Mixed neutral cellulase, preparation method thereof and application thereof to papermaking beating
A technology of neutral cellulase and cellulase, applied in textiles and papermaking, biochemical equipment and methods, using microorganisms/enzymatic cellulose treatment, etc., can solve the problem of large waste discharge, and achieve a wide range of action conditions , high vitality, and the effect of reducing power consumption
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Embodiment 1
[0049] The preservation number of the Aspergillus niger strain provided by the present invention is CICC2475, which was purchased from China Industrial Microorganism Culture Collection and Management Center. The composition of the Aspergillus niger slant medium is: 200g of potato juice, 20g of glucose, 20g of agar, 1000mL of distilled water, pH value 5.8, sterilized at 121°C for 20min;
[0050] Bacillus subtilis CICC10158 was purchased from China Industrial Microorganism Culture Collection Management Center. The composition of the Bacillus subtilis slant medium is: beef extract 3g, sodium chloride 5g, peptone 10g, agar 15g, distilled water 1000mL, pH value 6.8, sterilized at 121°C for 20min;
[0051] The primary, secondary, and tertiary seed culture medium of Aspergillus niger consists of: 200 g of potato boiled juice, 20 g of glucose, 10-30 g of trehalose, 1000 mL of distilled water, pH value of 5.5, and sterilization at 121° C. for 20 min;
[0052] The Aspergillus niger pri...
Embodiment 2
[0061] The composition in parts by weight of the mixed neutral cellulase is as follows: 30 parts of cellulase, 25 parts of xylanase, 8 parts of pectinase, 5 parts of mannanase, and 5 parts of Chinese herbal medicine powder.
[0062] The preparation method of the Chinese herbal medicine powder is as follows: take 24 parts of Angelica sinensis; 14 parts of Codonopsis pilosula; 13 parts of Bupleuri; Control the temperature at 90°C for 4 hours, add a mixture of ethanol and propanol that is 3 times the weight of the mixed material, control the temperature to 60°C for 4 hours, and filter; the filtrate is concentrated in vacuum and then freeze-dried to obtain a Chinese herbal medicine powder.
[0063] Described acid xylanase adopts following technical scheme to prepare:
[0064] (1) Activation of Aspergillus niger and Bacillus subtilis
[0065] The well-preserved Aspergillus niger slant strains were inoculated on the slant medium, cultured at 30°C for 40 hours to activate the strain...
Embodiment 3
[0106] The usage and consumption of described mixed neutral cellulase:
[0107] According to different pulp raw materials, processes, and equipment, suitable products are selected, and the methods of adding biological enzymes are also different, which can be mainly divided into the following two categories:
[0108] (1) Adding location for the production line of the paper mill’s own pulping and direct pulp supply: the possible position between the pulp outlet of the last pulp washer and the pulp inlet of the knocking pool; after pulp washing, the pulp obtains a suitable temperature and pH value , Tapping the pre-pool can allow the biological enzymes to obtain sufficient residence time.
[0109] Adding method: According to the slurry feeding method and flow rate, prepare the biological enzyme into a solution that is easy to operate accurately, and then add it into the slurry synchronously with a metering pump.
[0110] (2) Adding location for the production line using waste pa...
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