Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method of extracting and separating triterpenoid ellagitannin compound from castanopsis fissa leaves

A technology of triterpene ellagitan and pruriens, which is applied in the field of extraction of active ingredients in plants, can solve problems such as unsatisfactory yield, difficulty in industrialization, complicated process, etc., achieve simple operation, improve product yield and purity high effect

Inactive Publication Date: 2015-07-22
GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the above documents can be separated and refined to obtain triterpene ellagitannins, this method uses Sephadex LH-20, HP20SS CC and Chromatorex ODS multiple chromatography, the process is relatively complicated and the cost is high, and it is not easy to realize industrial and the yield is not very ideal in addition (the yield of fresh leaves is 0.27%)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method of extracting and separating triterpenoid ellagitannin compound from castanopsis fissa leaves
  • Method of extracting and separating triterpenoid ellagitannin compound from castanopsis fissa leaves
  • Method of extracting and separating triterpenoid ellagitannin compound from castanopsis fissa leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1) Take 6.0kg of fresh leaves of Mucuna pruriens, chop finely, add 80% acetone solution 5 times the weight of raw materials, extract at room temperature for 24 hours, filter, add 80% acetone solution 5 times the weight of raw materials to the filter residue, extract at room temperature for 12 hours, Filtrate and combine the filtrates to obtain the extract;

[0022] 2) When the extract is recovered from acetone under reduced pressure until complete, the chlorophyll and wax are removed by filtration to obtain the filtrate;

[0023] 3) Apply D101 macroporous resin chromatography to the filtrate obtained in step 2), wash the column with 4 times the resin volume of water, then elute with 4 times the resin volume of 50% ethanol, and then elute with 80% ethanol until there is no phenolic reaction (2wt% FeCl 3 For aqueous solution detection, the amount of 80% ethanol is about 7 times the volume of the resin), and the 80% ethanol eluate is collected;

[0024] 4) The collected ...

Embodiment 2

[0038] 1) Take 6.1 kg of fresh leaves of Mucuna pruriens, chop finely or pulverize, add 80% acetone solution 5 times the weight of the raw material, reflux extraction for 2 hours, filter, add 80% acetone solution 5 times the weight of the raw material to the filter residue, and reflux extraction for 2 hours, Filtrate and combine the filtrates to obtain the extract;

[0039] 2) When the extract is recovered from acetone under reduced pressure until complete, the chlorophyll and wax are removed by filtration to obtain the filtrate;

[0040] 3) HP-20 macroporous resin chromatography on the filtrate obtained in step 2), wash the column with water of 4 times the volume of the resin, then elute with 30% ethanol of 4 times the volume of the resin, and then elute with 90% ethanol until no phenol Sexual reaction (2wt% FeCl 3 For aqueous solution detection, the amount of 90% ethanol is about 6 times the volume of the resin), and the 90% ethanol eluate is collected;

[0041] 4) Concent...

Embodiment 3

[0043] 1) Take 6.5 kg of fresh leaves of Mucuna pruriens, chop finely, add 90% acetone solution 10 times the weight of the raw material, extract at room temperature for 24 hours, filter, add 90% acetone solution 5 times the weight of the raw material to the filter residue, extract at room temperature for 10 hours, Filtrate and combine the filtrates to obtain the extract;

[0044] 2) When the extract is recovered from acetone under reduced pressure until complete, the chlorophyll and wax are removed by filtration to obtain the filtrate;

[0045] 3) Apply D-4020 macroporous resin chromatography to the filtrate obtained in step 2), wash the column with 6 times the volume of the resin, then elute with 50% methanol of 5 times the volume of the resin, and then elute with 80% methanol until no phenol Sexual reaction (2wt% FeCl 3 For aqueous solution detection, the amount of 80% methanol used is about 7 times the volume of the resin), and the 80% methanol eluate is collected;

[004...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method of extracting and separating a triterpenoid ellagitannin compound from castanopsis fissa leaves. The method comprises following steps of: 1) extracting the castanopsis fissa leaves used as a raw material by using acetone to obtain an extract; 2) recovering the acetone in the extract and filtering to obtain a filtrate; 3) passing the filtrate through macroporous resin, washing with water, eluting by using 20-50% in volume of an organic solvent, then eluting by using 70-100% in volume of the organic solvent until the eluate has no reactions of phenols, and collecting the eluate of 70-100% in volume of the organic solvent; and 4) recovering the solvent in the eluate under reduced pressure until a large quantity of crystals precipitate, cooling, permitting the eluate to stand and separating the crystals. According to the method, the macroporous resin is used for chromatography and the special eluting technology is combined, and therefore the cost is reduced, the product yield which can reach over 0.37% is improved at the same time, and the purity is high. The method is simple in operation and prone to industrialization.

Description

technical field [0001] The invention relates to the technical field of extraction of effective components in plants, in particular to a method for extracting and separating triterpene ellagitannins from leaves of Cone pruriens. Background technique [0002] Castanopsis fissa (Champ.ex Benth.) Rehd.et Wils.) is a plant of the genus Cone in the family Fagaceae, also known as Castanopsis fissa, Champ. It is widely distributed in South China. It is an excellent fast-growing and high-yield native tree species in South my country. It has very rich plant resources. It is widely used in the treatment of cardiovascular and cerebrovascular diseases, hemostasis, diarrhea, and chronic ulcers among the people. Existing literature has carried out detailed research on the chemical composition of this plant (Phytochemistry, 2011,72 (16): 2006-2014), adopt 80% acetone aqueous solution to extract in the literature, wash through Sephadex LH-20 (10~100% methanol solution) elution), HP20SS CC (...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07J71/00
Inventor 沈晓琳黄永林李典鹏田中隆陈月圆刘金磊卢凤来
Owner GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com