Microchip analysis system combining non-aqueous electrophoresis with peroxyoxalate ester chemiluminiscence

Abstract

The invention relates to a microchip analysis system combining non-aqueous electrophoresis with peroxyoxalate ester chemiluminiscence. The traditional analysis system has various problems. The microchip analysis system comprises a micro-flow control chip, a negative pressure device, a direct current power supply and a photomultiplier, wherein the micro-flow control chip comprises a substrate, a buffer liquid storage tank, a sample liquid storage tank, a sample waste liquid tank, a buffer waste liquid tank, a peroxyoxalate ester liquid storage tank, a hydrogen peroxide liquid storage tank, and a waste liquid tank arranged on the substrate; the negative pressure device comprises a vacuum flask, a miniature vacuum pump, an electric contact vacuum meter, a needle valve, a U-shaped tube pressure meter, a two-position three-way electromagnetic valve and a time relay; an output end of the direct current power supply is connected with the buffer liquid storage tank; the grounding end is connected with the buffer waste liquid tank; and the photomultiplier is directly arranged below the detection channel. The microchip analysis system is simple in structure, convenient to operate, good in compatibility, high in separation efficiency, and high in detection sensitivity; and non-aqueous electrophoresis and peroxyoxalate ester chemiluminiscence detection are not interfered by each other.

Description

technical field [0001] The invention belongs to the technical field of analysis, and relates to a micro total analysis system, in particular to a portable microchip analysis system integrating non-water chip capillary electrophoresis separation and peroxyoxalate chemiluminescence. Background technique [0002] Since Manz et al. proposed the micro-total analysis system in 1990, microchip analysis technology has opened up broad application prospects in the fields of chemistry, life science, environmental science and food science. Because chip capillary electrophoresis has the advantages of high separation efficiency, short analysis time (several seconds to tens of seconds), low consumption of reagents and samples (upgraded from microliters to piconas), high miniaturization and integration, etc., it is the most advanced microchip analysis system. commonly used separation techniques. However, low sample volume poses a challenge for highly sensitive detection. Chemiluminescence...

AI Technical Summary

Problems solved by technology

However, when the system was applied to the chemiluminescent detection of peroxyoxalate, it was found that there was an obvious laminar flow phenomenon at the junction of peroxyoxalate and hydrogen peroxide solution, although the interface disappeared quickly after passing through the spiral channel. , but as the reaction progressed, it was found that the flow rate of peroxyoxalate and hydrogen peroxide was getting slower and slower, which was not conducive to the reaction

This may be that the sample is driven by an electric field to the confluence, and it reacts with the intermediate of the reaction between peroxyoxalate and hydrogen peroxide at the junction of the two phases (on the interface between peroxyoxalate and hydrogen peroxide). The product is not conducive to the smooth flow out to the waste liquid pool W at the interface, and it accumulates more and more, which affects the flow of liquid

In addition, if the ground electrode is placed in the peroxalate pool, after a period of time, black deposits will appear, further hindering the flow of liquid

[0005] For these reasons, there is no commercialized instrument integrating chip capillary electrophoresis separation and peroxyoxalate chemiluminescence detection, let alone a commercial instrument integrating non-aqueous chip capillary electrophoresis separation and peroxyoxalate chemiluminescence detection

Images