Dynamic-static positive-negative pressure loading experiment system and method for in-vitro cells

Abstract

The invention relates to a dynamic-static positive-negative pressure loading experiment system and method for in-vitro cells. The system comprises a gas-source gas storage bottle, a pressure reduction valve, an electromagnetic gas valve, a filter, a diverter, a high-voltage buffering container, a normal-voltage buffering container, a high-voltage container, a low-voltage container, a vacuum pump, a compression pump, a pressure culture box, a digital display temperature transducer, a digital display pressure meter strap transducer, a data acquisition system, a driving control system, a heater and a water bath tank, wherein the gas-source gas storage bottle is connected with the pressure culture box through the pressure reduction valve, the electromagnetic gas valve, the filter and the pressure container; the pressure culture box is connected with the vacuum pump and the compression pump through the pressure container; and the pressure culture box is also connected with the data acquisition system and the driving control system through a pressure sensor and a temperature sensor. The dynamic and static positive-negative pressure loading experiment system can simulate an in-vivo mechanical environment of multiple cells in vitro, in particular a pressure environment of a temporal jaw joint cavity and supply a research platform to in-vitro research on a biomechanical response of in-vivo cells.

Description

technical field [0001] The invention relates to an in vitro cell dynamic and static positive-negative pressure loading experiment system and a method thereof, belonging to the fields of cell biomechanics and tissue engineering. Background technique [0002] Cell biomechanics is a science that studies the interaction between cells and mechanical factors, and is an important part of tissue engineering. Organisms are always under the action of various mechanical forces, and tissues and cells in the body are often affected by different forms of pressure, shear force, stretching force, compression force, etc., and a mechanical system is formed under different mechanical actions. It plays an important role in a variety of activities such as proliferation, differentiation, adhesion, migration, and apoptosis. However, numerous mechanical factors make it difficult to distinguish single or combined effects in vivo. Therefore, the study of the biomechanical effect of a single mechani...

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Problems solved by technology

In China, different types of cell pressure loading experimental devices have been developed in this field, but these experimental devices still have limitations to varying degrees

Specifically include: (1) Although the existing experimental device can load static pressure and dynamic pressure, it has not yet simulated dynamic negative pressure and dynamic positive-negative pressure, which is difficult to meet the needs of temporomandibular joint research

(2) The existing experimental equipment cannot regulate the pressure loading environment of the cells, and it is difficult to meet the long-term pressure loading experiments on the cells

(3) The pressurization mode of the existing experimental device is relatively single, and the range of pressure loading is narrow, which is difficult to meet the research of cells in different pressure environments in the body

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