High-flocculation capability bacterial strain and use thereof

A flocculation and capacity technology, applied to strains with high flocculation ability and their application fields, can solve problems such as unsatisfactory treatment effect and environmental pollution, and achieve the effect of improving sewage treatment effect, improving treatment efficiency and reducing turbidity

Active Publication Date: 2014-02-26
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because slaughter wastewater contains many types of organic matter, it causes great pollution to the environment, and the treatment effect of traditional methods is not ideal

Method used

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  • High-flocculation capability bacterial strain and use thereof
  • High-flocculation capability bacterial strain and use thereof
  • High-flocculation capability bacterial strain and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Acquisition and Identification of Bacillus muralis D6

[0022] (1) Screening method of D6

[0023] Bacterial strains were isolated from the bottom sludge of a meat and poultry processing factory in Shenyang and the activated sludge of the aeration tank by dilution coating and plate streaking method, and 55 strains were isolated and purified. After preliminary screening, 15 strains were fermented The product can cause flocculation of kaolin suspension, and flocs are produced, which proves that the fermentation product has flocculation characteristics. The 15 strains obtained after the primary screening were re-screened, and the flocculation ability of the microbial flocculant was characterized by the flocculation rate. The flocculation rate is the largest, which is 96.77%.

[0024] Table 1 Re-screening results

[0025]

[0026] (2) Determination of the growth curve of strain D6

[0027] Liquid medium formula: 1000mL distilled water, 3g beef extract, 10g ...

Embodiment 2

[0048] Example 2 Determination and application of optimal flocculation conditions for degradation of slaughter wastewater

[0049] (1) Preparation of microbial flocculant: Insert strain D6 into 50mL fermentation medium (1000mL distilled water, 20g soluble starch, KH 2 PO 4 2g,K 2 HPO 4 5g, (NH 4 ) 2 SO 4 0.2g, NaCl 0.1g, urea 0.5g, yeast extract 0.5g, MgSO 4 ·7H 2 O 0.2g, initial pH 7.0, sterilized at 121°C for 20min) in a 250mL Erlenmeyer flask, cultured in a constant temperature shaking incubator with an oscillator temperature of 30°C and a rotation speed of 160r / min for 72h. After cultivation, the fermented liquid was centrifuged at 4000r / min for 30min with a centrifuge, and the supernatant was taken as the microbial flocculant.

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Abstract

The invention relates to a high-flocculation capability bacterial strain and a use thereof. The high-flocculation capability bacterial strain is Bacillusmuralis D6 and the metabolite of the Bacillusmuralis D6 is used as a main active component and is used as a microbial flocculant. The microbial flocculant is used for degrading slaughter wastewater and has the characteristics of nontoxicity and innocuousness, natural degradation, good treatment effects and no secondary pollution. Under the optimal addition combination conditions, the microbial flocculant produced by Bacillusmuralis D6 fermentation has a slaughter wastewater CODSr removal rate of 92.19%, a slaughter wastewater SS removal rate of 93.06%, a slaughter wastewater turbidity removal rate of 97.23%, a slaughter wastewater TN removal rate of 35.91% and a slaughter wastewater NH4<+>-N removal rate of 38.82%, improves sewage treatment effects and provides a novel method for high-efficiency treatment on the slaughter wastewater.

Description

technical field [0001] The invention relates to a bacterial strain with high flocculation ability sieved from the sludge of a slaughterhouse sewage outlet, and the microbial flocculant (MBF) produced by the bacterial strain through fermentation has an efficient degradation effect on slaughter wastewater. [0002] A strain with high flocculation ability called in the present invention is named: Bacillus muralis (Bacillus muralis) D6, which has been preserved in the China Center for Type Culture Collection on October 7, 2013, and the number is CCTCC NO: M2013461. Background technique [0003] As a water treatment agent used earlier, chemical flocculants mainly include inorganic flocculants and organic polymer flocculants. This type of flocculant has the characteristics of low price and obvious treatment effect, so it was widely used in the early days, but the use process is easy. Causes metal ions and organic residues to cause secondary pollution. [0004] Slaughter wastewate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/07C02F103/22
Inventor 付保荣付豪李霞刘冬杨玉东左世文景逵
Owner LIAONING UNIVERSITY
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