Method of preparing albumin from a solution comprising albumin and a method for inactivating viruses using caprylate in solutions containing albumin

A technology of albumin and octanoate, which is applied in the field of preparing albumin from albumin-containing solution and using octanoate in albumin-containing solution to inactivate viruses, which can solve the problems of cost, explosion and fire

Inactive Publication Date: 2014-02-12
GRIFOLS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The acetone method is expensive and uses large amounts of acetone, which poses a fire or explosion hazard

Method used

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  • Method of preparing albumin from a solution comprising albumin and a method for inactivating viruses using caprylate in solutions containing albumin
  • Method of preparing albumin from a solution comprising albumin and a method for inactivating viruses using caprylate in solutions containing albumin
  • Method of preparing albumin from a solution comprising albumin and a method for inactivating viruses using caprylate in solutions containing albumin

Examples

Experimental program
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Effect test

Embodiment 1

[0050] human albumin

[0051] Suspend Cohn Fraction V in cold water for injection. Alternatively, effluent IV-1 can be used instead of fraction V. Add cold denatured ethanol (SDA-3A) to obtain an approximately 10% alcohol solution while cooling the solution to a temperature of approximately 0 °C. The solution was mixed for about 1 hour at about 0°C, then clarified by depth filtration.

[0052] The albumin fraction was then precipitated from the flow-through IV-1 or fraction V by adjusting the pH, adding cold denatured ethanol (SDA-3A), and incubating the solution at low temperature. After incubation at low temperature, the albumin fraction was isolated by centrifugation.

[0053] Acetone inactivates virus

[0054] The albumin fraction was then suspended in cold acetone and kept at about 0°C for several minutes. The protein was separated from the slurry and rinsed with cold acetone. Dry the wet albumin powder by passing dry nitrogen and / or air through the powder.

[0055...

Embodiment 2

[0061] Virus inactivation experiment

[0062] Acetone treatment of albumin slurries is a very effective enveloped virus inactivation step, but this step is a bottleneck in the process and is also associated with numerous cleaning and safety issues (fires). Therefore, caprylate treatment may replace acetone suspension and drying. Experiments were performed to evaluate the inactivation of enveloped viruses by treatment of albumin serum suspensions with octanoate. For this study, albumin slurry suspensions with a protein concentration of 25 AU were spiked with bovine viral diarrhea virus (BVDV) or vesicular stomatitis virus (VSV) and incubated at 27°C, pH 5.1, with 0 mM, 10 mM, 15 mM, or 20 mM octanoate was incubated for 1 hour. The data showed that the virus was inactivated to the limit of detection following treatment with 15 mM octanoate. See Table 1 and figure 1 .

[0063]

[0064]

Embodiment 3

[0066] Experiments were performed at low temperature to evaluate the virucidal ability of caprylate. Albumin and Fraction V serum suspensions were diluted to absorbance units (AU) of 25 and 65, and sodium caprylate was added to a final concentration of 20 mM. The solution was adjusted to pH 4.5 or 5.1 and the pH adjusted BVDV was spiked prior to incubation at 5°C, 12°C, or 20°C. Pipette for titration, pH, and / or protein concentration (i.e., A 280 )sample.

[0067] Virus inactivation by caprylate was greater at higher temperatures and lower protein concentrations for both albumin and Fraction V serum suspensions. see figure 2 . Virus inactivation was higher at pH 4.5 than at pH 5.1 for Fraction V suspensions at 25 AU and 65 AU. There was also a higher virus inactivation at pH 4.5 for the albumin suspension at 25 AU. However, virus inactivation was higher at pH 5.1 than at pH 4.5 for the albumin suspension at 65 AU. Since the mechanism of virus inactivation by caprylate ...

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Abstract

Described herein are a method of preparing albumin from a solution comprising albumin and a method for inactivating viruses using caprylate in solutions containing albumin. The method of preparing albumin from a solution comprising albumin comprising: adjusting the protein concentration of the solution to less than about 5%; adjusting pH of the solution to about pH 5 or less; adding caprylic acid (octanoic acid) or sodium caprylate; raising the solution temperature greater than about 20 DEG C; and incubating the solution.

Description

technical field [0001] Described herein is the use of octanoate to inactivate viruses during the purification of albumin from plasma. Background technique [0002] Human serum albumin (hereinafter referred to as albumin) is the most abundant protein in human plasma. Body fluid albumin is a 585 amino acid protein with a molecular weight of 67 kDa. This protein has a serum half-life of about 20 days and is involved in transporting many hormones, metabolites, and drugs as well as maintaining osmolarity and buffering blood pH. Albumin is given therapeutically in trauma, burn, and surgical patients to replace lost body fluid and restore blood volume. [0003] Cohn first described the purification of albumin from human plasma by differential fractionation. See Cohn et al., J.Am.Chem.Soc.68:459-475 (1946); Cohn et al., J.Am.Chem.Soc.69:1753-1761 (1947); US Patent Nos. 2,390,074 and 2,469,193. These methods were improved by Gerlough. See US Patent Nos. 2,710,293 and 2,710,294....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/765C12N7/06
CPCC12N2760/20263A61K47/14C07K14/76A61K47/12C07K14/765C12N7/06C12N2770/24363C12N7/00A01N37/02A61K38/38C07K1/14C12N7/04A61K2121/00
Inventor 莱宾·威托尔德伯恩斯·道格罗思·纳坦霍塔·约安
Owner GRIFOLS
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