Streptomyces phytophytes and method and application thereof for producing antibiotic novonidocycin
A technology for planting Streptomyces sp. and nidomycin, which can be applied in microorganism-based methods, botanical equipment and methods, biochemical equipment and methods, etc., and can solve the problems of difficulty in discovering new antibiotics and limited natural resources.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
example 1
[0080] Example 1: The soil sample numbered S1179 was collected from Wawushan, Sichuan, and an actinomycete Streptomyces phytohabitans was isolated by actinomycete dilution separation method, and the strain code was HBERC-20821.
[0081] Separation steps:
[0082] (1) Weigh 1 gram of soil sample, put it into a 150ml Erlenmeyer flask with glass beads, add sterile water (containing 1% yeast extract), place on a shaker and vibrate at 180 rpm for 1 hour.
[0083] (2) Take 1ml of the above soil sample solution, add 9ml of sterile water, and shake evenly. This is a 10-fold dilution, recorded as 10 -1 .
[0084] (3) Carry out 10-fold serial dilution of the soil sample by the method of (2), until 10 -4 .
[0085] (4) Take serial dilution samples, 10 -2 、10 -3 、10 -4 Each 0.1ml was dropped into different separation medium plates and spread evenly. The medium is ISP-2 (4 grams of yeast extract powder, 10 grams of malt extract powder, 4 grams of glucose) and other formulas.
[00...
example 2
[0097] Example 2: Same as Example 1, difference
[0098] The fermentation of two antibiotics produced by Streptomyces phytophytes uses a 20-liter fermenter,
[0099] (1) Production of glycerol cryopreservation tubes: formula in seed medium.
[0100]Seed medium formula: 2% mannitol, 2% soybean peptone, 0.4% yeast extract powder, 0.4% calcium carbonate, and 0.5% sodium chloride. pH 6.5-7.5, 100mL per bottle;
[0101] (2) Shake flask seed preparation: Due to the large fermentation volume, the seeds need to be enlarged to meet the inoculum volume requirement. Inoculate the shake flask seeds at 10% (V / V). The culture conditions and methods are the same as the shake flask seeds, but the culture time is 1 to 2 days. Sampling and microscopic examination will obtain qualified strains;
[0102] (3) fermentation: after the seed bottle grows well, transfer in the fermenter by the inoculum size of 5% (V / V), and the filling capacity of culture medium is 12 liters. The stirring speed of ...
example 3
[0108] Example 3: Same as Example 2, the difference is that
[0109] Fermentation of two antibiotics produced by Streptomyces phytophytes in a 50-liter fermenter
[0110] (1) The formula of the fermentation medium is: 3% mannitol, 3% soybean peptone, 0.5% yeast extract, 0.5% calcium carbonate, and 0.8% sodium chloride. pH value 6.5~7.5;
[0111] (2) The rotating speed of the shaking table is 160 rpm, and the rotating speed of the fermenter is 200 rpm;
[0112] (3) The capacity of the fermentation tank is 30 liters;
[0113] (4) After the fermentation is finished, centrifuge with a high-speed tube centrifuge to obtain supernatant and mycelium. Finally, get 400 grams of novomycin crude extract, which contains two kinds of antibiotics.
PUM
| Property | Measurement | Unit |
|---|---|---|
| diameter | aaaaa | aaaaa |
| particle diameter | aaaaa | aaaaa |
| diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More