Mixed neutral cellulase and its preparation method and application in papermaking beating
A neutral cellulase, cellulase technology, applied in textiles and papermaking, biochemical equipment and methods, cellulose treatment with microorganisms/enzymes, etc., can solve problems such as large waste discharge and achieve a wide range of effects , strong thermal stability, the effect of reducing power consumption
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Embodiment 1
[0049] The preservation number of the Aspergillus niger strain provided by the present invention is CICC2475, which is purchased from the China Industrial Microorganism Culture Collection and Management Center. The Aspergillus niger slant culture medium is composed of: 200 g of potato boiled juice, 20 g of glucose, 20 g of agar, 1000 mL of distilled water, pH 5.8, sterilized at 121° C. for 20 min;
[0050] Bacillus subtilis CICC10158 was purchased from China Industrial Microorganism Collection and Management Center. The Bacillus subtilis slant culture medium is composed of: beef extract 3g, sodium chloride 5g, peptone 10g, agar 15g, distilled water 1000mL, pH value 6.8, sterilization at 121°C for 20min;
[0051] The Aspergillus niger primary, secondary and tertiary seed culture medium is composed of: 200 g of potato boiled juice, 20 g of glucose, 10-30 g of trehalose, 1000 mL of distilled water, pH 5.5, sterilized at 121° C. for 20 min;
[0052] The Aspergillus niger primary ...
Embodiment 2
[0061] The composition of the mixed neutral cellulase in parts by weight is as follows: 30 parts of cellulase, 25 parts of xylanase, 8 parts of pectinase, 5 parts of mannanase, and 5 parts of Chinese herbal medicine powder.
[0062] The preparation method of the Chinese herbal medicine powder is as follows: weighing 24 parts of Angelica sinensis; 14 parts of Codonopsis pilosula; 13 parts of Bupleurum Radix; 14 parts of Scutellaria baicalensis; respectively crushing the above Chinese herbal medicines to a particle size of less than 2 mm, then uniformly mixing in a container and adding 4 times the weight The water was kept at 90 °C for 4 hours, the mixture of ethanol and propanol was added with 3 times the weight of the mixture, the temperature was controlled to 60 °C for 4 hours, and filtered; the filtrate was vacuum concentrated and freeze-dried to obtain Chinese herbal medicine powder.
[0063] Described acid xylanase is prepared by following technical scheme:
[0064] (1) Ac...
Embodiment 3
[0106] The usage and dosage of the mixed neutral cellulase:
[0107] According to the different pulp raw materials, processes and equipment, the appropriate products are selected, and the addition methods of biological enzymes are also different, which can be mainly divided into the following two categories:
[0108] (1) Addition location of the production line where the paper mill makes its own pulp and directly supplies pulp: the possible position between the pulp outlet of the last pulp washer and the pulp inlet of the knock-out tank; the pulp obtains a suitable temperature and PH value after pulp washing , knocking the front pool can make the biological enzymes get enough residence time.
[0109] Addition method: According to the slurry feeding method and flow rate, the biological enzyme is formulated into a solution that is easy and accurate to operate, and then added to the slurry synchronously with a metering pump.
[0110] (2) Addition location of production line usin...
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