Holothurian culture water body remediation agent prepared by mixed fermentation process and preparation method and application thereof

A technology for mixed-bacteria fermentation and aquaculture water, which is applied in the field of aquaculture and marine microorganisms. It can solve the problems of excessive chemical residues and antibiotic residues, pollution of aquaculture water, high mortality of sea cucumbers, etc., and achieves good suspension and controllability of sinking and floating. Avoid acidification and odor, reduce the effect of stress response

Inactive Publication Date: 2014-04-23
DALIAN SEM BIOLOGICAL ENG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the current situation in the prior art, such as serious pollution of breeding water quality, frequent occurrence of diseases, high mortality rate of sea cucumbers, excessive heavy metals, chemical residues and antibiotic residues in sea cucumbers, etc., the present invention provides sea cucumber breeding water repair agent and its preparation by mixed bacteria fermentation method. law and use

Method used

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  • Holothurian culture water body remediation agent prepared by mixed fermentation process and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0037] 1. Preparation of probiotic stock solution

[0038] Add Bacillus subtilis YB-1 CGMCC No.5116, Bacillus cereus YB-2CGMCC No.5117 and Bacillus flexus XH-1 CGMCC No.5115 that grew well on LB agar slant medium to 2116E medium respectively, pH7.0~7.5, shake culture at 37℃ for 12~16h, then mix with volume ratio of 1:1:1 to prepare probiotic stock solution, the concentration of bacteria is about 1×10 8 cfu / ml.

[0039] 2. Liquid fermentation culture

[0040] Add fermentation broth to the closed fermentation reactor, and add nutrients at a volume ratio of 0.1 to 1%, then inoculate the probiotic stock solution prepared in step ① at a volume ratio of 3-5%, and carry out fermentation at a pressure of 0.1~0.25Mpa, ferment at 37°C for 24 hours to obtain the fermentation product;

[0041] The above nutrients are composed of corn flour: soybean meal flour: seawater in a weight ratio of (1.5-3): (0.5-1.5): 100.

[0042] 3. Spray drying treatment

[0043] The composite adsorption c...

Embodiment 2

[0046] 1. Preparation of probiotic stock solution

[0047] Add Bacillus subtilis YB-1 CGMCC No.5116, Bacillus cereus YB-2CGMCC No.5117 and Bacillus flexus XH-1 CGMCC No.5115 that grew well on LB agar slant medium to 2116E medium respectively, pH 7.0-7.5, shake culture at 37°C for 12-16 hours, and then mix at a volume ratio of 1:1:1 to prepare a probiotic stock solution with a cell concentration of about 1×108cfu / ml.

[0048] 2. Liquid fermentation culture

[0049] Add fermentation broth to the closed fermentation reactor, and add nutrients at a volume ratio of 0.1 to 1%, then inoculate the probiotic stock solution prepared in step ① at a volume ratio of 3-5%, and carry out fermentation at a pressure of 0.1~0.25Mpa, ferment at 37°C for 24 hours to obtain the fermentation product;

[0050] The above nutrients are composed of corn flour: soybean meal flour: seawater in a weight ratio of (1.5-3): (0.5-1.5): 100.

[0051] 3. Spray drying treatment

[0052] The adsorption carrie...

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Abstract

The invention relates to a holothurian culture water body remediation agent prepared by a mixed fermentation process and a preparation method and application thereof. Based on physiological habits and culture characteristics of holothurian the holothurian culture water body remediation agent consists of bacillus flexus CGMCC No.5115, bacillus subtilis CGMCC No.5116 and bacillus cereus CGMCC No.5117 which are separated and screened in a holothurian culture environment according to a marine microorganism technology. The preparation method comprises the steps: fermenting by using corn flour and bean pulp as main culture raw materials at a temperature of 28-37 DEG C for 24-48h, and processing by using zeolite, kieselguhr and rice hull as adsorption and suspension compound carriers, to obtain the holothurian culture water body remediation agent prepared by the mixed fermentation process. The holothurian culture water body remediation agent has high water environment tolerance and good floatability, suspension property and floating controllability, is beneficial to a three-dimensional purification effect of a seawater culture water body, and has the advantages of preventing the water body from being acidized or suffering from odor bottom, effectively inhibiting pathogen microorganisms, preventing holothurian diseases, and improving yield and quality of holothurian culture.

Description

technical field [0001] The invention belongs to the technical field of aquaculture and marine microbes, and in particular relates to a sea cucumber cultivation water restoration agent prepared by a mixed-bacteria fermentation method, its preparation method and application. Background technique [0002] Seawater is the basic environmental condition for the survival of sea cucumbers and other sea treasures. As the saying goes: "Three points for fish, seven points for water". The quality of water quality directly affects the growth status of sea cucumbers and the economic benefits of farmers. With the development of economy and science, people's demand for sea cucumbers is increasing day by day. Under the circumstances that natural fishing cannot meet the market demand, the sea cucumber farming industry has transformed from the traditional extensive management and resource-dependent farming methods to the current intensive and factory farming methods. With the modernization of...

Claims

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Application Information

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IPC IPC(8): C02F3/34A01K61/00
CPCY02A40/81
Inventor 徐永平尤建嵩李淑英李纪彬周俊龙徐牧马欣高玉平刘士茹
Owner DALIAN SEM BIOLOGICAL ENG TECH
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