Method for enhancing Raman spectroscopy by anion modified nanoparticles

A nanoparticle and Raman spectroscopy technology, which is applied in the field of anion-modified nanoparticle-enhanced Raman spectroscopy, can solve problems such as interference, and achieve the effects of improved sensitivity, simple and easy-to-obtain preparation methods and raw materials, and broadened application range

Active Publication Date: 2014-02-26
XIAMEN PUSHI NANO TECH CO LTD
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, the disadvantage of this method is that the thiol molecule itself will also generate a strong SERS signal that interferes with the signal of the target molecule. When applied to most common biomolecules without resonance effects, this interference will be very obvious.

Method used

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  • Method for enhancing Raman spectroscopy by anion modified nanoparticles

Examples

Experimental program
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Embodiment 1

[0038] A preparation of anion-modified nanoparticles:

[0039] figure 1 A schematic diagram of the experimental procedure for preparing anion-modified nanoparticles is given.

[0040] Taking iodide ion modified silver nanoparticles as an example, the specific preparation method is:

[0041] Take 200mL silver nitrate solution with a concentration of 1mM, heat it to boiling under stirring conditions, then add 6mL aqueous solution of sodium citrate with a mass fraction of 1%, and keep boiling slightly for 1h, the solution finally turns yellow-green. Cool naturally to room temperature to obtain a silver nanoparticle sol with a diameter of about 50 nm. Take 4.5mL silver nanoparticle sol, centrifuge at room temperature (5000r / min, 10min), remove the supernatant, and concentrate the nanoparticle sol to about 50μL. Add 50 μL of 1 mM KI, mix well, and let stand at 25°C for 20 minutes to allow iodide ions to adsorb on the surface of silver nanoparticles in a monolayer to obtain iodid...

Embodiment 2

[0045] Check whether the anion is full of monolayer modification, and compare the effect of nanoparticles with different modification degrees for protein SERS detection:

[0046] Take 1.5mL of silver nanoparticle sol, centrifuge at room temperature (5000r / min, 10min), remove the supernatant, then add ultrapure water to make the volume of nanoparticle sol to 1.5mL, and detect the zeta potential value of the sol. Continuously add 5 μL of 1 mM KI solution to the sol, equilibrate at 25° C. for 10 min after each addition, and then detect the zeta potential value of the sol. Each sample point was measured 5 times continuously and the average value was taken.

[0047] Take 4.5mL silver nanoparticle sol, centrifuge at room temperature (5000r / min, 10min), remove the supernatant, and concentrate the nanoparticle sol to about 50μL. Add 50 μL of KI solutions of different concentrations, mix well, let stand at 25°C for 20 minutes, then fully mix with 50 μL of 1 mg / mL lysozyme protein solu...

Embodiment 3

[0051] Using the method of anion-modified nanoparticles to enhance Raman spectroscopy to study protein molecules, taking lysozyme as an example, comparing the conventional Raman of lysozyme molecules, SERS on anion-modified nanoparticles and SERS on unmodified nanoparticles:

[0052] Iodide ion-modified silver nanoparticles and unmodified silver nanoparticles were synthesized, mixed with 50 μL of 1 mg / mL lysozyme protein solution, and transferred to a 96-well plate for surface-enhanced Raman testing. Another appropriate amount of 100 mg / mL lysozyme solution was taken in a 96-well plate for conventional Raman testing.

[0053] Figure 4 It is the experimental result of embodiment 3. Figure 4 The middle curve a represents the conventional Raman spectrum of lysozyme solution, and curve b represents the SERS spectrum of lysozyme on iodide ion-modified silver nanoparticles. Comparing a and b, it can be found that the SERS spectrum of lysozyme on anion-modified nanoparticles The ...

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Abstract

Relating to laser Raman spectrum detection, the invention discloses a method for enhancing Raman spectroscopy by anion modified nanoparticles. According to the method, metal nanoparticles are prepared into a core, a full layer is strongly adsorbed with surface modified nanoparticles with anions as a modification layer; a nanoparticle sol and a to-be-measured biomolecule are mixed uniformly; and surface-enhanced Raman spectroscopy detection is conducted directly. The method for enhancing Raman spectroscopy by anion modified nanoparticles provided by the invention has the advantages of simple operation, fast speed, low cost, high sensitivity, good reproducibility, effective maintaining of the native conformation of biomolecules, and accurate and reliable result, thus being suitable for biomolecule study.

Description

technical field [0001] The invention relates to the detection of laser Raman spectrum, in particular to a method for enhancing Raman spectrum with anion-modified nanoparticles. Background technique [0002] Surface-enhanced Raman Spectroscopy (SERS) is a spectroscopic technique with extremely high surface sensitivity, which can identify species adsorbed on the surface of nanostructures through the vibrational characteristics of molecules. Therefore, SERS has received more and more attention in the fields of physics, chemistry, surface science, nanoscience and biomedicine. Among them, biomedicine is one of the most concerned application fields, because surface-enhanced Raman spectroscopy can not only achieve high-sensitivity detection of low-concentration biological samples, but also obtain spectral information of tissues or cells under close to physiological conditions. level to explain life-related phenomena. However, poor reproducibility and low sensitivity are often enc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
Inventor 任斌许丽佳宗铖郑晓姗胡佩
Owner XIAMEN PUSHI NANO TECH CO LTD
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