Thermo-sensitive gel composition and application thereof
A temperature-sensitive gel and composition technology, applied in medical science, surgery, internal accessories, etc., can solve the problems of inevitable liver ischemia-reperfusion injury, inaccurate partial excavation, and complicated operation, so as to reduce recurrence and improve safety sex, reduce bleeding
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Embodiment 1
[0051] Embodiment 1: rat portal vein injection experiment
[0052] 1. Reagents and Instruments
[0053] Reagents: Poloxamer 407 (P407, BASF, Germany); Indocyanine Green (ICG, Dandong Yichuang Pharmaceutical Co., Ltd.).
[0054] Instruments: collector type constant temperature heating magnetic stirrer (Beijing Medical Equipment Factory); 501 super constant temperature water bath (Beijing Medical Equipment Factory); laser perfusion imager (Moor Equipment Co., Ltd., UK); animal surgical instruments (Shanghai Medical Equipment Co., Ltd.) factory).
[0055] 2. Experimental animals
[0056] Ten healthy male SD rats, weighing 240-260 g, were provided by the Experimental Animal Center of the Academy of Military Medical Sciences. Free access to food and water, 12 hours of lighting, and the experiment began after a week of adaptation.
[0057] 3. Preparation of novel thermosensitive gel and investigation of gelation temperature
[0058] (1) The P407 solution is prepared by cold met...
Embodiment 2
[0069] Embodiment 2: Liver segment resection experiment of Landrace pig
[0070] 1. Reagents and Instruments
[0071] Conventional abdominal surgical instruments (Shanghai, China); SERIE7200 ventilator (USA); monitor (USA); SIMENZ ultrasound machine (USA); ultrasound probe L5-12 (USA); puncture needle (USA); optical microscope (Japan); Blood Biochemical Automatic Analyzer (Germany); TS-12F Biological Tissue Automatic Dehydrator (Hubei, China); CS-Ⅳ Spreading and Slicing Machine (Hubei, China) LEICA RM2016 Paraffin Sectioning Machine (Shanghai, China) LEICA EG1120 Tissue Embedding Machine (Shanghai, China); KD-BL Embedding Machine Freezing Station (Zhejiang, China)
[0072] 2. Experimental animals
[0073] Eighteen healthy male Landrace pigs, weighing 35-40Kg, were provided by the Experimental Animal Center of the General Hospital of the People's Liberation Army and raised in single cages under the conditions of circadian rhythm and automatic room temperature control. Pigs wer...
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