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Extraction method of cordyceps sinensis intracellular polysaccharide

A technology of intracellular polysaccharides and Cordyceps sinensis, which is applied in the field of extraction of intracellular polysaccharides from Cordyceps sinensis, can solve the problems of poor distribution, unsatisfactory extraction rate, and low activity

Inactive Publication Date: 2013-12-11
王辉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still many defects, such as unsatisfactory extraction rate, insufficient polysaccharide purity, poor distribution, low activity, etc.

Method used

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  • Extraction method of cordyceps sinensis intracellular polysaccharide
  • Extraction method of cordyceps sinensis intracellular polysaccharide
  • Extraction method of cordyceps sinensis intracellular polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] 1) Extraction and alcohol precipitation of bacterial powder to obtain crude polysaccharide: Take 100g of Cordyceps sinensis bacterial powder and carry out water extraction, the extraction temperature is 100°C, the extraction time is 90min, the ratio of solid to liquid is 1:10 (g / mL), and the number of extractions is 3 times; the extracted crude polysaccharide solution was concentrated and then precipitated with 4 times the volume of 95% ethanol at 4°C for 24 hours, refrigerated and centrifuged at 8000 rpm / min for 10 minutes, washed twice with absolute ethanol, acetone, and ether, and dried at 50°C Dry;

[0088] 2) Degrease and deproteinize the crude polysaccharide: add the crude polysaccharide obtained by drying into absolute ethanol with a solid-liquid ratio of 1:8 (g / mL), degrease at room temperature for 8 hours, distill under reduced pressure until there is no alcohol smell, add water Prepare a crude polysaccharide solution with a mass concentration of 1%, adjust the...

Embodiment 2

[0094] 1) Water extraction and alcohol precipitation of bacterial powder to obtain crude polysaccharide: take Cordyceps sinensis bacterial powder and carry out water extraction, the extraction temperature is 100°C, the extraction time is 90 minutes, the ratio of solid to liquid is 1:10 (g / mL), and the number of extractions is 3 times ; The extracted crude polysaccharide solution was concentrated and then precipitated with 6 times the volume of 95% ethanol at 4°C for 20 hours, refrigerated and centrifuged at 10,000 rpm / min for 5 minutes, washed twice with absolute ethanol, acetone, and ether, and dried at 50°C ;

[0095] 2) Degrease and deproteinize the crude polysaccharide: Add the crude polysaccharide obtained by drying into absolute ethanol with a solid-liquid ratio of 1:12 (g / mL), degrease at room temperature for 12 hours, distill under reduced pressure until there is no alcohol smell, add water Prepare a crude polysaccharide solution with a mass concentration of 1%, adjust...

Embodiment 3

[0101] Antioxidant function animal experiments:

[0102] 1. Experimental drugs

[0103] Medicine of the present invention: be the cordyceps polysaccharide that is made according to embodiment 1.

[0104] Drugs for the control group: decolorized polysaccharides, MPS1 and MPS2 prepared according to Example 1 of CN2008101162192.

[0105] Each of the above medicines was prepared into a solution of corresponding concentration with distilled water, and was given to animals by gavage at 2% (ie, 2 ml per 100 g body weight).

[0106] 2. Experimental animals

[0107] There were 80 Kunming female mice, including 70 mice over 12 months old, weighing 41-54g, and 10 mice aged 8 weeks, with an average weight of 21.5±1.2g.

[0108] 3. Main instruments and reagents

[0109] 751 ultraviolet spectrophotometer, constant temperature water bath, micro-adjustable sampler, high-speed centrifuge and low-speed centrifuge, vortex mixer. MAD and SOD assay kits were purchased from Nanjing Jiancheng I...

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Abstract

The invention provides an extraction method of cordyceps sinensis intracellular polysaccharide. The extraction method comprises the steps of: performing water extraction and alcohol precipitation of fungal powder to obtain crude polysaccharide; degreasing, deproteinizing and dialyzing the crude polysaccharide; and performing column chromatography purification to obtain fine polysaccharide. The fine polysaccharide is high in extraction rate, good in purity and excellent in antioxidant effect, and the antioxidant effect of the fine polysaccharide is far better than that of the cordyceps sinensis polysaccharide extracted by the prior art.

Description

technical field [0001] The invention relates to the field of extracting active components of traditional Chinese medicines, in particular to a method for extracting intracellular polysaccharides from Cordyceps sinensis. Background technique [0002] Cordyceps sinensis is a complex of insects and bacteria formed after the larvae of Hepialus armoricamus Oberthur of the Lepidoptera genus Hepialus armoricamus are infected with the fungus Mortierella chinensis, and the complex is formed after a large number of reproductions in the body, that is, the larvae of the genus Hepialus armoricamus Oberthur. It reproduces continuously in its body until it dies and becomes a zombie, and finally grows a sub-seat to form a natural Cordyceps sinensis. From the perspective of the formation process of natural Cordyceps sinensis, Trichosporum sinensis continuously absorbs nutrients through the larvae during the reproduction process in the bat moth larvae, that is, the nutrient supply is in a dyn...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P39/06
Inventor 王玉花王辉樊国军谢建军杨成香
Owner 王辉
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