Mixed neutral cellulase, preparation method thereof and application thereof to papermaking beating

A technology of neutral cellulase and cellulase, applied in textiles and papermaking, biochemical equipment and methods, using microorganisms/enzymatic cellulose treatment, etc., can solve the problem of large waste discharge, and achieve a wide range of action conditions , high vitality, and the effect of reducing power consumption

Inactive Publication Date: 2014-03-19
宁夏夏盛实业集团有限公司
View PDF8 Cites 18 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The technical problem solved by the present invention is to overcome the defect of large waste discharge in the existing pulping and papermaking technology, and use a mixed neutral cellulase to decompose the inside of the cellulose to promote the separation of fibers in the pulping process Blending makes beating easier, thereby saving beating energy consumption, and improving the white water circulation rate, thereby reducing the consumption of raw pulp per ton of paper and the amount of clean water, and improving the quality of sewage

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The preservation number of the Aspergillus niger strain provided by the present invention is CICC2475, which was purchased from China Industrial Microorganism Culture Collection and Management Center. The composition of the Aspergillus niger slant medium is: 200g of potato juice, 20g of glucose, 20g of agar, 1000mL of distilled water, pH value 5.8, sterilized at 121°C for 20min;

[0050] Bacillus subtilis CICC10158 was purchased from China Industrial Microorganism Culture Collection Management Center. The composition of the Bacillus subtilis slant medium is: beef extract 3g, sodium chloride 5g, peptone 10g, agar 15g, distilled water 1000mL, pH value 6.8, sterilized at 121°C for 20min;

[0051] The primary, secondary, and tertiary seed culture medium of Aspergillus niger consists of: 200 g of potato boiled juice, 20 g of glucose, 10-30 g of trehalose, 1000 mL of distilled water, pH value of 5.5, and sterilization at 121° C. for 20 min;

[0052] The Aspergillus niger pri...

Embodiment 2

[0061] The composition in parts by weight of the mixed neutral cellulase is as follows: 30 parts of cellulase, 25 parts of xylanase, 8 parts of pectinase, 5 parts of mannanase, and 5 parts of Chinese herbal medicine powder.

[0062] The preparation method of the Chinese herbal medicine powder is as follows: take 24 parts of Angelica sinensis; 14 parts of Codonopsis pilosula; 13 parts of Bupleuri; Control the temperature at 90°C for 4 hours, add a mixture of ethanol and propanol that is 3 times the weight of the mixed material, control the temperature to 60°C for 4 hours, and filter; the filtrate is concentrated in vacuum and then freeze-dried to obtain a Chinese herbal medicine powder.

[0063] Described acid xylanase adopts following technical scheme to prepare:

[0064] (1) Activation of Aspergillus niger and Bacillus subtilis

[0065] The well-preserved Aspergillus niger slant strains were inoculated on the slant medium, cultured at 30°C for 40 hours to activate the strain...

Embodiment 3

[0106] The usage and consumption of described mixed neutral cellulase:

[0107] According to different pulp raw materials, processes, and equipment, suitable products are selected, and the methods of adding biological enzymes are also different, which can be mainly divided into the following two categories:

[0108] (1) Adding location for the production line of the paper mill’s own pulping and direct pulp supply: the possible position between the pulp outlet of the last pulp washer and the pulp inlet of the knocking pool; after pulp washing, the pulp obtains a suitable temperature and pH value , Tapping the pre-pool can allow the biological enzymes to obtain sufficient residence time.

[0109] Adding method: According to the slurry feeding method and flow rate, prepare the biological enzyme into a solution that is easy to operate accurately, and then add it into the slurry synchronously with a metering pump.

[0110] (2) Adding location for the production line using waste pa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a mixed neutral cellulase, and belongs to the field of enzymatic preparations. The mixed neutral cellulase comprises, in parts by weight, 10-40 parts of cellulase, 20-30 parts of acidic xylanase, 5-10 parts of pectase, 3-8 parts of mannanase and 2-8 parts of a Chinese herbal medicine powder. According to the prepared mixed neutral cellulase, the active components of the Chinese herbal medicine powder is effectively utilized, a special method is utilized to overcome the disadvantage that conventional acidic xylanase is fermented by employing a single bacterial strain; compared with unprocessed pulp, beat pulp processed by the mixed neutral cellulase is improved in beating degree by 11.76%, improved in short fiber burst index by 11.98% and improved in ring crush index by 13.99%; the beat pulp processed by the mixed neutral cellulase is improved in long fiber pulling force index by 40.17% and improved in tear index by 58.91%; the beat pulp processed by the mixed neutral cellulase has relatively smooth fiber surface and relatively soft enzyme fiber; paper made from the beat pulp processed by the mixed neutral cellulase is improved in tear index by 8.3%, improved in tensile index by 10.6% and improved in burst index by 16.5%; and paper pulp whiteness is improved by 2%-5%, sewage quality is substantially improved, and environmental pollution degree is reduced.

Description

Technical field: [0001] The invention belongs to the field of enzyme preparations, in particular to enzymes used in the field of pulp and papermaking. Background technique: [0002] The pulp and paper industry is one of the important pillar industries of the national economy, but it is also a major contributor to resource consumption and environmental pollution such as forests, energy, and chemicals. The global paper industry cuts down hundreds of millions of cubic meters of trees every year, and about half of them are turned into waste and discharged back into the surrounding atmosphere and water flow, which poses a huge threat and harm to the ecological environment of human existence. Reducing energy and chemical consumption, increasing pulp yield, and biological treatment of sewage are all fundamental ways to overcome the above difficulties. And biotechnology can do a lot in these areas. [0003] Cellulase is a complex enzyme system composed of various hydrolytic enzyme...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/42C12N9/88C12N9/24D21C5/00
CPCC12N9/18C12N9/2437C12N9/248C12N9/2488C12N9/88D21C5/005
Inventor 赵迎春王刚谢合群
Owner 宁夏夏盛实业集团有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap