Method for preparing epothilone molecularly imprinted polymer by adopting mixed template

Abstract

The invention discloses a method for preparing an epothilone molecularly imprinted polymer by adopting a mixed template and belongs to the technical field of molecular imprinting. The method comprises the following steps: 1, uniformly mixing epothilone B and epothilone D according to a certain proportion to obtain template molecules, adding a functional monomer and a cross-linking agent into the template molecules, uniformly mixing to form a mixture, adding the mixture into a pore-foaming agent, uniformly mixing under the ultrasonic condition, standing at room temperature, then adding an initiator and uniformly mixing to obtain mixed liquid; 2, performing the polymerization reaction on the mixed liquid at a temperature of 40 to 60 DEG C to obtain a blocky polymer, then crushing, grinding, sieving, repeatedly carrying out sedimentation by acetone, drying to obtain polymer particles, repeatedly carrying out Soxhlet extraction on the polymer particles and drying to obtain the epothilone molecularly imprinted polymer. The epothilone molecularly imprinted polymer prepared by the method has higher affinity on epothilone, has a better adsorption effect and has wide application value and market prospect.

Description

technical field [0001] The invention belongs to the technical field of molecular imprinting, and in particular relates to a method for preparing epothilone molecular imprinting polymers by using mixed templates. Background technique [0002] Epothilone (epothilone) is a class of macrolide compounds produced by the myxobacterium Sorangium cellulosum. It has a variety of biological activities and can be used as an antibiotic drug. Its components can be divided into Epothilone A, B, C, D, etc. Studies have shown that it not only has the same activity of stabilizing microtubules as Zibinol, but also has better water solubility than Zibinol, which is a research hotspot of anti-tumor drugs at present. [0003] At present, there are still many defects in the production of epothilones: on the one hand, in the fermentation process, the toxicity and feedback inhibition of epothilones to the production strains make it difficult to increase the fermentation yield of epothilones. Altho...

AI Technical Summary

Problems solved by technology

[0003] At present, there are still many defects in the production of epothilones: on the one hand, in the fermentation process, the toxicity and feedback inhibition of epothilones to the production strains make it difficult to increase the fermentation yield of epothilones. Although the difficulties have been alleviated to some extent by adding the macroporous adsorption resin XAD-16 in the fermentation broth, the non-specific adsorption of the nutrients in the fermentation broth by the resin has brought many new difficulties to the increase of the yield of epothilone; On the other hand, the separation and extraction process steps are very cumbersome, the separation efficiency is low, more organic solvents are used, the selectivity is low and the cost is high

However, domestic and foreign scholars have published few articles on epothilone molecularly imprinted polymers, and most of them use a single template molecule, so the synthesized polymers do not have high affinity.