Phenol-resistant heterotrophic nitrification-aerobic denitrification strain and applications thereof

A technology of aerobic denitrifying bacteria and heterotrophic nitrification, applied in the directions of bacteria, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of low ammonia nitrogen degradation efficiency, and achieve the effect of efficient degradation and good application prospects.

Inactive Publication Date: 2014-02-26
WUHAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention aims to solve the problem of low ammonia nitrogen degradation efficiency in phenol-containing industrial wastewater by common heterotrophic nitrifying bacteria, and aims to provide a phenol-resistant heterotrophic nitrifying-aerobic denitrifying bacteria, which can be used for coking wastewater, oil refinery wastewater and Biointensive Treatment of Ammonia Nitrogen in Tar Processing Wastewater

Method used

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  • Phenol-resistant heterotrophic nitrification-aerobic denitrification strain and applications thereof
  • Phenol-resistant heterotrophic nitrification-aerobic denitrification strain and applications thereof
  • Phenol-resistant heterotrophic nitrification-aerobic denitrification strain and applications thereof

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Experimental program
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Effect test

Embodiment 1

[0021] Acinetobacter ( Acinetobacter sp.) Isolation, purification, identification and nitrification performance of Y3

[0022] 1. Isolation and purification of strains

[0023] (1) Source of bacteria

[0024] The bacteria source was collected from the activated sludge of the coking wastewater biological treatment system of Wuhan Iron and Steel Co., Ltd.

[0025] (2) Isolation and purification of strains

[0026] The collected activated sludge was placed in a refrigerator at 4°C, and 2 mL of the supernatant was inoculated into 100 mL of sterilized LB medium (peptone 1 wt%, sodium chloride 1 wt%, yeast extract 0.5 wt%), and 30 ℃, 250r / min activation culture overnight. Then take 2mL of the culture solution and transfer it to the sterilized heterotrophic nitrification medium, and so on, and gradually increase the concentration of ammonium sulfate during the transfer process (200 mg / L, 400 mg / L, 600 mg / L, 800 mg / L, / L). During acclimatization, 500 mg / L phenol was added...

Embodiment 2

[0039] Acinetobacter ( Acinetobacter sp.) Denitrification performance of Y3

[0040] Inoculate the strain Y3 in a sterile heterotrophic denitrification medium (containing 200 mg / L phenol), culture it at 30°C and 250 r / min, and take samples at regular intervals to detect the bacterial density (OD 600 ) and residual nitrate concentration, the results are as follows figure 2 shown. Depend on figure 2 It can be seen that the reproduction rate of strain Y3 is consistent with its denitrification rate. After 36 hours, the nitrate nitrogen with an initial concentration of 182 mg / L was completely degraded, and there was no accumulation of nitrite nitrogen during the denitrification process, and the denitrification was complete. . It shows that the strain still has good denitrification ability in the presence of phenol.

Embodiment 3

[0042] Acinetobacter ( Acinetobacter sp.) Degradation of ammonia nitrogen and phenol in coking wastewater of Y3

[0043] The bacterial suspension of strain Y3 was added to coking wastewater at an inoculation amount of 5vol%, and three groups of experiments were set up to investigate the removal effect of ammonia nitrogen and phenol in coking wastewater of strain Y3:

[0044] (a) Add 45mL of coking wastewater, 45mL of inorganic salt nitrification medium, 5mL of bacterial suspension and 5mL of unsterilized activated sludge into a 250mL Erlenmeyer flask;

[0045] (b) Add 45mL of coking wastewater, 45mL of inorganic salt nitrification medium, 5mL of bacterial suspension and 5mL of sterilized activated sludge into a 250mL Erlenmeyer flask;

[0046] (c) Add 45mL of coking wastewater, 50mL of inorganic salt nitrification medium and 5mL of activated sludge into a 250mL Erlenmeyer flask.

[0047] Place them in a shaker at 30°C and 250 r / min, and take samples at regular intervals to...

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Abstract

The invention relates to a phenol-resistant heterotrophic nitrification-aerobic denitrification strain and applications thereof. The phenol-resistant heterotrophic nitrification-aerobic denitrification strain Y3 provided by the invention belongs to Acinetobacter (Acinetobacter sp.) with a number of Y3; the strain Y3 with a preservation number of CCTCC (China Center for Type Culture Collection) NO: M2013445 is preserved in the CCTCC on September 25, 2013. The strain Y3 is a gram-negative bacterium, and a bacterial colony is milky, neat in edge, circular, and centrally raised. The strain Y3 can convert ammonia nitrogen into nitrogen through heterotrophic nitrification and aerobic denitrification by taking phenol in wastewater as a carbon source without the accumulation of an intermediate product, namely nitrite nitrogen. The strain can resist the industrial wastewater environment with a phenol concentration of 1000 mg/L; through carrying out simultaneous nitrification and denitrification by taking phenol as a carbon source, the simultaneous efficient degradation of ammonia nitrogen and phenol in wastewater is realized. The strain shows good application prospects in biological enhancement treatment of coking wastewater, oil refining wastewater and tar processing wastewater.

Description

technical field [0001] The invention belongs to the technical field of environmental pollutant bioaugmentation, and specifically relates to a phenol-resistant heterotrophic nitrification-aerobic denitrification bacterium and application thereof. Background technique [0002] With the development of industrial and agricultural production and the improvement of people's living standards, the discharge of ammonia nitrogen is increasing year by year. The excessive discharge of ammonia nitrogen leads to the increasingly serious problem of eutrophication in water bodies, which has attracted widespread attention of environmental workers. The ammonia nitrogen discharged into the water body consumes dissolved oxygen, causing a large number of fish to die, and the oxidized nitrite and nitrate are converted into nitrosamines, which have carcinogenic and mutagenic properties and pose a threat to human health. Excess discharge of ammonia nitrogen may not only cause water eutrophication, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
Inventor 颜家保许龙龙武文丽陈佩袁东东余永登杨洋
Owner WUHAN UNIV OF SCI & TECH
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