Method for extracting transparent low-acyl gellan gum
A low-acyl gellan gum and extraction method technology, applied in the field of microorganisms, can solve the problems of low purity of gellan gum, environmental pollution, waste generation, etc., and achieve the effects of increasing economic benefits, avoiding pollution, and reducing production costs
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Embodiment 1
[0021] A method for extracting transparent low-acyl gellan gum, comprising the following preparation steps:
[0022] In the first step, the weight ratio of the mixed bacterial solution (Sphingomonas pauciformis solution (ATCC31461) and Bacillus pumilus solution (ATCC27142) is 8:1, and the concentration of bacteria in the two bacterial solutions is 1×10 8 Individual / mL) according to the inoculum amount of 8% (volume ratio), insert in the seed tank and cultivate, under the condition of 30 ℃ of temperature, pH value 7.2, cultivate for 24 hours to obtain liquid A, then press liquid A: fermenter culture The base was transferred to a fermenter with a volume ratio of 1:10 for cultivation at a temperature of 30°C, a pH value of 7.2, and a cultivation time of 72 hours to obtain a fermentation broth; wherein, the components of the seed tank culture medium were: 25g of sucrose, 5g of peptone, sulfuric acid Ammonium 4.0g, yeast extract 3.0g, citric acid 3.5g, calcium carbonate 2.0g, disti...
Embodiment 2
[0029] The performance parameters of the transparent type low acyl gellan gum prepared in embodiment 1:
[0030] Control group: Gellan gum was produced by fermentation only with Sphingomonas paucimobilis, and the other steps were the same as in Example 1; the experimental group was the gellan gum prepared in Example 1. Determination of molecular weight (determination of acyl group content in gellan gum by acid-base titration method): the molecular weight of the low acyl group in the control group is 710,000 Daltons, and the average molecular weight of the gellan gum in the experimental group is 720,000 Daltons.
[0031] Determination of gel strength: use TA.TX21 physical property tester to measure the light transmittance;
[0032] Measurement of light transmittance: Weigh 0.5 sample, add 100ml of distilled water, place the beaker in a water bath at 80 degrees Celsius, add 2.7% calcium chloride solution 2ml after the sample is dissolved, replenish the evaporated water to the or...
Embodiment 3
[0035] The test of embodiment 3 pig feed of the present invention:
[0036] After testing, the pig feed prepared in Example 1 has a protein content of 35.2%, a polysaccharide content of 24.1%, an inorganic mineral content of 2.7%, and the rest are starch, cellulose and a small amount of trace elements.
[0037] Choose 120 weaned piglets one month old, be divided into two groups, every group of 60, wherein experimental group is raised with the feed prepared by the present invention, and every 50kg is 210 yuan, and matched group is with Chia Tai feed (SSB-25 model) , according to the calculation of 300 yuan per 50kg. See Table 1 for the indicators detected after 6 weeks of feeding.
[0038] Table 1
[0039] Index (per piglet)
[0040] Conclusion: The cost of the pig feed prepared by using waste materials in the present invention is obviously lower than the feed commonly used in the market, and the increase in body weight is also greater than that of the control group...
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