Wheat germ protein source zinc phytochelatin and preparation method thereof

A wheat germ protein and chelating peptide technology, which is applied in the preparation methods of peptides, chemical instruments and methods, peptides, etc., to achieve the effects of high separation efficiency, clear purpose and small workload

Active Publication Date: 2014-03-12
JIANGNAN UNIV
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  • Wheat germ protein source zinc phytochelatin and preparation method thereof
  • Wheat germ protein source zinc phytochelatin and preparation method thereof
  • Wheat germ protein source zinc phytochelatin and preparation method thereof

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Embodiment Construction

[0028] As previously mentioned, in view of the deficiencies in the prior art, one aspect of the present invention aims to provide a kind of wheat germ protein source zinc chelating peptide, which comprises SEQ ID No.1 and / or SEQ ID No.2 shown The amino acid sequence has a strong metal chelation effect on zinc and other trace elements, and the bioavailability of the peptide-zinc chelate formed with zinc is higher than that of inorganic zinc, which is easier to digest and absorb, and can meet the body's demand for protein.

[0029] Another aspect of the present invention aims to provide a preparation method of wheat germ protein source zinc chelating peptide. In a typical embodiment of the present invention, the preparation method may include the following steps:

[0030] (i) Protein extraction step:

[0031] Weigh a certain quality of defatted wheat germ and dissolve it in deionized water (10 w / v %-20w / v %), adjust the pH to 8.5-9.5 with 1 mol / L NaoH solution, and stir in an u...

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Abstract

The invention discloses wheat germ protein source zinc phytochelatin and a preparation method thereof. The zinc phytochelatin comprises an amino acid sequence as shown by SEQIDNo.1 and/or SEQIDNo.2. The preparation method comprises the following steps: with degreased wheat germ as a raw material, carrying out protein extraction and enzymatic hydrolysis to obtain zymolyte containing zinc phytochelatin; and then, screening and separating out sequentially to obtain the target product, namely the wheat germ protein source zinc phytochelatin through immobilized zinc ion affinity chromatography, macroporous adsorption resin and reversed-phase high-performance liquid chromatography. The zinc phytochelatin metal has strong chelation, can be used as a carrier for transporting trace elements such as zinc, and improves absorption of the trace elements; particularly, after the phytochelatin and zinc form peptide-zinc chelate, the bioavailability of the peptide-zinc chelate is higher than that of inorganic zinc, the peptide-zinc chelate is easier to digest and absorb, and meets the requirement of an organism on protein. The preparation method is simple and easy to realize large-scale implementation, and the raw materials are low in cost.

Description

technical field [0001] The invention particularly relates to a wheat germ protein source zinc chelating peptide and a preparation method thereof, belonging to the technical field of wheat intensive processing. Background technique [0002] Zinc is an indispensable trace element in the human body. Zinc deficiency can lead to poor growth and development, mental retardation, and decreased immunity in children. Traditional zinc supplements are inorganic salts and simple organic acid salts, such as zinc sulfate, zinc gluconate, zinc licorice, zinc citrate, etc. However, studies have shown that they have poor stability, low absorption and utilization, and are irritating to the stomach and intestines, so they cannot be taken for a long time. In recent years, metal chelating peptides have received extensive attention from scholars at home and abroad. Zinc can form chelated salts with metal chelating peptides, and can promote the absorption of trace elements in the form of chelated...

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Application Information

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IPC IPC(8): C07K7/06C07K7/08C12P21/06C07K1/22C07K1/20
Inventor 朱科学王晓萍郭晓娜宋珊珊周惠明彭伟
Owner JIANGNAN UNIV
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