Rice smut resistance gene-associated disease-resistant homologous sequence and its molecular marker acquisition method

Abstract

The invention discloses a method for acquiring anti-false smut gene associated resistance gene analogs and molecular markers thereof. The method comprises steps of: designing RGA primers for complete genome resistance gene analogs to obtain RGA genotypes of rice germplasms, acquiring 137 anti-false smut phenotypes through a false-smut pathogen microinjection method, combining the RGA genotypes and the anti-false smut phenotypes to perform molecular character association analysis and to perform clone sequencing on PCR products, thus obtaining the resistance gene analogs associated with the anti-false smut gene and the molecular markers thereof. According to the invention, by means of genetic transformation and hybridization, the anti-false smut gene associated resistance gene analogs can be introduced into common cultivated rice varieties to improve resistance of the rice varieties to false smut. The anti-false smut gene associated molecular markers can be used for detecting the existence of the anti-false smut genes, indirectly selecting disease-resistant plants, and protecting identification of inoculated false smut pathogens from being influenced by climate, geographical positions and other uncertain factors, and therefore the anti-false smut gene associated molecular markers can be used for selecting early generation, shortening anti-false smut variety breeding periods, and improving breeding efficiency.

Description

technical field [0001] The invention relates to the field of plant genetic engineering of plant disease-resistant genes and molecular marker-assisted breeding, in particular to a disease-resistant gene homologous sequence of a rice smut-resistant gene and its molecular marker, and its special primers for use in rice smut-resistant rice Applications. Background technique [0002] Rice false smut is a disease at the earing stage of rice, which occurs in major rice regions in the world, and increases year by year, seriously endangering the yield and quality of rice; and the pathogen contains toxic substances and pathogenic pigments for humans, livestock, and poultry. cause direct and indirect harm to people. Since the infection period of rice false smut is mainly from rice booting to flowering stage, while the onset period is in the grain filling stage, so the infection and onset of the disease are not in the same period, which often causes artificial chemical control to lag b...

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Problems solved by technology

However, the markers used in these rice false smut resistance QTLs are microsatellite (SSR) markers, not direct markers of resistance loci, and there have been no relevant reports on their use in breeding practice

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