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Disk type laccase sensor electrode and preparation method thereof

A sensor electrode and disk electrode technology, applied in the field of disk type laccase sensor electrode and its preparation, can solve the problems of poor stability and low sensitivity of laccase sensor, and achieve low detection limit, wide linear range and wide linear range Effect

Inactive Publication Date: 2013-09-25
TAIYUAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the characteristics of high electrical conductivity, large specific surface area, strong adsorption capacity and the ability to firmly adsorb biological macromolecules such as enzymes combined with the good film-forming properties and biocompatibility of chitosan overcome the low sensitivity of laccase sensors. , the problem of poor stability

Method used

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  • Disk type laccase sensor electrode and preparation method thereof
  • Disk type laccase sensor electrode and preparation method thereof
  • Disk type laccase sensor electrode and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 0.2 g of chitosan with a molecular weight of 50,000 was dissolved in 50 ml of acetic acid solution with a mass concentration of 0.5%, and ultrasonically oscillated for 60 min. Then 20ml of AgNO with a mass concentration of 5mg / ml 3 Add it into the above-mentioned chitosan solution, stir it magnetically for 60 minutes, add 15 ml of sodium citrate solution with a mass concentration of 2% dropwise, adjust the pH value of the solution to 6 with 0.1mol / L NaOH, and stir at room temperature for 20 hours to obtain chitosan Sugar-silver nanoparticle sol.

[0032] Pipette 50 μL of the above chitosan-silver nanoparticle sol and add it to 10 ml of laccase solution with a mass concentration of 5 mg / ml, and stir magnetically in an ice bath at 4°C for 48 hours to fully immobilize the laccase on the chitosan-silver nanoparticle solution. In the particle colloid, a laccase-loaded chitosan-silver nanoparticle sol is obtained.

[0033] A 316L stainless steel disc electrode with a diamet...

Embodiment 2

[0037] The operating process and experimental conditions of Example 2 are the same as in Example 1, except that the quality of chitosan is changed to 1 g, and the volume of the chitosan-silver nanoparticle sol loaded with laccase is changed to 20 μL. The mass concentration of p-benzoquinone becomes 20mM, and the electrochemical reduction potential of benzoquinone becomes -0.1V.

[0038] Using the above-mentioned three-electrode working system, measure the response current of different concentrations of hydroquinone on the modified electrode, and make a response current-concentration curve. figure 2 The results showed that the constructed sensor had a sensitivity of 0.31A / M, a linear monitoring range of 0.1-10μM, and a detection limit of 0.29μM.

Embodiment 3

[0040] The operating process and experimental conditions of embodiment 3 are the same as embodiment 1, except that 20ml of AgNO with a mass concentration of 5mg / ml3 Change to 5 mL of HAuCl with a mass concentration of 0.1% 4· 4H 2 O, change the mass concentration of sodium citrate to 0.5%.

[0041] Using the above-mentioned three-electrode working system, measure the response current of different concentrations of hydroquinone on the modified electrode, and make a response current-concentration curve. image 3 The results showed that the constructed sensor had a sensitivity of 0.42A / M, a linear monitoring range of 0.05-15μM, and a detection limit of 0.22μM.

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Abstract

The invention relates to a disk type laccase sensor electrode and a preparation method thereof, which is characterized in that the electrode is composed of a stainless steel disk electrode sputtered with gold film and a chitosan-metal nanoparticle sensitive membrane loading laccase, and the thickness of the sensitive membrane is 5-10mum. The preparation process of the electrode comprises the following steps of: preparing a chitosan-metal nanoparticle sol by an in-situ reduction method, and placing the sol into a laccase solution for immobilizing the laccase; polishing a 360L stainless steel disk electrode sputtered with the gold film on chamois leather, after repeatedly flushing with secondary distilled water, treating with an ultrasonic wave and drying, activating 20 loops by electrochemical cyclic voltammetry sweep in a PBS solution with pH being 5.0, then placing the stainless steel disk electrode into a solution containing p-benzoquinone and chitosan-metal nanoparticles loading laccase, and preparing the chitosan-metal nanoparticle sensitive membrane on a surface of the stainless steel electrode by a benzoquinone electrochemical reduction, induction and deposition method, to obtain the laccase sensor electrode. The electrode has good electric catalytic oxidation activity on phenols, and the established disk type laccase sensor can be used for detecting different phenols in an environment, and has advantages of high response sensitivity, wide linearity range and low detection limit.

Description

technical field [0001] The invention relates to a disc type laccase sensor electrode and a preparation method thereof. Background technique [0002] Phenolic substances are by-products of industries such as pesticides, spices, medicines, dyes, and polymers. They are highly toxic and extremely harmful to the environment and human body. Electrochemical laccase sensors can detect phenols in wastewater conveniently and quickly, so the construction of high-performance laccase sensors is of great significance in environmental monitoring and detection. [0003] The carrier material and immobilization method used to immobilize laccase directly determine the stability, sensitivity and detection limit of the laccase sensor. Chitosan is widely used to immobilize enzymes due to its good biocompatibility. However, the low conductivity and weak adsorption capacity of chitosan lead to low sensitivity of the immobilized enzyme electrode. Immobilized enzyme methods usually include solvent...

Claims

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Application Information

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IPC IPC(8): G01N27/327
Inventor 郭美卿陈静黄棣王晓君
Owner TAIYUAN UNIV OF TECH
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