High-efficiency biological reactor and dioscin high-efficiency biological hydrolysis method

A technology of bioreactor and diosgenin, which is applied in the direction of bioreactor/fermenter combination, stress-stimulated microbial growth method, specific-purpose bioreactor/fermenter, etc., can solve the problem of uneven magnetic field and no Scientifically used in bioreactor equipment and technology to achieve the effect of improving biological reaction efficiency, low cost, and low energy consumption

Inactive Publication Date: 2014-12-17
HUBEI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, studies have found that magnetic fields can significantly promote the growth and metabolism of biological cells. Magnetic fields are considered to have a significant regulatory effect on cell growth and secondary metabolism, but how to scientifically use magnetic fields to promote the growth of cultured cells in biotechnology Metabolism is still an unsolved technical problem, especially in large-scale bioreactors, the use of a simple external magnetic field will inevitably lead to uneven magnetization of the cultured cells in the reactor; although the cultured cells can be achieved by stirring the external magnetic field, but However, this requirement cannot be achieved for bioreactors for immobilized culture. How to combine magnetic field and immobilized culture science is also a difficult problem in biotechnology; at the same time, in bioreactors, immobilized cell culture and maximize Increasing the reaction area can improve the reaction efficiency, how to increase the reaction area is also a technical problem, and the present invention provides the technical
The equipment and technology to apply these new findings scientifically to bioreactors are not currently available

Method used

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  • High-efficiency biological reactor and dioscin high-efficiency biological hydrolysis method
  • High-efficiency biological reactor and dioscin high-efficiency biological hydrolysis method
  • High-efficiency biological reactor and dioscin high-efficiency biological hydrolysis method

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Experimental program
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Effect test

Embodiment 1

[0044] A high-efficiency bioreactor, comprising a fermenter 1, an air intake system, a circulation pipe 15 outside the fermenter, an air outlet pipe 26 at the top of the fermenter 1, an inlet and outlet pipe 25 at the bottom of the fermenter 1, and an air outlet 21 at the top of the fermenter 1 And the inlet and outlet 23 of bottom, described air outlet 21 is at the upper end of air outlet pipeline, and described inlet and outlet 23 is at the lower end of inlet and outlet pipeline 25, and described air intake system comprises air inlet pipeline 24, high-pressure air pump 11, air inlet sterile filter 12 and the air inlet valve 14, the air inlet pipeline 24, the inlet and outlet pipeline 25, and the circulation pipeline 15 are connected. The circulation pipeline 15 is provided with a sampling port 22, and the sampling port 22 is provided with a sampling port valve 6. The entrance and exit 23 is provided with an entrance and exit valve 8;

[0045] An aerobic electrode 17 and a pH...

Embodiment 2

[0066] On the basis of the high-efficiency bioreactor of embodiment 1, the structure is embodied, and the dioscin biohydrolysis test is carried out:

[0067] In the fermenter 1 with a volume of 5 liters, the magnetic beads 2 are filled to 1 / 2 of the height of the tank. The magnetic beads 2 used in this experiment are solid magnetic beads 19 with a rough surface of polytetrafluoroethylene. The solid magnetic beads 19 The surface of the first outer cladding layer 19-1 is evenly distributed with thorn-like protrusions, the length of the protrusions is 0.5 cm, the outer diameter of the magnetic beads is 1.0 cm, and the magnetic field strength on the surface of the solid magnetic beads 19 is 120 mT, and then the inlet and outlet 23 are connected to the air heater of the gas heating device 9 connection, open the air heater 9 and the blower 10 to blast hot air at 300°C into the tank, sterilize it for 2 hours, then inject the LB culture solution with rhamnose as the sugar source, and i...

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Abstract

The invention belongs to the technical field of biological reactors, and particularly discloses a high-efficiency biological reactor and a dioscin high-efficiency biological hydrolysis method. The high-efficiency biological reactor is characterized in that magnetic beads are filled or suspended in a fermentation tank; an air inlet pipeline is provided with a negative ion generator; the negative ion generator, an oxygen electrode and a pH meter are connected with a master controller; the master controller regulates the ventilation work of the negative ion generator according to data output by the oxygen electrode and the pH meter; a magnetizer is wrapped on a circulating pipeline. The high-efficiency biological reactor disclosed by the invention can outstandingly enhance the cell reaction activity by scientifically combining the immobilized culture and magnetic field technology of a biological reactor and is applied to dioscin biological hydrolysis reaction. Compared with the traditional acid hydrolysis reaction, the dioscin high-efficiency biological hydrolysis method has the advantages of no pollution, low cost and low energy consumption.

Description

technical field [0001] The invention relates to the technical field of bioreactors, in particular to a high-efficiency bioreactor and a high-efficiency biohydrolysis method for diosgenin or other biomedicines such as Penicillium fermentation to produce penicillin, Artemisia annua cells to produce artemisinin and the like. Background technique [0002] A bioreactor is a general term for cultivating living cells or tissues or living organisms in a container that can control the culture conditions to produce biologically active substances or tissues or individuals needed by humans. It has a wide range of applications in alcohol, biomedicine, food production, and environmental governance. The control conditions of bioreactors currently used by humans include: medium formula, dissolved oxygen rate, pH, osmotic potential, temperature, light, etc. In recent years, studies have found that magnetic fields can significantly promote the growth and metabolism of biological cells. Magne...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/36C12M1/34C12M1/04C12P33/20C12R1/80
CPCC12M27/00C12M25/16C12M35/06
Inventor 董静洲朱蕾艾训儒郑小江姚兰雷灿
Owner HUBEI UNIV FOR NATITIES
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