Production method of fermented black garlics
A production method and technology for black garlic, applied in food preparation, food science, application and other directions, can solve the problems of no specific quantification of black garlic change rules, not too many changes, long fermentation time, etc., to enhance human immunity, Restoring human fatigue, high curative effect
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Embodiment 1
[0022] Embodiment 1: single factor analysis of fermentation condition
[0023] According to the fermentation principle of black garlic, the single factor analysis of total polyphenols production in five stages is as follows:
[0024] (1) Preparation stage: The garlic used is 14~16 cloves of garlic. After the commercially available garlic is removed from soil and impurities, it is used to make fermented black garlic; the instrument used is a constant temperature and humidity incubator (the temperature control range is 10~90 ℃; the humidity control range is 10%~95%; the time control range is 0~999h).
[0025] (2) Fermentation stage
[0026] Treatment 1: Select the temperature of 70, 80 and 90°C in the first stage, the humidity of 70%, 80% and 90% respectively, and the fermentation time of 70, 80 and 90h; the temperature of 50°C and the humidity of 80% in the second stage , time 50h; third stage choose temperature 85°C, humidity 80%, time 20h; fourth stage: choose temperature 5...
Embodiment 2
[0031] Embodiment 2: Optimizing the production method of fermented black garlic
[0032] (1) Response surface analysis method optimization test design: on the basis of single factor, further optimize the effect of fermentation conditions on the production of various nutrients and antioxidant components in the production process of black garlic, and select the most influential factor, that is, the first The temperature of the first stage, the time of the second stage and the temperature of the fourth stage, the total polyphenols (Y 总多酚 ), reducing sugar (Y 还原糖 ) and FRAP (Y FRAP) generation is the response value, and the level arrangement and results of each factor are shown in Table 1. Other conditions: the first stage humidity is 90%, 70h; the second stage selects temperature 50°C, humidity 90%; 90%, time 20h; fourth stage: humidity 40%, time 80h; fifth stage choose 25 ℃, place 100h.
[0033] Table 1 Response surface analysis factors and test results
[0034]
[0035] ...
Embodiment 3
[0045] Example 3: Characteristic Evaluation of Fermented Black Garlic
[0046] Utilize the method of embodiment 2 to produce fermented black garlic, compare and evaluate the difference with the nutrient composition and antioxidant capacity in common fresh garlic; Wherein water, fat, protein, total amino acid, reducing sugar are respectively by GB 5009.3-2010, GB / T 5009.6—2003, GB / T 5009.5—2010, GB / T 5009.124—2003, 3,5-dinitrosalicylic acid colorimetric method; the determination of total polyphenol content adopts Folin-Ciocalteau method, with gallic acid ( Gallic acid) as a standard curve, the total polyphenol content of the sample is expressed in g per kg of gallic acid (Galice acid) required to achieve the same absorbance (g GAE / kg); alliin and S-allyl-L half The content of cystine sulfoxide was determined by high-performance liquid chromatography, using Shimadzu LC-6A (Shimadzu, Japan) high-performance liquid chromatography, and the chromatographic column was RP C18 column ...
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