodiment 2

[0021] During the specific operation, weigh 100Kg kelp, add 2000Kg tap water, soak for 4 hours, remove the kelp, cut it into 10×20cm blocks, and then grind it into a particle size less than 1mm with a colloid mill, put it into the fermentation tank; soak the kelp Water waste. Add 400 kg of water and 40 kg of alginic acid lyase with an enzyme activity of 88u / ml to the fermenter, ferment at 45°C for 72 hours, the enzymatic hydrolyzate appears watery, and monitor the enzymatic hydrolyzate with the 9-nitrogen-purple method The alginic acid content in the medium is controlled between 0.6-1.0, and then filtered with a plate frame, and the filtrate is concentrated at a low temperature at 48-50°C, the concentrated specific gravity is controlled at 1.18-1.20, and the alginic acid oligosaccharide is controlled at 4.2%. Bacteria, obtain 320 kilograms of laminar oligosaccharide liquid fertilizers. The nutrients in the fertilizer are alginic acid oligosaccharide: 4.2%, mannitol: 0.21%, fu...

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PUM

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Abstract

The invention relates to a total-nutrient kelp oligosaccharide biological synergistic fertilizer and a preparation method thereof. The preparation method comprises the steps of: firstly soaking kelps in water and filtering soaking water; crushing the soaked kelps, adding kelp powder into the filtered soaking water and adding alginic acid lyase for enzymolysis; and after the enzymolysis is completed, conducting low-temperature concentration to enzymatic hydrolyzate to complete the preparation of liquid fertilizer. Since the temperature in the whole production process is controlled to be below 50 DEG C and any chemical is not used, the total-nutrient kelp oligosaccharide biological synergistic fertilizer has the characteristics of environmental friendliness, ecological and environmental protection, energy saving and emission reduction, natural plant growth promoting factors in the kelps are fully reserved, compositions such as iodine, alginic acid oligosaccharide, mannitol and fucoidan capable of resisting diseases and adversity, preserving water and moisture and resisting ionizing radiation, ultraviolet radiation and nuclear radiation are added and the efficacy that medicine and the fertilizer are combined together is obtained.

Description

technical field [0001] The invention belongs to the technical field of seaweed biological fertilizers, and in particular relates to a full-nutrition kelp oligosaccharide biosynergistic fertilizer and a preparation method thereof. technical background [0002] Kelp is a huge adsorbent, it can concentrate 440,000 times the nutrients in the ocean, which is very suitable for feeding barren land. In addition, a large amount of nutrients in the land are discharged into the ocean through rainwater and rivers to form a reasonable biological cycle. Worldwide, about 21.5 million tons of nitrogen and 2 million tons of potassium flow from land to rivers through rainwater and then into the ocean. Kelp as an agricultural plant growth regulator is to recombine the nutrients that originally came from land through the "reactor" of kelp and return them to land plants. [0003] Kelp bio-fertilizer is a kind of agricultural seaweed bio-fertilizer that can promote crop growth, increase yield, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00
Inventor 许加超许帆盛泰赵瑜刘存海冯志君高昕付晓婷
Owner 青岛丰泰海洋生物科技有限公司
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