Strain for producing glutamic acid decarboxylase and method for producing gamma-aminobutyric acid by utilizing strain
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A technology of aminobutyric acid and sodium glutamate, applied in the field of food biology, can solve the problems of severe reaction conditions, high toxicity, expensive raw materials, etc., and achieve the effect of efficient production
Active Publication Date: 2013-09-25
健隆生物科技股份有限公司
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In contrast, the chemical synthesis method has a long history and rapid reaction, but the raw materials used are expensive, highly toxic, the reaction condi
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Embodiment 1
[0030] Embodiment 1 Lactobacillus plantarum ( Lactobacillus plantarum ) Fermentation culture of SK30.001 strain
[0031] The above strains were cultured on medium A at 30°C for 24 hours. The culture was inoculated in seed medium B containing 100 mL, cultured at 30°C for 24 hours, then transferred to fermentation medium C, with an inoculum size of 1%-10%, and fermented at 30°C for 24 hours.
[0032] Medium A: peptone 11 g / L, beef extract 20 g / L, yeast 10 g / L, diammonium hydrogen citrate 1g / L, glucose 20g / L, Tween 80 1ml / L, sodium acetate 1g / L, Dipotassium hydrogen phosphate 2 g / L, magnesium sulfate 0.7g / L, manganese sulfate 0.7g / L, agar 2%;
[0033] Seed medium B: peptone 11 g / L, beef extract 20 g / L, yeast 10 g / L, diammonium hydrogen citrate 1 g / L, glucose 20 g / L, Tween 80 1 mL / L, sodium acetate 1 g / L , dipotassium hydrogen phosphate 2 g / L, magnesium sulfate 0.7g / L, manganese sulfate 0.7g / L, prepared with deionized water;
[0034] Fermentation medium C: peptone 15 g / L, beef...
Embodiment 2
[0036] The new γ-aminobutyric acid producing bacteria were sent to the China Type Culture Collection Center to determine the morphological characteristics, physiological and biochemical characteristics of the bacteria and 16sRNA gene sequence analysis, and were identified as Lactobacillus plantarum ( Lactobacillus plantarum ) SK30.001.
Embodiment 3
[0037] Example 3 Cell transformation to produce γ-aminobutyric acid
[0038] Centrifugation: Centrifuge the fermented cells at 2000g for 5 min.
[0039] Transformation conditions: the bacterial cells were suspended in a buffer solution with a sodium glutamate content of 8 g / L and a pH of 4.0, and transformed at a temperature of 30° C. for 36 hours to obtain a transformation liquid.
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Abstract
The invention relates to a strain for producing glutamic acid decarboxylase and a method for producing gamma-aminobutyric acid by utilizing the strain, belonging to the field of food biotechnology. The invention discloses lactobacillus plantarum SK30.001. The lactobacillus plantarum SK30.001 is screened from pickled vegetables and is preserved in China Center for Type Culture Collection (CCTCC), and the preserve number is CCTCC NO: M2013250. The invention further provides a method for producing the gamma-aminobutyric acid through fermentation by utilizing the strain. The method comprises the following steps of: (a) culturing the strain in a fermentation culture medium, so as to obtain lactobacillus plantarum thallus; (b) converting sodium glutamate by utilizing the lactobacillus plantarum thallus to generate the gamma-aminobutyric acid; and (c) carrying out separation and purification of the gamma-aminobutyric acid. The gamma-aminobutyric acid produced by utilizing method is safe and reliable, is a functional product with a good market potential and is widely applied to industries of foods, cosmetics, medicines and the like. According to the strain for producing the glutamic acid decarboxylase and the method for producing the gamma-aminobutyric acid by utilizing the strain, the gamma-aminobutyric acid can be efficiently produced, the mass production is realized easily, and a novel method for industrial preparation of the gamma-aminobutyric acid is provided.
Description
technical field [0001] The present invention relates to a kind of Lactobacillus plantarum capable of producing glutamic acid decarboxylase ( Lactobacillus plantarum ) SK30.001 screening and a method for producing gamma-aminobutyric acid through fermentative transformation using the strain belong to the field of food biotechnology. Background technique [0002] γ-aminobutyric acid (GABA for short) is an important inhibitory neurotransmitter, which has important physiological regulation effects on mammals, such as lowering blood pressure, improving sleep and anti-arrhythmia. In addition, GABA has been found to regulate the reproductive system in recent years. Therefore, the market demand for GABA is increasing day by day, but its natural content is very low, and relying solely on the extraction of natural substances is far from meeting the market demand. [0003] The current methods for producing GABA mainly include chemical synthesis and biosynthesis. Biosynthesis is furth...
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