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Method for adsorbing and purifying oil tea polyphenols by adopting macroporous resin

A technology of macroporous resin and camellia oleifera is applied in the field of purification and separation of camellia oleifera polyphenols to achieve the effects of improving utilization rate, simple process and no solvent pollution

Inactive Publication Date: 2013-11-20
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patents CN101961427A and CN102028042A respectively introduced the method of extracting and separating Camellia oleifera polyphenols from Camellia oleifera cake using solvents, but there is no patent on using resin adsorption to purify Camellia oleifera polyphenols

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Weigh 100 g of deoiled Camellia oleifera seeds, crush them to 60 mesh, extract with 1000 g of 60% ethanol aqueous solution, and remove the solvent by rotary evaporation to obtain 38.1 g of crude tea polyphenols extract in powder form. According to GB / T8313-2008, the obtained crude tea polyphenols extract was detected and analyzed with an ultraviolet spectrophotometer, and the polyphenol content of camellia oleifera was 5.92%.

[0013] Dissolve 38.1 g of the crude tea polyphenol extract obtained in 80 mL of water to prepare a 0.4 mg / mL aqueous solution of the crude tea polyphenol extract, adjust the pH value to 4.2 with 0.1 mol / L HCl, and use D101-1 type resin to adsorb it. The resin was soaked in ethanol for 24 hours before use and washed with deionized water until there was no obvious alcohol smell. The column diameter of the adsorption column is 26 mm, the column height is 143 mm, and 30 g of D101-1 resin is loaded. The aqueous solution of the crude tea polyphenols e...

Embodiment 2

[0015] Weigh 200 g of deoiled Camellia oleifera seeds, grind them to 40 mesh, extract with 4000 g of 60% ethanol aqueous solution, and remove the solvent by rotary evaporation to obtain 62.5 g of powdered crude tea polyphenols extract. According to GB / T8313-2008, the obtained crude tea polyphenols extract was detected and analyzed with an ultraviolet spectrophotometer, and the polyphenol content of camellia oleifera was 6.62%.

[0016] Dissolve 62.5 g of the crude tea polyphenol extract obtained in 100 mL of water to prepare a 0.5 mg / mL aqueous solution of the crude tea polyphenol extract, adjust the pH value to 2.3 with 0.1 mol / L HCl, and use D101-1 type resin to adsorb it. The resin was soaked in ethanol for 24 hours before use and washed with deionized water until there was no obvious alcohol smell. The column diameter of the adsorption column is 26 mm, the column height is 143 mm, and 40 g of D101-1 resin is loaded. The aqueous solution of the crude extract of camellia ol...

Embodiment 3

[0018] Weigh 100 g of Camellia oleifera husk, crush it to 80 mesh, extract with 3000 g of 80% ethanol aqueous solution, and remove the solvent by rotary evaporation to obtain 28.2 g of crude tea polyphenol extract in powder form. According to GB / T8313-2008, the obtained crude tea polyphenols extract was detected and analyzed by ultraviolet spectrophotometer, and the polyphenol content of camellia oleifera was 28.76%.

[0019] Dissolve 28.2 g of the crude tea polyphenol extract obtained in 150 mL of water to prepare a 0.6 mg / mL aqueous solution of the crude tea polyphenol extract, adjust the pH value to 3.0 with 0.1 mol / L HCl, and use D101-1 type resin to adsorb it. The resin was soaked in ethanol for 24 hours before use and washed with deionized water until there was no obvious alcohol smell. The column diameter of the adsorption column is 26 mm, the column height is 143 mm, and 30 g of D101-1 resin is loaded. The aqueous solution of the crude tea polyphenols extract flows fr...

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Abstract

The invention discloses a method for adsorbing and purifying oil tea polyphenols by adopting macroporous resin, belonging to the technical field of purification and separation of the oil tea polyphenols. The method comprises the following processes of: taking oil tea seeds and oil tea fruit shells after deoiling as raw materials for extracting the oil tea polyphenols, drying, then crushing, adopting an organic solvent for extracting to obtain a crude extract of the oil tea polyphenols, dissolving the crude extract of the oil tea polyphenols in water to prepare a water solution of the crude extract of the oil tea polyphenols, performing macroporous resin adsorption and desorption on the water solution of the crude extract of the oil tea polyphenols, and then performing rotary evaporation on a desorption solution to obtain a product of the oil tea polyphenols. According to the method disclosed by the invention, the oil tea polyphenols are obtained by adopting the macroporous resin to perform separation and purification, the processes are relatively simple, the using amount of the solvent is small, the obtained oil tea polyphenols have high purity, and the mass content can be as high as above 78%.

Description

technical field [0001] The invention relates to a method for adsorbing and purifying camellia oleifera polyphenols by using a macroporous resin, and belongs to the technical field of purification and separation of camellia oleifera polyphenols. Background technique [0002] Camellia oleifera is a plant of the family Theaceae, and is known as the world's four major woody oil plants together with olive oil, coconut, and oil palm. The tea oil extracted from camellia oleifera seeds has an unsaturated fatty acid content of more than 90%, and has the reputation of "Oriental olive oil". Camellia oleifera cake after oil extraction contains various active substances such as saponin, polyphenol, squalene, polysaccharide and protein, so camellia oleifera has high comprehensive utilization value. [0003] Camellia oleifera husk and camellia seed both contain camellia oleifera polyphenols, and the content in the husk is relatively high. Studies have shown that Camellia oleifera polyph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/82A23L1/29A61K8/97A23L33/00
Inventor 唐韶坤白杨伊文峰
Owner TIANJIN UNIV
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