Method for preparing organic fertilizer
An organic fertilizer and seed technology, which is applied in the preparation of organic fertilizers, organic fertilizers, and the treatment of biological organic parts, can solve the problems of unrealized resource utilization, extensive production, and difficulty in quality assurance, and achieve value-added and resource utilization , short production cycle and stable process
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Embodiment 1
[0038] A preparation method for producing organic fertilizer using alcohol anaerobic distiller's grains as main raw material, comprising the following steps:
[0039] (1) Preparation of compound fermentation culture medium
[0040] a. Preparation of culture medium
[0041] The first seed activated solid medium: glucose 20g / L, yeast extract 5g / L, peptone 10g / L, agar 15g / L, distilled water to 1000mL, pH value natural, sterilized with 0.1MPa high-pressure steam for 30 minutes Beveled back swing.
[0042] The second seed activated solid medium: ammonium chloride 8g / L; disodium hydrogen phosphate 0.7g / L; magnesium sulfate 1.8g / L; sodium chloride 2g / L; sodium bicarbonate: 50g / L; yeast extract: 20g / L ethanol 1.8ml / L, agar 18g / L, adjust the pH to 7.0 with 0.1mol / L phosphoric acid, adjust the volume to 1000mL with distilled water, sterilize with 0.1MPa high-pressure steam for 30 minutes, and set the inclined plane.
[0043] The third seed activated solid medium: glucose 8g / L, yeast...
Embodiment 2
[0063] A preparation method for producing organic fertilizer using alcohol anaerobic distiller's grains as main raw material, comprising the following steps:
[0064] (1) Preparation of compound fermentation culture medium
[0065] a. Preparation of culture medium
[0066] The first seed activated solid medium: glucose 15g / L, yeast extract 4.5g / L, peptone 9g / L, agar 15g / L, distilled water to 1000mL, pH value natural, sterilized with 0.1MPa high-pressure steam for 20 - After 30 minutes swing the incline.
[0067] The second seed activated solid medium: ammonium chloride 10g / L; disodium hydrogen phosphate 0.5g / L; magnesium sulfate 2g / L; sodium chloride 1.8g / L; sodium bicarbonate: 45g / L; yeast extract: 15g / L ethanol 2ml / L, agar 20g / L, adjust the pH to 7.0 with 0.1mol / L phosphoric acid, adjust the volume to 1000mL with distilled water, sterilize with 0.1MPa high-pressure steam for 25 minutes, and set the inclined plane.
[0068] The third seed activated solid medium: glucose 1...
Embodiment 3
[0088] A preparation method for producing organic fertilizer using alcohol anaerobic distiller's grains as main raw material, comprising the following steps:
[0089] (1) Preparation of compound fermentation culture medium
[0090] a. Preparation of culture medium
[0091] The first seed activated solid medium: glucose 20g / L, yeast extract 4g / L, peptone 8g / L, agar 20g / L, distilled water to 1000mL, pH value natural, sterilized with 0.1MPa high-pressure steam for 20 minutes ) back swing slope.
[0092] Second seed activation solid medium: ammonium chloride 8g / L; Disodium hydrogen phosphate 0.5g / L; Magnesium sulfate 1.7g / L; Sodium chloride 1.5g / L; Sodium bicarbonate: 48g / L; 20g / L ethanol 1.4ml / L, agar 15g / L, adjusted to pH 7.0 with 0.1mol / L phosphoric acid, adjusted to 1000mL with distilled water, sterilized with 0.1MPa high-pressure steam for 30 minutes) and placed the inclined plane.
[0093] The third seed activated solid medium: glucose 12g / L, yeast extract 10g / L, calcium ca...
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