Method for synchronously separating lotus leaf flavone and chlorophyll from fresh lotus leaves

A technology for synchronous separation of lotus leaf flavonoids, applied in chemical instruments and methods, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the waste of lotus leaf chlorophyll, low content of lotus leaf flavonoids, high content of impurities in lotus leaf flavonoids, etc. problems, to achieve the effect of improving purity and yield, increasing added value, and avoiding pollution

Active Publication Date: 2014-01-01
江苏科乐欣生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, most of the lotus leaf flavonoids are extracted from dried lotus leaves, and fresh lotus leaves need to be dried and crushed, which not only increases the operation steps of the extraction process, but also wastes the chlorophyll rich in lotus leaves
In addition, in the existing extraction methods of lotus leaf flavonoids, water extraction or organic solvent extraction is mainly used to extract lotus leaf flavonoids f

Method used

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  • Method for synchronously separating lotus leaf flavone and chlorophyll from fresh lotus leaves
  • Method for synchronously separating lotus leaf flavone and chlorophyll from fresh lotus leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Crush the fresh lotus leaves, add 1 part by weight of fresh lotus leaves and 15 parts by weight of ethanol with a mass concentration of 90% in the extraction tank, extract 3 times at 50°C for 1.5 hours each time, combine the extracts, and add to the extracts Petroleum ether is added to the mixture, and the volume ratio of the extract to the added petroleum ether is 3:1, stirred, and left to stand to obtain an extract layer containing petroleum ether and chlorophyll and a layer containing ethanol, lotus leaf flavonoids, lotus leaf alkaloids and polysaccharides. Raffinate layer.

[0026] The extract containing petroleum ether and chlorophyll is concentrated under reduced pressure at a vacuum degree of 700mmHg to obtain a green oily liquid, and the green oily liquid is subjected to microwave vacuum drying at a vacuum degree of 740mmhg and a temperature of 60°C to obtain a chlorophyll powder. , the color value of the obtained chlorophyll reaches 240, and the yield can reach...

Embodiment 2

[0030] Crush the fresh lotus leaves, add 1 part by weight of fresh lotus leaves and 8 parts by weight of ethanol with a mass concentration of 95% in the extraction tank, extract 4 times at 80°C for 2 hours each time, combine the extracts, and add to the extracts Add sherwood oil, the volume ratio of the extract and the added sherwood oil is 2:1, stir, leave standstill, obtain the extract liquid layer that contains petroleum ether and chlorophyll and contain ethanol, lotus leaf flavonoids, lotus leaf alkaloids and polysaccharide Raffinate layer.

[0031] The extract containing petroleum ether and chlorophyll is concentrated under reduced pressure at a vacuum degree of 750mmHg to obtain a green oily liquid, and the green oily liquid is subjected to microwave vacuum drying at a vacuum degree of 760mmhg and a temperature of 55°C to obtain a chlorophyll powder. , the color value of the obtained chlorophyll reaches 260, and the yield can reach 92% (yield=quality of chlorophyll obtai...

Embodiment 3

[0036] Crush the fresh lotus leaves, add 1 part by weight of fresh lotus leaves and 25 parts by weight of ethanol with a mass concentration of 90% in the extraction tank, extract twice at 80°C for 1 hour each time, combine the extracts, and add to the extraction tank Add petroleum ether to the liquid, the volume ratio of the extract to the added petroleum ether is 3:1, stir, and let it stand to obtain an extract layer containing petroleum ether and chlorophyll and containing ethanol, lotus leaf flavonoids, lotus leaf alkaloids and polysaccharides raffinate layer.

[0037] Concentrate the extract containing petroleum ether and chlorophyll under reduced pressure at a vacuum degree of 800mmHg to obtain a green oily liquid, and dry the green oily liquid in a microwave vacuum at a vacuum degree of 800mmhg and a temperature of 50°C to obtain a chlorophyll powder. , the color value of the obtained chlorophyll reaches 210, and the yield can reach 90.7% (yield=quality of chlorophyll ob...

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Abstract

The invention discloses a method for synchronously separating lotus leaf flavone and chlorophyll from fresh lotus leaves. The method comprises the following steps: a, adding the fresh lotus leaves and ethanol with the concentration of 90-95% into an extraction tank, extracting for 2-4 times at the temperature of 30-80 DEG C, wherein each extraction time is 1-2 hours, and mixing the extracting solutions; b, adding ligarine into the extracting solutions, stirring and performing still standing to obtain an extraction liquid layer and a raffinate layer; c, performing concentration on an extraction liquid obtained in the step b in a pressure reduction condition to obtain a green oily liquid; d, allowing a raffinate obtained in the step b to pass an ultrafiltration membrane for ultrafiltration so as to obtain a concentrated solution processed by the ultrafiltration membrane; e, adding the concentrated solution obtained in the step d into purified water for dilution, allowing the diluted solution to pass a macroporous adsorption resin column to enable the lotus leaf flavone to be adsorbed on the macroporous adsorption resin column, and obtaining the lotus leaf flavone through leaching, concentrating and drying. The method can synchronously separate the lotus leaf flavone and the chlorophyll from the fresh lotus leaves, is simple and feasible and low in cost, and adapts to industrial application.

Description

technical field [0001] The invention relates to a method for extracting lotus leaf extract, in particular to a method for synchronously separating lotus leaf flavonoids and chlorophyll from lotus leaves. Background technique [0002] The lotus leaf is the leaf of the water lily family plant lotus, and is a plant leaf with both medicine and food. The lotus leaf contains active substances such as flavonoids and alkaloids. Modern medical research shows that lotus leaf flavonoids have the functions of anti-oxidation, anti-aging, clearing away heat, lowering blood fat, lowering cholesterol, and treating cardiovascular and cerebrovascular diseases. Lotus leaf alkaloids have the functions of lowering fat and losing weight, antibacterial and antiviral. The lotus leaf also contains polysaccharides, which have special biological activity and are an immunomodulator without toxic side effects, and can be used as clinical medicine or vaccine. As a natural pigment, chlorophyll in lotus...

Claims

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Application Information

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IPC IPC(8): A61K36/62A23L1/29C07D487/22C07G5/00C08B37/00A61K127/00A23L33/00
Inventor 徐君周爱琴
Owner 江苏科乐欣生物有限公司
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