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A method for detecting bifenthrin pesticides using fluorescent nano-cdse quantum dot probes

A fluorescent nanometer and bifenthrin technology, which is used in fluorescence/phosphorescence, material excitation analysis, etc., to achieve the effect of strong photobleaching resistance, high fluorescence intensity and high sensitivity

Active Publication Date: 2016-04-13
百通信息科技(泗阳)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, no reports have been reported on the detection of bifenthrin pesticides using fluorescent nano-CdSe quantum dot probes.

Method used

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  • A method for detecting bifenthrin pesticides using fluorescent nano-cdse quantum dot probes

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Preparation of fluorescent nanometer CdSe quantum dot probe:

[0019] Take by weighing 0.05g selenium powder and join in the flask that 0.28g tri-n-butylphosphine and 1.4g dioctylamine are housed, stand still 14h, prepare selenium stock solution; Take 0.018g cadmium oxide and 0.15g stearic acid in three In the flask, heat to 168°C under the protection of argon to fully dissolve the cadmium oxide, and prepare the cadmium stock solution; Stir and heat to 308°C, inject the selenium stock solution taken out into the reaction container, continue to react at 288°C for 90 seconds, and cool the product to room temperature to prepare fluorescent nano-CdSe quantum dot probes.

Embodiment 2

[0021] A method utilizing fluorescent nano CdSe quantum dot probes to detect bifenthrin pesticides:

[0022] In the process of using fluorescent nanometer CdSe quantum dots to detect bifenthrin pesticide in the standard product of bifenthrin pesticide, the fluorescent nanometer CdSe quantum dot probe solution diluted 25 times was mixed with the bifenthrin pesticide solution to make the fluorescent nanometer The molar ratio of the CdSe quantum dot probe solution to the bifenthrin pesticide solution is 1: 0.71, that is, figure 1 Medium Fluorescence Spectrum a.

Embodiment 3

[0024] A method utilizing fluorescent nano CdSe quantum dot probes to detect bifenthrin pesticides:

[0025] The rest of the conditions are the same as in step 1, only the molar ratio of the fluorescent nano-CdSe quantum dot probe solution to the bifenthrin pesticide solution is changed to 1: 2.8, then the obtained figure 1 Mid-fluorescence spectrum b.

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Abstract

The invention discloses a method for detecting bifenthrin pesticides by a fluorescent nanometer cadmium selenide (CdSe) quantum dot probe. Fluorescence of fluorescent nanometer CdSe quantum dot probe can be quenched by bifenthrin effectively, so that the bifenthrin pesticides in a solution can be rapidly and sensitively detected by a fluorescence quenching phenomenon, a detection limit of the bifenthrin pesticides can reach 5.1*10<-8>mol / L, and the correlation coefficient is 0.9999. The method disclosed by the invention is simple and convenient to operate, further high in sensitivity and high in selectivity, and is capable of realizing highly sensitive and selective detection of bifenthrin in mixed samples.

Description

technical field [0001] The invention belongs to the technical field of bifenthrin pesticide detection, and in particular relates to a method for detecting bifenthrin pesticide by using a fluorescent nanometer CdSe quantum dot probe. Background technique [0002] Bifenthrin belongs to a kind of pyrethroid insecticide and acaricide, with high insecticidal activity, mainly contact and stomach poisoning, without fumigation and systemic effect. Bifenthrin is a broad-spectrum insecticide with moderate toxicity to bees and high toxicity to silkworms. When bifenthrin acts on insects, it has the characteristics of rapid action and long-lasting effect. It has good control effects on many pests and a variety of spider mites, but it is highly toxic to fish. So far, chromatography and immunoassays are mainly used for the detection of bifenthrin pesticides, but these two methods have the disadvantages of cumbersome operation and high cost. Therefore, it has become a development trend to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 黄珊肖琦盛家荣苏炜盛凯朱发伟
Owner 百通信息科技(泗阳)有限公司
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