Mixed neutral cellulase and its preparation method and application in papermaking beating

A neutral cellulase, cellulase technology, applied in textiles and papermaking, biochemical equipment and methods, cellulose treatment with microorganisms/enzymes, etc., can solve problems such as large waste discharge and achieve a wide range of effects , strong thermal stability, the effect of reducing power consumption

Inactive Publication Date: 2016-08-17
宁夏夏盛实业集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The technical problem solved by the present invention is to overcome the defect of large waste discharge in the existing pulping and papermaking technology, and use a mixed neutral cellulase to decompose the inside of the cellulose to promote the separation of fibers in the pulping process Blending makes beating easier, thereby saving beating energy consumption, and improving the white water circulation rate, thereby reducing the consumption of raw pulp per ton of paper and the amount of clean water, and improving the quality of sewage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The preservation number of the Aspergillus niger strain provided by the present invention is CICC2475, which is purchased from the China Industrial Microorganism Culture Collection and Management Center. The Aspergillus niger slant culture medium is composed of: 200 g of potato boiled juice, 20 g of glucose, 20 g of agar, 1000 mL of distilled water, pH 5.8, sterilized at 121° C. for 20 min;

[0050] Bacillus subtilis CICC10158 was purchased from China Industrial Microorganism Collection and Management Center. The Bacillus subtilis slant culture medium is composed of: beef extract 3g, sodium chloride 5g, peptone 10g, agar 15g, distilled water 1000mL, pH value 6.8, sterilization at 121°C for 20min;

[0051] The Aspergillus niger primary, secondary and tertiary seed culture medium is composed of: 200 g of potato boiled juice, 20 g of glucose, 10-30 g of trehalose, 1000 mL of distilled water, pH 5.5, sterilized at 121° C. for 20 min;

[0052] The Aspergillus niger primary ...

Embodiment 2

[0061] The composition of the mixed neutral cellulase in parts by weight is as follows: 30 parts of cellulase, 25 parts of xylanase, 8 parts of pectinase, 5 parts of mannanase, and 5 parts of Chinese herbal medicine powder.

[0062] The preparation method of the Chinese herbal medicine powder is as follows: weighing 24 parts of Angelica sinensis; 14 parts of Codonopsis pilosula; 13 parts of Bupleurum Radix; 14 parts of Scutellaria baicalensis; respectively crushing the above Chinese herbal medicines to a particle size of less than 2 mm, then uniformly mixing in a container and adding 4 times the weight The water was kept at 90 °C for 4 hours, the mixture of ethanol and propanol was added with 3 times the weight of the mixture, the temperature was controlled to 60 °C for 4 hours, and filtered; the filtrate was vacuum concentrated and freeze-dried to obtain Chinese herbal medicine powder.

[0063] Described acid xylanase is prepared by following technical scheme:

[0064] (1) Ac...

Embodiment 3

[0106] The usage and dosage of the mixed neutral cellulase:

[0107] According to the different pulp raw materials, processes and equipment, the appropriate products are selected, and the addition methods of biological enzymes are also different, which can be mainly divided into the following two categories:

[0108] (1) Addition location of the production line where the paper mill makes its own pulp and directly supplies pulp: the possible position between the pulp outlet of the last pulp washer and the pulp inlet of the knock-out tank; the pulp obtains a suitable temperature and PH value after pulp washing , knocking the front pool can make the biological enzymes get enough residence time.

[0109] Addition method: According to the slurry feeding method and flow rate, the biological enzyme is formulated into a solution that is easy and accurate to operate, and then added to the slurry synchronously with a metering pump.

[0110] (2) Addition location of production line usin...

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Abstract

The invention discloses a mixed neutral cellulase, and belongs to the field of enzymatic preparations. The mixed neutral cellulase comprises, in parts by weight, 10-40 parts of cellulase, 20-30 parts of acidic xylanase, 5-10 parts of pectase, 3-8 parts of mannanase and 2-8 parts of a Chinese herbal medicine powder. According to the prepared mixed neutral cellulase, the active components of the Chinese herbal medicine powder is effectively utilized, a special method is utilized to overcome the disadvantage that conventional acidic xylanase is fermented by employing a single bacterial strain; compared with unprocessed pulp, beat pulp processed by the mixed neutral cellulase is improved in beating degree by 11.76%, improved in short fiber burst index by 11.98% and improved in ring crush index by 13.99%; the beat pulp processed by the mixed neutral cellulase is improved in long fiber pulling force index by 40.17% and improved in tear index by 58.91%; the beat pulp processed by the mixed neutral cellulase has relatively smooth fiber surface and relatively soft enzyme fiber; paper made from the beat pulp processed by the mixed neutral cellulase is improved in tear index by 8.3%, improved in tensile index by 10.6% and improved in burst index by 16.5%; and paper pulp whiteness is improved by 2%-5%, sewage quality is substantially improved, and environmental pollution degree is reduced.

Description

Technical field: [0001] The invention belongs to the field of enzyme preparations, and particularly relates to enzymes used in the field of pulp and paper making. Background technique: [0002] The pulp and paper industry is one of the important pillar industries of the national economy, but it is also a major source of resource consumption and environmental pollution such as forests, energy, and chemicals. The global paper industry cuts down hundreds of millions of cubic meters of forest every year, and about half of it is turned into waste and discharged back into the surrounding atmosphere and water, posing a huge threat and harm to the ecological environment of human existence. Reducing energy and chemical consumption, improving pulp yield, and biological treatment of sewage are all fundamental ways to overcome the above difficulties. And biotechnology is precisely in these areas can make a big difference. [0003] Cellulase is a complex enzyme system composed of a var...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/42C12N9/88C12N9/24D21C5/00
CPCC12N9/18C12N9/2437C12N9/248C12N9/2488C12N9/88D21C5/005
Inventor 赵迎春王刚谢合群
Owner 宁夏夏盛实业集团有限公司
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