Human acid fibroblast growth factor fusion protein, and coding gene and applications thereof

A technology for fibroblasts and fusion proteins, applied in human acidic fibroblast growth factor fusion proteins and their coding genes and application fields
CN103333256AActive Publication Date: 2013-10-02THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
3 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
Publication Date
2013-10-02

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides a fusion protein. The fusion protein comprises suppressors of gene silencing, 2A polypeptide and a human acid fibroblast growth factor protein. All or part of the human acid fibroblast growth factor protein is a natural human acid fibroblast growth factor protein; or the human acid fibroblast growth factor protein is a fusion protein formed by co-expression or splicing of the natural human acid fibroblast growth factor protein with another fibroblast factor protein, or with another protein which possesses completely different biological functions. The invention also provides the coding gene of the fusion protein, and a preparation method of genetically modified radix salviae miltiorrhizae which is used for high-efficiency expression of the human acid fibroblast factor. The genetically modified radix salviae miltiorrhizae can be used for effective treatment of operative incisions, nerve injuries, body mechanical injuries, diabetic foot, anabrosis, burned and scalded injuries, and the like, and is also applied in developing a new generation of recombinant human acidic fibroblast factor drugs.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the field of genetic engineering, in particular to a human acidic fibroblast growth factor fusion protein and its coding gene and application. Background technique

[0002] Human fibroblast growth factor belongs to a large protein family, at least 23 members have been discovered so far. Among them, acidic fibroblast growth factor (aFGF) was first isolated from bovine brain by Thomas et al. in 1984. Because its isoelectric point is 5-7, it is acidic, so it is named .

[0003] Human acidic fibroblast growth factor (haFGF) is mainly distributed in the kidney and brain tissue. It is a cytoplasmic protein that lacks an N-terminal signal peptide structure. It acts on surrounding cells mainly through autocrine and paracrine. . The haFGF polypeptide consists of 154 amino acid residues and has a molecular weight of about 16kDa. It is a complete β-sheet protein without disulfide bonds, and its secondary structure is a clover structure...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More