Method for preparing ferroferric oxide/gold nano-composite particles of star-like structure in micro-emulsion
A technology of triiron tetroxide and composite particles, which is applied in the field of nanomaterials, can solve the problems of long use time and difficult control, and achieves the effects of convenient morphology, simple steps and mild reaction conditions.
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Embodiment 1
[0025] Experimental Materials
[0026] Cyclohexane, polyethylene glycol octylphenyl ether, n-hexanol, Fe 3 o 4 Nanoparticles, HAuCl 4 , Hydroxylamine.
[0027] Experimental procedure
[0028] The first step: prepare two water-in-oil microemulsions: each part is prepared from 33.33 ml cyclohexane, 10 ml polyethylene glycol octylphenyl ether and 6.67 ml n-hexanol (wherein n-hexanol and polyethylene glycol The volume ratio of alcohol octyl phenyl ether is 1:1.5, the volume ratio of the mixture of n-hexanol and polyethylene glycol octyl phenyl ether to cyclohexane is 1:2), mix and stir for 5 min.
[0029] Step 2: Slowly add 2ml (4% of the total volume of microemulsion) HAuCl to a portion of water-in-oil microemulsion 4 Aqueous solution, HAuCl 4 The aqueous solution has 7 different concentrations, namely 20 mM, 30 mM, 40 mM, 60 mM, 80 mM, 100 mM and 120 mM. Stir for 10 min to mix well to make HAuCl 4 For microemulsion, slowly add 2ml (4% of the total volume of microe...
Embodiment 2
[0043] in Fe 3 o 4 A coating layer is prepared on the surface of the nanoparticles, and the coating layer is SiO 2
[0044] Experimental Materials
[0045] Fe 3 o 4 Nanoparticles, Tetraethyl Orthosilicate, Absolute Ethanol, NaOH
[0046] Experimental procedure
[0047] Use NaOH to adjust the pH value of deionized water to 8-9, and the water-soluble Fe 3 o 4 Nanoparticles are dispersed therein to form a suspension at a concentration of 1 mg / ml. Tetraethyl orthosilicate was added to make Fe 3 o 4 The volume ratio of the suspension to tetraethyl orthosilicate is 10:1, shake and mix for 2-3 hours. After washing with ethanol and deionized water, the coated SiO 2 Fe 3 o 4 Nanoparticles.
[0048] Coated SiO 2 Fe 3 o 4 Synthesis of Fe by Nanoparticles in Microemulsion 3 o 4 / Au nanocomposite particles, the synthesis method is the same as in Example 1.
Embodiment 3
[0050] in Fe 3 o 4 A coating layer is prepared on the surface of the nanoparticles, and the coating layer is bovine serum albumin
[0051] Experimental Materials
[0052] Fe 3 o 4 Nanoparticles, bovine serum albumin (BSA), ethanol, glutaraldehyde
[0053] Experimental procedure
[0054] 200ul 0.1mg / ml water-soluble Fe 3 o 4 The nanoparticle suspension was dispersed in 2 mg / ml bovine serum albumin (BSA) aqueous solution such that the total volume was 5 ml. Under the action of ultrasound, slowly add 5 ml ethanol dropwise to Fe 3 o 4 20 ul of 5% glutaraldehyde was finally added to the BSA suspension. Ethanol was removed by rotary evaporation and centrifuged to obtain Fe coated with BSA 3 o 4 Nanoparticles.
[0055] Fe coated with BSA 3 o 4 Synthesis of Fe by Nanoparticles in Microemulsion 3 o 4 / Au nanocomposite particles.
[0056] Experimental Materials
[0057] Cyclohexane, polyethylene glycol octylphenyl ether, n-hexanol, Fe coated with BSA 3 o ...
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