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A method of converting cellulose into oil by using green algae

A technology of cellulose and oil, applied in the field of microorganisms, can solve the problem of no cellulose being converted into oil, and achieve the effect of strong degradation of cellulose and short growth cycle

Active Publication Date: 2016-05-04
海南古遵科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the main research focuses on the conversion of cellulose to ethanol, and there are very few studies on the conversion of cellulose to oil, especially there is no report on the one-step conversion of cellulose to oil using green algae

Method used

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  • A method of converting cellulose into oil by using green algae

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Experimental program
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Effect test

Embodiment 1

[0014] Example 1. Congo red chromogenic method confirms that A. hainanensis can degrade cellulose under dark conditions

[0015] The medium used is cellulose-MS Congo red medium, and the specific formula is as follows: NH 4 NO 3 1650mgL -l 、KNO 3 1900mgL -l 、KH 2 PO 4 170mgL -l , CaCl 2 2H 2 O440mgL -l , MgSO 4 ·7H 2 O370mgL -l , FeSO 4 ·7H 2 O27.8mgL -l 、Na 2 EDTA37.3gL -l , MnSO 4 4H 2 O22.3mgL -l , ZnSO 4 ·7H 2 O8.6mgL -l 、H 3 BO 3 6.2mgL -l 、KI0.83mgL -l 、Na 2 MoO 4 2H 2 O0.25mgL -l 、CuSO 4 ·5H 2 O0.025mgL -l 、CoCl 2 ·6H 2 O0.0025mgL -l , Inositol 100mgL -l , Glycine 2mgL -l , Thiamine hydrochloride 0.4mgL -l , pyridoxine hydrochloride 0.5mgL -l , Niacin 0.5mgL -l , sucrose 10-30mgL -l , sodium carboxymethyl cellulose 20gL -l , Congo red 200mgL -l , agar powder 9gL -l , pH=6.2.

[0016] The subcultured A. hainanensis was inoculated into the center of the medium plate, and placed in a dark condition for culture at 26°C, and the ...

Embodiment 2

[0017] Embodiment 2. Cultivating Acorns hainanensis in a cellulose-containing liquid medium

[0018] The liquid medium used is MS medium supplemented with shuttlemethyl cellulose, and the specific formula is as follows: NH 4 NO 3 1650mgL -l 、KNO 3 1900mgL -l 、KH 2 PO 4 170mgL -l , CaCl 2 2H 2 O440mgL -l , MgSO 4 ·7H 2 O370mgL -l , FeSO 4 ·7H 2 O27.8mgL -l 、Na 2 EDTA37.3gL -l , MnSO 4 4H 2 O22.3mgL -l , ZnSO 4 ·7H 2 O8.6mgL -l 、H 3 BO 3 6.2mgL -l 、KI0.83mgL -l 、Na 2 MoO 4 2H 2 O0.25mgL -l 、CuSO 4 ·5H 2 O0.025mgL -l 、CoCl 2 ·6H 2 O0.0025mgL -l , Inositol 100mgL -l , Glycine 2mgL -l , Thiamine hydrochloride 0.4mgL -l , pyridoxine hydrochloride 0.5mgL -l , Niacin 0.5mgL -l , glucose or sucrose 30mgL -l Below, sodium carboxymethyl cellulose 20-40gL -l , pH=6.2.

[0019] Inoculate the plate-activated A. hainanensis species in a 500mL Erlenmeyer flask (containing 200mL of the above-mentioned sterilized medium), pre-culture for 10 days at 26...

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Abstract

The invention relates to a method for converting cellulose into grease by using a green alga. The green alga is an unicellular green alga separated from a rubber tree, is named as Heveochlorella hainangensis, and has been preserved in the China General Microbiological Culture Collection Center on January 18, 2013, with the collection number of CGMCC NO: 7101; and the method comprises the following steps: culturing the Heveochlorella hainangensis in a cellulose-containing culture medium in the dark to induce generation of cellulase, and degrading the cellulose to obtain a carbon source so as to grow and propagate and accumulate the grease. The Heveochlorella hainangensis has the characteristics of cultivability, short growth cycle and the like, has relatively strong cellulose degrading ability, and can accumulate the grease, so that a novel way for converting cellulosic materials such as straws into bioenergy is provided and the method has a potential commercial value and an important theoretical practical significance.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a method for converting cellulose into oil by using green algae, in particular to inducing the expression of cellulase gene in green algae, degrading cellulose, and synthesizing oil. Background technique [0002] With the increasing seriousness of mineral energy consumption and ecological environment problems, the research, development and utilization of renewable energy is the general trend of human society and economic development. Biomass energy, as an important part of renewable energy, has attracted more and more attention from all over the world. In 2007, my country issued the "Medium and Long-Term Development Plan for Renewable Energy", which clearly identified biomass energy as a key area of ​​renewable energy development in my country, and strived to achieve 30 million kilowatts of biomass power generation and 44.3 billion cubic meters of biogas in 2020. m, the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12P7/64C12R1/89
Inventor 张家明谭德冠付莉莉孙雪飘
Owner 海南古遵科技有限公司
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