Method for synchronously extracting polysaccharides and proteins from high-temperature peanut meal
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A technology of hot-pressed peanut meal and simultaneous extraction, which is applied in peptide preparation methods, chemical instruments and methods, organic chemistry, etc., to achieve the effects of high digestibility, quality improvement, and high nitrogen solubility index
Active Publication Date: 2013-12-25
INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
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Problems solved by technology
The preparation of proteins and polysaccharides mainly uses chemical methods. Although the extracted samples are of high purity, during the extraction process, due to the use of high concentrations of chemical reagents, they have a damaging effect on amino acids, resulting in denaturation of proteins and polysaccharides to a certain extent. Toxic compounds will be produced, and the protein and polysaccharides mentioned are not suitable for consumption
At the same time, due to the consumption of a large amount of chemical reagents during extraction, the burden of wastewater treatment is large, so it is difficult to be used in industrial production
Method used
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Examples
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Effect test
Embodiment 1
[0073] (1) Raw material pretreatment
[0074] The hot-pressed peanut meal is ultra-finely crushed to 800-1000 mesh, and 100g of hot-pressed peanut meal is taken as the raw material, and the protein content is 46.13 g and the polysaccharide content is 32.33 g;
[0075] (2) Enzymatic hydrolysis of fat
[0076] The crushed peanut meal is mixed with water. The weight ratio of peanut meal to water is 1:8, and Rhizopus deliema lipase is added. The addition amount of Rhizopus deliema lipase is 0.3%, and the enzymatic hydrolysis temperature is 40. ℃, adjust pH 5, after enzymolysis for 1.8h, inactivate enzyme at 100℃ for 6min;
[0077] (3) Enzymatic hydrolysis of fiber
[0078] Cellobiohydrolase enzyme addition amount 0.7%, enzymolysis temperature 40℃, pH5, after enzymolysis 1.6h, inactivate enzyme at 100℃ for 8min;
[0079] (4) Conversion of non-water-soluble polysaccharides
[0080] The addition amount of Bacillus subtilis saccharification meso-temperature α-amylase is 0.9%, enzymatic hydrolysi...
Embodiment 2
[0108] The difference from Example 1 is that in this example, the amylase used is Rhizopus alpha-amylase, and its addition amount is the same as that of Example 1, and the other conditions are exactly the same.
[0109] The protein extraction rate is 95.13%;
[0110] The extraction rate of polysaccharides is 91.83%.
Embodiment 3
[0112] The difference from Example 1 is that in this example, the cellulase used is β-1,4-glucanase, and the amount added is the same as that of the cellulase in Example 1. The other conditions are exactly the same;
[0113] The protein extraction rate was 95.37%;
[0114] The extraction rate of polysaccharides is 91.26%.
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Abstract
The invention belongs to the technical field of food processing, and particularly relates to a method for synchronously extracting polysaccharides and proteins from high-temperature peanut meal. The method for synchronously extracting polysaccharides and proteins from the high-temperature peanut meal comprises the following steps: (1) pretreatment of raw materials; (2) fat enzymolysis; (3) fiber enzymolysis; (4) water-insoluble polysaccharide conversion; (5) centrifugal separation; (6) soluble polysaccharide extraction; (7) protein extraction. The action conditions of adopted enzymes are mild, fats and celluloses in the high-temperature peanut meal are subjected to enzymolysis by using various different enzymes, and polysaccharides and proteins in the high-temperature peanut meal are extracted, thereby improving the utilization rate of the peanut meal; polysaccharides and proteins obtained by using the method disclosed by the invention are not only high in yield, but also high in purity.
Description
technical field [0001] The invention belongs to the technical field of food processing, and in particular relates to a method for synchronously extracting polysaccharide and protein from hot-pressed peanut meal. Background technique [0002] Hot-pressed peanut meal is the main by-product produced in the processing of peanut oil and other peanut products. It has abundant sources. The annual output of peanut meal in my country reaches about 9 million tons. The protein content in hot-pressed peanut meal is rich, reaching 50%. Peanut protein It almost includes eight kinds of amino acids necessary for the human body, glutamic acid and aspartic acid are relatively high, its nutritional value is similar to that of animal protein, its protein content is higher than that of crucian carp, lean pork, and eggs, and it does not contain cholesterol and has high digestibility , so it has rich nutritional value. Compared with the widely used soybean protein, peanut protein has the advantage...
Claims
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Application Information
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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C07K1/14
Inventor 杜方岭陶海腾徐同成刘丽娜邱斌刘玮韩伟高新昊
Owner INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
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