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Method for preparing thermosensitive antimicrobial film and implant material from antibacterial composition

A technology of antibacterial composition and implant material, applied in textiles and papermaking, medical science, non-woven fabrics, etc., can solve the problems of poor pertinence, microbial or bacterial drug resistance, etc., and achieve the effect of guaranteeing the treatment of infection

Active Publication Date: 2015-04-22
WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Postoperative infection is mostly exogenous, and then induces endogenous. When high fever occurs, systemic anti-infection treatment is a common clinical method, which is not very targeted. Large-dose injections can easily lead to drug resistance of microorganisms or bacteria sex

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The present embodiment prepares thermosensitive antibacterial film according to the following method:

[0035] (1) Prepare PLGA polymer solution: dissolve 10g PLGA (Mw=80,000, LA / GA=75 / 25) in 40mL THF and acetone mixed solvent (the volume ratio of THF to acetone is 1 / 1), and prepare the concentration As a 25% (W / V) solution, stir at room temperature for 3 hours.

[0036] (2) Preparation of PNIPAM colloid: Dissolve 4g of N-isopropylacrylamide, 0.06g of potassium persulfate and 0.16g of N,N-dimethylacrylamide in 160g of deionized water containing 0.3g of gelatin, and heat to 75°C for stability , add 2g vancomycin, continue heating under nitrogen protection for 2 hours, then centrifuge to separate the PNIPAM colloid containing vancomycin, and wash with deionized water three times-centrifugation to obtain cross-linked PNIPAM colloid. The prepared PNIPAM colloid has a uniform particle size and a diameter of 50nm to 500nm, and the loading rate of vancomycin (relative to the ...

Embodiment 2

[0041] The present embodiment prepares thermosensitive antibacterial film according to the following method:

[0042] (1) Preparation of polylactic acid polymer solution: Dissolve 10g of polylactic acid (Mw=500,000) in 40mL of THF and acetone mixed solvent (the volume ratio of THF to acetone is 1 / 1), and prepare a concentration of 30% (W / V) solution was stirred at room temperature for 5 hours.

[0043] (2) Preparation of PNIPAM colloid: Dissolve 10g of N-isopropylacrylamide, 0.2g of potassium persulfate and 0.3g of N,N-dimethylacrylamide in 300g of deionized water containing 0.5g of cyclodextrin, and heat to 90 ℃ stable, add 4g levofloxacin, continue heating under nitrogen protection for 2 hours, then centrifuge to separate the PNIPAM colloid containing levofloxacin, and wash with deionized water three times-centrifugation to obtain cross-linked PNIPAM colloid. The prepared PNIPAM colloid has a uniform particle size and a diameter of 100nm to 600nm, and the loading rate of l...

Embodiment 3

[0048] The present embodiment prepares thermosensitive antibacterial film according to the following method:

[0049] (1) Preparation of polycaprolactone polymer solution: Dissolve 8g of polycaprolactone (Mw=50,000) in 50mL of THF and acetone mixed solvent (the volume ratio of THF to acetone is 1 / 1), and prepare a concentration of 5 % (W / V) solution, stirred at room temperature for 2 hours.

[0050] (2) Preparation of PNIPAM colloid: Dissolve 5g of N-isopropylacrylamide, 0.1g of potassium persulfate and 0.2g of N,N-dimethylacrylamide in 200g of deionized water containing 0.4g of gelatin, and heat to 60°C for stability , add 2.5g cephalexin, continue heating under nitrogen protection for 2 hours, then centrifuge the PNIPAM colloid containing cephalexin, and wash with deionized water three times-centrifugation to obtain cross-linked PNIPAM colloid. The prepared PNIPAM colloid has a uniform particle size and a diameter of 50nm to 300nm, and the loading rate of cephalexin after w...

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Abstract

The invention discloses a method for preparing a thermosensitive antimicrobial film and an implant material from an antibacterial composition. The method comprises the following steps: injecting the antibacterial composition into an injector, and additionally arranging a stainless steel syringe needle; coating the implant material to be coated by adopting 10-30KV of high-voltage power supply, at 1-5mL / h of flow rate of a solution and 5-25cm of receiving distance; and finally drying the coated implant material in vacuum at room temperature for 24-48 hours, so as to obtain the thermosensitive antimicrobial film coated with the implant material, wherein the antibacterial composition comprises an organic polymer selected from polylactic acid, polycaprolactone, a polylactic acid-glycolic acid polymer and a polylactic acid-polyethylene glycol segmented copolymer, blending colloid of isopropylacrylamide and cyclodextrin or blending colloid of isopropylacrylamide and gelatin, and an antibacterial agent. The thermosensitive antimicrobial film prepared by the method can release antimicrobial medicines as long as the temperature of a human body ascends. Therefore, the method has strong pertinence.

Description

technical field [0001] The invention relates to the technical field of antibacterial implant materials, in particular to a method for preparing a temperature-sensitive antibacterial film and an implant material by using an antibacterial composition. Background technique [0002] Fever is a typical reaction when organisms are infected by pathogens. Homeotherms, such as mice or humans, increase their body temperature to signal infection; while cold-blooded animals (poikiotherms, such as nematodes) swim to warmer environments , indicating that it is infected. And when the temperature in the body or the environment rises, the immune signal transmission pathway of the temperature-sensitive sensory factor (HSF-1) will be activated, which can explain that the function of the HSF-1 pathway is to resist the infection of foreign enemies, and it can also explain Why organisms may have a fever when they are infected. From the perspective of physiological response, fever is caused by t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L31/10A61L31/16D04H1/4382D04H1/728
Inventor 韩志超许杉杉
Owner WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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