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Preparation method and application of pig vaccine-specific bovine spleen transfer factor

A transfer factor and specific technology, which is applied in the field of preparation of swine vaccine-specific bovine spleen transfer factor, can solve the problems of difficulty in large-scale production, TF is not suitable for swine vaccine diluent and immune adjuvant, and the production cycle is long. The effect of reducing the number of freeze-drying operations, reducing the volume of freeze-drying, and reducing the volume used

Active Publication Date: 2015-12-09
派生特(福州)生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Infection of suckling piglets within 7 days of age is the most common. In severe cases, it can lead to the disease of the whole group, resulting in the death of a large number of suckling piglets. The weight gain of nursery pigs and fattening pigs slows down, causing great losses to the pig industry.
The Lawrence method is routinely used, that is, the transfer factor is recovered by two dialysis methods. This method is simple to operate, but the production cycle is long, time-consuming, wasteful, ineffective and labor-intensive.
In addition, even if the tangential flow filtration method is adopted, hollow fiber membranes are mostly used for tangential flow filtration. The hollow fiber membranes are difficult to clean, and the entire component needs to be replaced after the membrane is damaged. The residual dead volume of the system is large, and the flow resistance in the fiber tube is very large. , Large pressure loss, not suitable for handling viscous liquids, not easy to linearly scale up production
These methods are more difficult to scale up production to meet the needs of the market
[0021] In the original method of preparing TF, due to the selection of formaldehyde inactivation and the lack of osmotic pressure adjustment process in the inactivation process, the prepared TF is not suitable for use as a diluent and immune adjuvant for porcine vaccines, that is, it cannot directly dilute porcine attenuated vaccines, and cannot be used with Porcine attenuated vaccine virus solution mixed and freeze-dried

Method used

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  • Preparation method and application of pig vaccine-specific bovine spleen transfer factor
  • Preparation method and application of pig vaccine-specific bovine spleen transfer factor
  • Preparation method and application of pig vaccine-specific bovine spleen transfer factor

Examples

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Effect test

preparation example Construction

[0075] The preparation method of porcine vaccine-specific bovine spleen transfer factor, it comprises the following steps:

[0076] 1) Feed healthy cows with white lentils at a dosage of 50-100g per cow per day for 15-30 days, and then immunize healthy cows fed with white lentils with more than one pig vaccine, each pig vaccine immunized twice Above, the time interval between two adjacent pig vaccine immunizations is more than 7 days, and 14-28 days after the last pig vaccine immunization, the pig vaccine antibody is detected, the pig vaccine antibody-positive cattle are selected for slaughter, and the spleen of the pig is harvested;

[0077] 2) Low-temperature preservation: the harvested bovine spleen should be stored immediately below -40°C, and the storage time should not exceed 20 days;

[0078] 3) Thawing bovine spleen: thaw the cryopreserved bovine spleen through running water, the thawing temperature is lower than 37°C, wash with purified water 3-5 times after thawing, ...

Embodiment 1

[0149] The preparation method of porcine vaccine-specific bovine spleen transfer factor, it comprises the following steps:

[0150] 1) Feed healthy cows with white lentils at a dosage of 60g per cow per day for 18 days, then immunize the healthy cows fed white lentils with porcine reproductive and respiratory syndrome vaccine more than 3 times, and immunize pigs twice adjacent The time interval of PRRS vaccine is 7 days. 28 days after the last PRRS vaccine immunization, the PRRS antibody is detected, and the cattle positive for PRRS antibody are selected for slaughter and harvested. bovine spleen;

[0151] 2) Low-temperature preservation: the harvested bovine spleen is immediately stored at -40°C, and the storage time does not exceed 20 days;

[0152] 3) Thawing bovine spleen: thaw the cryopreserved bovine spleen through running water, the thawing temperature is lower than 37°C, wash with purified water 3 times after thawing, and discard the hardened and necrotic bovine splee...

Embodiment 2

[0177] The preparation method of porcine vaccine-specific bovine spleen transfer factor, it comprises the following steps:

[0178] 1) Feed healthy cattle with white lentils, the dosage is 80g per cow per day, and feed for 21 days, and then immunize the healthy cattle fed with white lentils with swine fever vaccine and porcine reproductive and respiratory syndrome vaccine in turn, each pig vaccine immunization More than 3 times, the time interval between two adjacent immunizations is 10 days, 20 days after the last immunization with pig vaccine, detect pig vaccine antibody, select cattle positive for swine fever or porcine reproductive and respiratory syndrome antibody, slaughter, and harvest cattle spleen;

[0179] 2) Low-temperature preservation: the harvested bovine spleen is immediately stored at -80°C, and the storage time does not exceed 20 days;

[0180] 3) Thawing bovine spleen: thaw the cryopreserved bovine spleen through running water, the thawing temperature is lowe...

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Abstract

The invention discloses a preparation method of a swine vaccine specific cattle spleen transfer factor (TF). The preparation method comprises the following steps: slaughtering a cattle with a positive swine vaccine antibody to harvest the cattle spleen; preserving the cattle spleen at low temperature; unfreezing the cattle spleen; removing fasciae; mincing the cattle spleen; homogenizing the pulp; filling the homogenized pulp into a bottle and storing the pulp; repeatedly freezing and thawing the pulp; centrifuging the pulp; carrying out microfiltration; carrying out ultrafiltration; carrying out inactivation; regulating the pH value; regulating the osmotic pressure; removing bacteria; and detecting the quality. The preparation method has the advantages that the pH value of water for injection is regulated to 4-6 in the pulp homogenizing step, thus being beneficial to increasing of the yields of ribose and polypeptide; a box type membrane coating is adopted for tangential flow filtration, so that the dead volumes of system residues are small and linear amplification production is easy to achieve; a 1-3KD box type membrane coating is adopted to carry out tangential flow nanofiltration on a TF crude product, thus preparing the high-concentration TF and improving the using effects; phenol red is used as an acid-base indicator, thus being convenient for clients to observe the pH value of the TF; the TF uses beta-propiolactone for inactivation instead of traditional formaldehyde and an osmotic pressure regulation process is added, thus being beneficial to combined immunization of the TF and vaccines.

Description

technical field [0001] The invention belongs to transfer factor, and in particular relates to a preparation method and application of porcine vaccine-specific bovine spleen transfer factor. Background technique [0002] Transfer factor (TransferFactor, TF) is a substance released by T lymphocytes that can transfer sensitization information. It can specifically transfer the donor's cellular immune information to the recipient, thereby enhancing the immune function of the recipient. Since the discovery of TF in the 1950s, scholars at home and abroad have conducted a lot of research on it, and introduced it into veterinary immunity and clinical treatment in 1979. At present, TF has gradually become a widely used immune enhancer. [0003] TF has no species specificity, so TF has a wide range of sources. Human TF, pig TF, cattle TF, sheep TF, goose TF, monkey TF, etc. are common in human clinical practice, while bovine TF and pig TF are common in livestock and poultry breeding. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/39A61P37/04A61K35/26
Inventor 徐磊游晓军刘友霖
Owner 派生特(福州)生物科技有限公司