Extraction method of cordyceps sinensis intracellular polysaccharide

A technology of intracellular polysaccharides and Cordyceps sinensis, which is applied in the field of extraction of intracellular polysaccharides from Cordyceps sinensis, can solve the problems of poor distribution, unsatisfactory extraction rate, and low activity

Inactive Publication Date: 2013-12-11
王辉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are still many defects, such as unsatisfactory extraction rat

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  • Extraction method of cordyceps sinensis intracellular polysaccharide
  • Extraction method of cordyceps sinensis intracellular polysaccharide
  • Extraction method of cordyceps sinensis intracellular polysaccharide

Examples

Experimental program
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Example Embodiment

[0086] Example 1

[0087] 1) Water extraction and alcohol precipitation of the bacterial powder to obtain crude polysaccharides: Take 100g of Cordyceps sinensis powder and perform water extraction at an extraction temperature of 100°C, an extraction time of 90 minutes, a material-to-liquid ratio of 1:10 (g / mL), and an extraction frequency of 3 The extracted crude polysaccharide solution was concentrated and precipitated with 4 times the volume of 95% ethanol at 4℃ for 24h, 8000rpm / min refrigerated centrifugation for 10min, washed twice with absolute ethanol, acetone, ether, and dried at 50℃ dry;

[0088] 2) Degrease and deproteinize crude polysaccharides: add the crude polysaccharides obtained by drying into anhydrous ethanol at a material-to-liquid ratio of 1:8 (g / mL). After degreasing at room temperature for 8 hours, distill under reduced pressure until there is no alcohol, add water Prepare a crude polysaccharide solution with a mass concentration of 1%, adjust the pH to 8-9, h...

Example Embodiment

[0093] Example 2

[0094] 1) Water extraction and alcohol precipitation of the bacterial powder to obtain crude polysaccharides: take the Cordyceps sinensis powder and perform water extraction at an extraction temperature of 100°C, an extraction time of 90 minutes, a material-to-liquid ratio of 1:10 (g / mL), and an extraction frequency of 3 times ; The extracted crude polysaccharide solution was concentrated and precipitated with 6 times the volume of 95% ethanol at 4℃ for 20h, 10000rpm / min refrigerated centrifugation for 5min, washed twice with absolute ethanol, acetone, ether, and dried at 50℃ ;

[0095] 2) Degreasing and deproteinizing crude polysaccharides: add the crude polysaccharides obtained by drying into anhydrous ethanol at a material-to-liquid ratio of 1:12 (g / mL). After degreasing at room temperature for 12 hours, distill under reduced pressure until there is no alcohol, and add water Prepare a crude polysaccharide solution with a mass concentration of 1%, adjust the p...

Example Embodiment

[0100] Example 3

[0101] Antioxidant function animal experiment:

[0102] 1. Experimental drugs

[0103] The medicine of the present invention: Cordyceps polysaccharide prepared according to Example 1.

[0104] Control drugs: decolorized polysaccharides, MPS1 and MPS2 prepared according to Example 1 of CN2008101162192.

[0105] The above-mentioned drugs were prepared into solutions of corresponding concentrations with distilled water, and the animals were gavaged at 2% (that is, 2ml per 100g body weight).

[0106] 2. Experimental animals

[0107] There are 80 female mice of Kunming species, including 70 mice over 12 months old, weighing 41-54g, and 10 mice aged 8 weeks, with an average body weight of 21.5±1.2g.

[0108] 3. Main instruments and reagents

[0109] 751 UV spectrophotometer, constant temperature water bath, micro-adjustable sampler, high-speed centrifuge and low-speed centrifuge, vortex mixer. MAD and SOD determination kits were purchased from Nanjing Jiancheng Institute of Bi...

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Abstract

The invention provides an extraction method of cordyceps sinensis intracellular polysaccharide. The extraction method comprises the steps of: performing water extraction and alcohol precipitation of fungal powder to obtain crude polysaccharide; degreasing, deproteinizing and dialyzing the crude polysaccharide; and performing column chromatography purification to obtain fine polysaccharide. The fine polysaccharide is high in extraction rate, good in purity and excellent in antioxidant effect, and the antioxidant effect of the fine polysaccharide is far better than that of the cordyceps sinensis polysaccharide extracted by the prior art.

Description

technical field [0001] The invention relates to the field of extracting active components of traditional Chinese medicines, in particular to a method for extracting intracellular polysaccharides from Cordyceps sinensis. Background technique [0002] Cordyceps sinensis is a complex of insects and bacteria formed after the larvae of Hepialus armoricamus Oberthur of the Lepidoptera genus Hepialus armoricamus are infected with the fungus Mortierella chinensis, and the complex is formed after a large number of reproductions in the body, that is, the larvae of the genus Hepialus armoricamus Oberthur. It reproduces continuously in its body until it dies and becomes a zombie, and finally grows a sub-seat to form a natural Cordyceps sinensis. From the perspective of the formation process of natural Cordyceps sinensis, Trichosporum sinensis continuously absorbs nutrients through the larvae during the reproduction process in the bat moth larvae, that is, the nutrient supply is in a dyn...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P39/06
Inventor 王玉花王辉樊国军谢建军杨成香
Owner 王辉
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