Preparation method and application of microbial seed coating

A technology of microorganisms and seeds, applied in the field of agricultural biology, can solve the problems of unstable storage of spores, short shelf life, difficulty in large-scale production, etc. Effect

Inactive Publication Date: 2019-06-18
微生物肥料技术研究推广中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, currently the number of Trichoderma-based seed coatings on the market is relatively small, and the active ingredients of Trichoderma coatings are all conidia, such as Trichoderma harzianum from Bywer Corporation in the United States, and Dr. Niu from Shandong Tainuo Pharmaceutical Co., Ltd.
These preparations all have unstable spore storage and short shelf life. The storage time of live bacteria at room temperature will not exceed 3 months. At the same time, because the conidia are obtained by solid-state fermentation, it is difficult to produce them on a large scale, so the Trichoderma preparations currently on the market cannot completely replace chemical pesticides in plant disease control.
At present, domestic and foreign research on Trichoderma chlamydospore mainly focuses on its production conditions and shake flask culture optimization, and there are few studies on the industrial liquid fermentation and preparation of Trichoderma chlamydospore

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The present invention provides a method for preparing microbial seed coating, including the following steps:

[0031] (1) To provide a fermentation broth of chlamydospores of Trichoderma; the Trichoderma includes Trichoderma harzianum and Trichoderma viride;

[0032] (2) Mix the fermentation broth containing Trichoderma chlamydomen spores with dispersant, film-forming agent and 5% protective agent, and stir for 10-20 minutes at 600r / min;

[0033] (3) After the step (2) is completed, add the binder and the green vegetable seeds, the mass ratio of the binder to the green vegetable seeds is 50:9, and stir for 5 minutes at 600r / min;

[0034] (4) Add cold water with 50% of the remaining protective agent mass to the remaining protective agent, stir evenly, heat to 60°C for 2 minutes until a homogeneous liquid is formed, and cool for later use;

[0035] (5) Mix the fermentation broth obtained in step (3) and the protective agent in step (4) uniformly, and spray-dry the mixed solution to...

Embodiment 2

[0049] Take the seed coated with microbial seeds prepared in Example 1 as an experimental example, and take 50% boscalid water dispersible granules produced by BASF and clear water as two control examples.

[0050] The experiment was carried out in a greenhouse. The experimental example and the control example were divided into three groups, each with 20 green vegetables, with 3 replicates, and unified simulation of the green vegetables field management environment.

[0051] The results showed that the probability of gray mold in the green vegetables coated with microbial seeds was 5%, and the diseased seedlings would not infect non-diseased seedlings; the probability of gray mold in the green vegetables applied with 50% boscalid water dispersible granules Of the 60 green vegetables in the three groups, 3 strains were found to be infected by diseased seedlings to obtain gray mold; the probability of using clean water to acquire gray mold was 45%, of which 5 plants were infected by d...

Embodiment 3

[0054] The green vegetable seeds coated with microbial seeds prepared in Example 1 were used as the experimental group, and the green vegetable seeds without coating were used as the control group, and the growth experiment was carried out in a laboratory environment without the influence of gray mold disease.

[0055] Experiments show that under the same growth management, the growth of the experimental group with microbial seed coating is better than the control group at all stages, and the greens of the experimental group germinate fast, grow fast, and produce high yield.

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PUM

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Abstract

The invention discloses a preparation method and application of microbial seed coating and belongs to the field of agricultural biotechnology. The preparation method includes the steps of firstly, providing the fermentation liquor of Trichoderma chlamydospores, wherein the Trichoderma comprises Trichoderma harzianum and Trichoderma viride; secondly, mixing the fermentation liquor containing the Trichoderma chlamydospores with a dispersing agent, a film-forming agent and 5% of protecting agent; thirdly, adding an adhesive and seeds after the stirring in the second step is completed; fourthly, adding cold water with the mass being 50% of the mass of the rest of protecting agent into the rest of protecting agent, evenly stirring, heating to 60 DEG C, keeping the temperature for 2 minutes until uniform liquid is formed, and cooling for standby; fifthly, evenly mixing the fermentation liquor obtained in the third step and the protecting agent obtained in the fourth step, and subjecting themixed liquid to spray drying to obtain the microbial seed coating. The preparation method has the advantages that the problems that Trichoderma spores are short in storage time and cannot achieve large-scale production can be solved favorably by using the chlamydospores as the preparation effective component, and the requirements for being used as biological pesticides can be satisfied.

Description

Technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a preparation method and application of microbial seed coating. Background technique [0002] Seed coating agent refers to an active suspending agent that is processed by related medicaments, nutrient elements, film-forming agents, dispersants and auxiliary agents to form a certain strength and permeability that can be directly coated on the surface of the seed. [0003] Seed coating agent is a special preparation that integrates health care, nutrition, protection, and stress resistance. It has the advantages of seed disinfection, prevention and control of pests and rodents, slow release of fertilizer, avoiding damage to natural enemies, reducing environmental pollution, and improving crop resistance. Reverse effects. [0004] At present, in related technologies, the components of common seed coatings are mainly active components and inactive components; the active components in...

Claims

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Application Information

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IPC IPC(8): A01N25/28A01N63/04A01C1/06A01P3/00A01P1/00A01P7/04A01P21/00
Inventor 孟庆伟张静江洋胡志刚吴元凤
Owner 微生物肥料技术研究推广中心
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