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Tea polysaccharide derivative and preparation method thereof

A technology for tea polysaccharides and derivatives, which is applied in the field of tea polysaccharide derivatives and their preparation, can solve problems such as the preparation methods of tea polysaccharide derivatives that have not yet been seen, and achieve the effects of cheap equipment and raw materials, convenient operation, and maintaining biological activity

Inactive Publication Date: 2014-03-05
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there is no report on the preparation method of tea polysaccharide derivatives containing tertiary amino groups

Method used

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  • Tea polysaccharide derivative and preparation method thereof
  • Tea polysaccharide derivative and preparation method thereof
  • Tea polysaccharide derivative and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) Weigh 0.5 g of tea polysaccharide, put it in 60 ml of acetic acid-sodium acetate buffer solution (pH 4.0), and stir at 20°C for 20 hours to dissolve.

[0040] (2) Weigh 5 g of EDC, put it in 8 ml of acetic acid-sodium acetate buffer (pH 4.0), stir at 20°C for 8 minutes to dissolve.

[0041] (3) Add the mixed solution obtained in step (2) dropwise to the mixed solution obtained in step (1), react at 20°C for 10 hours, put the reaction solution into a dialysis bag (molecular weight cut-off 1,000), and dialyze against distilled water for 3 days , and the intermediate product was obtained after freeze-drying, which was named NACU-TPS. The structural analysis was carried out by infrared spectroscopy and nuclear magnetic resonance spectroscopy experiments, and the degree of substitution was calculated to be 0.3.

[0042] (4) Weigh 0.3 g of the intermediate product NACU-TPS obtained in step (3), dissolve it in 30 ml of anhydrous dimethyl sulfoxide, and stir at 20°C for 24 ...

Embodiment 2

[0052] (1) Weigh 1 gram of tea polysaccharide, put it in 100 ml of citric acid-sodium citrate buffer (pH 4.4), stir at 25°C for 10 hours to dissolve.

[0053] (2) Weigh 3 g of EDC, put it in 5 ml of citric acid-sodium citrate buffer (pH 4.4), and stir at 25°C for 20 minutes to dissolve.

[0054] (3) Add the mixed solution obtained in step (2) dropwise to the mixed solution obtained in step (1), react at 25°C for 20 hours, put the reaction solution into a dialysis bag (molecular weight cut-off 500), and dialyze against distilled water for 2 days , and the intermediate product was obtained after freeze-drying, which was named NACU-TPS. The structural analysis was carried out by infrared spectroscopy and nuclear magnetic resonance spectroscopy experiments, and the degree of substitution was calculated to be 0.3.

[0055] (4) Weigh 0.5 g of the intermediate product NACU-TPS obtained in step (3), dissolve it in 25 ml of anhydrous dimethyl sulfoxide, and stir at 25°C for 10 hours to...

Embodiment 3

[0059] (1) Weigh 0.1 g of tea polysaccharide, put it in 10 ml of disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution (pH5.8), stir at 15°C for 24 hours to dissolve.

[0060] (2) Weigh 1 gram of EDC, put it in 3 ml of disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution (pH5.8), and stir at 15°C for 30 minutes to dissolve.

[0061] (3) Add the mixed solution obtained in step (2) dropwise to the mixed solution obtained in step (1), react at 15°C for 24 hours, put the reaction solution into a dialysis bag (molecular weight cut-off 2,000), and dialyze against distilled water for 5 days , and the intermediate product was obtained after freeze-drying, which was named NACU-TPS. The structural analysis was carried out by infrared spectroscopy and nuclear magnetic resonance spectroscopy experiments, and the degree of substitution was calculated to be 0.1.

[0062] (4) Weigh 0.1 g of the intermediate product NACU-TPS obtained in step (3), dissolve ...

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Abstract

The invention discloses a method for preparing a tea polysaccharide derivative. The method uses tea polysaccharide with a hypoglycemic activity as a raw material to synthesize the tea polysaccharide derivative containing a tertiary amine group through a chemical coupling reaction at room temperature. According to the method, the reaction condition is mild, usage of a strong acid solvent is avoided, and degradation of the tea polysaccharide is avoided to a great extent, and the bioactivity of the tea polysaccharide can be maintained. The method is simple in process, convenient to operate, and low in cost of equipment and raw materials. The tea polysaccharide derivative containing the tertiary amine group prepared by the method is expected to be used as a genetic vector to be used in a liver cell transfected insulin gene, and the hypoglycemic performance can be further improved, so that the tea polysaccharide derivative has a certain application prospect in the field of diabetes mellitus treatment.

Description

technical field [0001] The invention belongs to the field of chemical modification of natural polysaccharides, and in particular relates to a tea polysaccharide derivative and a preparation method thereof. Background technique [0002] Polysaccharides are derived from nature, which are not only safe and non-toxic, but also have good biocompatibility and degradability. Through chemical reactions, some polysaccharide derivatives containing amine groups (such as chitosan derivatives) can be synthesized as gene carriers, which can efficiently and safely introduce genes into cells to treat diseases (Lu B., et al. Journal of Controlled Release 2009. 137, 54–62). [0003] A large number of studies have shown that tea polysaccharides have obvious biological activities such as lowering blood sugar (Wang Dongfeng et al., Chinese Herbal Medicine, 1995, 26(5), 255-257; Xu Zhongxi et al., Tea Science. 2004, 24(2), 75-81; Fu Hai Ping, Tea Communication. 2006, 33(2), 24-28). The invent...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K48/00A61P3/10
Inventor 杨立群伍淑韵梁玄邹珊珊潘靖雯吴燕琳张黎明
Owner SUN YAT SEN UNIV
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