Application of Pseudomonas aeruginosa strain fermentation broth extract and validamycin compound

A technology of Pseudomonas aeruginosa and Jinggangmycin, which is applied in the application, chemicals for biological control, fungicides, etc., can solve the problems of less research on phytopathogenic bacteria, and achieve the expansion of the antibacterial spectrum, the simple fermentation process, The effect of convenient operation

Inactive Publication Date: 2014-04-23
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because the bacteria can produce a variety of antibacterial substances, the antibacterial substances produced by different strains are different, and the same strain can also produce a variety of antibacterial substances, and these antibacterial substances have inhibitory effects on plant pathogenic fungi, but their extracts and There are few studies on the inhibitory effect of Jinggangmycin compound on plant pathogenic fungi, and less research on plant pathogenic bacteria. Only its metabolite pyocyanin has inhibitory effect on bacteria, and other metabolites have research on plant pathogenic bacteria There is no report

Method used

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  • Application of Pseudomonas aeruginosa strain fermentation broth extract and validamycin compound
  • Application of Pseudomonas aeruginosa strain fermentation broth extract and validamycin compound
  • Application of Pseudomonas aeruginosa strain fermentation broth extract and validamycin compound

Examples

Experimental program
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Effect test

Embodiment 1

[0030] The preparation of embodiment 1 bacterial strain of the present invention

[0031] Antagonistic bacteria were screened from rice-duck co-culture fields in Liuyang City by dilution separation method. Accurately weigh 10g of duck manure and put it into 90mL of sterile water with glass beads, shake it for 30min, take the suspension after standing for 10min, and dilute to 1×10 -7 times, and then the diluted solution was spread on the beef extract peptone agar medium plate and cultivated to obtain a single colony. The obtained single colony is made into a suspension with sterile water and made into a filter paper sheet, which is stored on a potato agar medium plate at a distance of 2 cm from both sides of the central rice sheath blight bacteria cake (5mm) and cultivated on a potato agar medium plate, indicating that the bacterial colony After the plate is covered, the bacterial colony with the inhibition zone is picked and purified repeatedly to obtain a single colony, whic...

example 2

[0032] Activation and fermentation of example 2 bacterial strains of the present invention

[0033] Using an inoculation loop, the bacterial strain SU8 of the present invention is inserted into the beef extract peptone slant medium (beef extract 3-6g, peptone 8-10g, sodium chloride 4-5g, glucose 15-20g, water 1000mL, pH is 7.2-7.3), Incubate at 28°C for 24h. Then, 10% of the inoculum was added to the beef extract peptone liquid medium, the magnetic stirrer rotated at 1000r / min, and placed in a 28-30°C shaking incubator for 84 hours of continuous culture to obtain a fermentation broth.

example 3

[0034] The extraction of example 3 antibacterial substances

[0035] Take the fermented broth and ethyl acetate at a ratio of 1:2, mix well, soak and extract for 48 hours, put the extract into a rotary evaporator at a temperature of 40°C, and rotate at a speed of 110r / min until it becomes a paste to obtain antibacterial substances.

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Abstract

The invention belongs to the technical field of biopesticides, and in particular relates to an antagonistic bacteria-Pseudomonas aeruginosa SU8. The Pseudomonas aeruginosa SU8 is preserved in China Center for Type Culture Collection in May 7th, 2013, the preservation number is CCTCC NO:M2013178. Ethyl acetate extract of Pseudomonas aeruginosa SU8 fermentation broth is compounded with validamycin to achieve the synergism on preventing and controlling rice sheath blight disease, and the ethyl acetate extract has an inhibiting effect on Xanthomonas oryzae pv. Oryzicola and Xanthomonas axonopodis pv. Citri.

Description

[0001] This application is a divisional application with the application number 201310250195.0, the application date is June 21, 2013, and the invention name is a strain of Pseudomonas aeruginosa. technical field [0002] The invention belongs to the technical field of biopesticides, in particular to antagonistic bacteria-Pseudomonas aeruginosa (Pseudomonas aeruginosa) SU8, and also relates to the application of the strain. Background technique [0003] In agricultural production, chemical pesticides are often used to prevent and control crop diseases. Although chemical pesticides have an ideal control effect on crop diseases, the negative problems brought about by chemical pesticides have become increasingly prominent, such as: polluting the environment, destroying natural enemies, and long-term use can easily induce pathogenic bacteria to develop drug resistance. Secondly, the excessive application of chemical pesticides not only increases the cost of medication, but also ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P3/00A01N43/16
Inventor 廖晓兰张亚苏品黄璜刘双清
Owner HUNAN AGRICULTURAL UNIV
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