Total-nutrient kelp oligosaccharide biological synergistic fertilizer and preparation method thereof
A technology of biological synergy and kelp oligosaccharides, applied in fertilization devices, fertilizer mixtures, applications, etc., can solve the problems of lack of degradation, weakening the synergistic effect of seaweed fertilizer, and not reflecting the full nutrition of kelp
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Embodiment 1
[0019] Weigh 100Kg of kelp, add 2000Kg of tap water, soak for 4 hours, remove the kelp, cut it into 10×20cm blocks, grind it into a particle size of less than 1mm with a colloid mill, and put it into a fermenter; After filtering, the filtrate is pumped into a fermenter. Add 40 kg of alginic acid lyase with an enzyme activity of 88u / ml to the fermenter, ferment at 45°C for 72 hours, the enzymolyzed liquid appears watery, and monitor the alginic acid content in the enzymatically hydrolyzed liquid by the 9-nitrogen-miscellaneous method , controlled between 0.6-1.0, and then filtered with a plate frame, the filtrate was concentrated at a low temperature at 48-50°C, the concentrated specific gravity was controlled at 1.18-1.20, and sterilized to obtain 350 kg of full-nutrient kelp oligosaccharide bioaugmentation liquid fertilizer. The synergistic fertilizer of the present invention contains all the nutrients of kelp, including alginic acid oligosaccharide: 4.2%, mannitol: 1.5%, fu...
Embodiment 2
[0021] During the specific operation, weigh 100Kg kelp, add 2000Kg tap water, soak for 4 hours, remove the kelp, cut it into 10×20cm blocks, and then grind it into a particle size less than 1mm with a colloid mill, put it into the fermentation tank; soak the kelp Water waste. Add 400 kg of water and 40 kg of alginic acid lyase with an enzyme activity of 88u / ml to the fermenter, ferment at 45°C for 72 hours, the enzymatic hydrolyzate appears watery, and monitor the enzymatic hydrolyzate with the 9-nitrogen-purple method The alginic acid content in the medium is controlled between 0.6-1.0, and then filtered with a plate frame, and the filtrate is concentrated at a low temperature at 48-50°C, the concentrated specific gravity is controlled at 1.18-1.20, and the alginic acid oligosaccharide is controlled at 4.2%. Bacteria, obtain 320 kilograms of laminar oligosaccharide liquid fertilizers. The nutrients in the fertilizer are alginic acid oligosaccharide: 4.2%, mannitol: 0.21%, fu...
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