Composite microecological preparation for broiler chickens
A technology of compound microecology and preparation, applied in application, animal feed, animal feed, etc., can solve the problems of low content of beneficial live bacteria, poor stability, and single efficacy, so as to reduce costs, improve economic benefits, and improve feed conversion and utilization rate effect
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Embodiment 1
[0012] (1) Activation of various strains, composition of shake flask culture medium and shake flask culture conditions
[0013] Rhodopseudomonas palustris activation and shake flask medium composition: NH 4 Cl1.0g, CH 3 COONa3.5g, MgCl 2 0.1g, CaCl 2 0.1g, KH 2 PO 4 0.6g, K 2 HPO 4 0.4g, yeast extract 0.1g, water 1000ml, pH7.5. Shake flask culture conditions: temperature 30°C, 72 hours; light 3000lx;
[0014] Bacillus subtilis activation and shake flask medium composition: 5 grams of beef extract, 5 grams of peptone, 5 grams of NaCl, 1 gram of yeast powder, 0.2 grams of calcium carbonate, MgSO 4 0.5 grams, 1000ml of tap water, PH=7.0-7.5; shake flask culture conditions: temperature 35°C, 220 rpm, 24 hours;
[0015] Lactobacillus acidophilus activation and shake flask medium composition: MRS modified medium (peptone 10g, yeast extract 5g, beef extract 10g, glucose 20g, sodium acetate 5g, sodium citrate 2.15g, soil temperature 801ml, MgSO 4 ·7H 2 O 0.58 g, MnSO 4 4H ...
Embodiment 2
[0026] The medium and culture conditions of each strain are the same as above. After activation, shake flask culture, primary expansion, and secondary expansion culture of each bacterial strain, the ratio of the number of bacteria is as follows: Rhodopseudomonas palustris: Bacillus subtilis: acidophilus Lactobacillus: Candida genus: Bacillus natto = 4: 12: 10: 4: 2 for mixing, then add adsorbent to the mixed fermentation broth for adsorption and drying to obtain the product. The composition and weight ratio of the adsorbent are rice bran:wheat bran:soybean meal=1:1:1.
[0027] The beneficial effects of the compound probiotics for broiler chickens of the present invention will be further described in conjunction with the efficacy experiment on broiler chickens.
[0028] 1 Experimental materials and methods
[0029] 1.1 Experimental animals and grouping: 600 1-day-old commercial generation AA white-feather broilers (half male and half male) were selected and divided into 3 grou...
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