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Method for extracting sodium copper chlorophyll from silkworm excrement

A technology of chlorophyll copper sodium salt and silkworm sand, which is applied in the direction of organic chemistry, etc., can solve the problems of increasing the difficulty of chlorophyll extraction, increasing cost and time consumption, increasing environmental pollution, etc. economic effect

Active Publication Date: 2014-03-19
YUNNAN RAINBOW BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, because silkworm excrement contains a large amount of macromolecular substances such as cellulose, hemicellulose, pectin, and protein, it increases the difficulty of extracting chlorophyll, and generally requires a large amount of solvents to complete the extraction of silkworm excrement raw materials, which increases production. cost and time consumption, and because the solvent is acetone, the large use of acetone also increases the pollution to the environment, which is not conducive to environmental protection

Method used

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  • Method for extracting sodium copper chlorophyll from silkworm excrement
  • Method for extracting sodium copper chlorophyll from silkworm excrement
  • Method for extracting sodium copper chlorophyll from silkworm excrement

Examples

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Effect test

Embodiment 1

[0080] Present embodiment realizes with the silkworm excrement raw material of 5000g first silkworm age stage.

[0081] Precisely weigh 3.10g of cellulase compound enzyme preparation, 2.78g of hemicellulase compound enzyme preparation, 1.81g of pectinase compound enzyme preparation, and 0.46g of triacylglycerol acyl hydrolase, dissolve them in 3000g of 40°C water, and spray them on Into 5000g of crushed silkworm excrement powder, spray while stirring and mixing. After 1 hour, dissolve 2.13g of protease in water and spray it on the raw material. After stirring evenly, make it hydrolyze for 5 hours.

[0082] Enzymatic hydrolysis: put the silkworm excrement hydrolyzed for 5 hours in a constant temperature environment of 30°C for 6 hours, and stir in time during the reaction, so that the enzymatic hydrolysis reaction can fully proceed.

[0083]Granulation: Re-granulation is carried out after the reaction is completed. First, the muddy silkworm excrement with a water content of 65%...

Embodiment 2

[0088] Present embodiment realizes with the silkworm excrement raw material of 5000g second silkworm age stage.

[0089] Precisely weigh 4.82g of the pectinase compound enzyme preparation and 3.37g of the cellulase compound enzyme preparation and dissolve them in 3000g of 40°C water. After fully dissolving, spray them into 5000g of crushed silkworm excrement powder, and stir and mix evenly while spraying; 4.15g was dissolved in water, sprayed on the raw material and stirred for 3h.

[0090] Enzymatic hydrolysis: put the silkworm sand mud added with enzymes in a constant temperature environment of 30°C for 6 hours, and stir in time during the reaction, so that the enzymatic hydrolysis reaction can fully proceed.

[0091] Granulation: Re-granulation is carried out after the reaction is completed. First, the muddy silkworm excrement with a water content of 65% by weight is dried at a high speed for 5 minutes at 105°C until the water content of the silkworm excrement powder is 40%...

Embodiment 3

[0096] Present embodiment realizes with the silkworm excrement raw material of 5000g the third silkworm age stage.

[0097] Accurately weigh 3.10g of the cellulase compound enzyme preparation, 2.78g of the hemicellulase compound enzyme preparation, and 1.81g of the pectinase compound enzyme preparation and add them to 3000g of water. Stir to mix while spraying.

[0098] Enzymatic hydrolysis: put the silkworm sand mud added with enzymes in a constant temperature environment of 30°C for 6 hours, and stir in time during the reaction, so that the enzymatic hydrolysis reaction can fully proceed.

[0099] Granulation: Re-granulation is carried out after the reaction is completed. First, the muddy silkworm excrement with a water content of 65% by weight is dried at a high speed for 5 minutes at 105°C until the water content of the silkworm excrement powder is 40% by weight, and then high-speed granulation is used. Machine into particles with a diameter of 0.5 cm and a length of 1.5 ...

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Abstract

The invention discloses a method for extracting sodium copper chlorophyll from silkworm excrement. Specifically, the method includes enzymolysis of silkworm excrement by enzyme, wherein the enzyme is one or more of scellulase, hemicellulase, pectinase, triacylglycerol acylhydrolase and protease. The method provided by the invention can effectively degrade cellulose, hemicellulose, pectin, protein and fat components in the silkworm excrement raw material. Also, the method has the advantages of small enzyme dosage, simple treatment process, easy operation, short time, high chlorophyll extraction rate, high purity sodium copper chlorophyll finished product, and no environmental pollution, improves the yield of sodium copper chlorophyllin, shortens the extraction time of chlorophyll to a large extent, reduces the dosage of the solvent, reduces difficulties during subsequent preparation of sodium copper chlorophyll, and saves the production cost.

Description

technical field [0001] The invention relates to a method for extracting copper chlorophyll sodium salt from silkworm sand. Background technique [0002] Chlorophyll is extracted from silkworm sand and then through a series of reactions, copper chlorophyll sodium salt can be prepared. As a kind of metalloporphyrin, compared with chlorophyll, it not only has enhanced stability, but also has good water solubility and is easy to use. Moreover, copper chlorophyll sodium salt has unique advantages in coloring, improving or maintaining natural green food coloring and improving the use value of food itself due to its unique structural characteristics and the nutrients chelated in derivatives, so it is widely used. Used in food additives, cosmetic additives, colorants, pharmaceuticals, photoelectric conversion materials and other fields. As a safe food additive and coloring agent, copper chlorophyll sodium salt can be used in soft drinks, candy, fruit powder, sherbet, prepared wine,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D487/22
CPCC07D487/22
Inventor 罗立梅代刚王晓云李磊欧敏功杨巡纭
Owner YUNNAN RAINBOW BIO TECH
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