Method for producing malic acid by using fermentation of wheat bran hydrolysate

A technology of wheat bran and wheat bran powder, which is applied in the field of fermentation, can solve the problems of low utilization rate of wheat bran, and achieve the effects of alleviating energy crisis, high conversion rate and low sugar loss

Inactive Publication Date: 2014-05-14
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for producing malic acid b

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0021] Example 1

[0022] A. Preparation of wheat bran hydrolysate

[0023] The wheat bran is impurity removed, placed in a hot air drying oven, and dried at 80°C for several hours. The dried wheat bran is crushed by a plant crusher, passed through a 60-mesh sieve, and the wheat bran powder is placed in a sealed bag for later use.

[0024] Take 200g of wheat bran powder, add 2.0L of dilute sulfuric acid solution with a mass concentration of 3%, stir and mix, react at 121℃ for 1h, cool, filter, take the filtrate, add excess Ca(OH) to the filtrate 2 Adjust the pH to 10-11, centrifuge at 8000r / min for 10min, take the supernatant, add 3% dilute sulfuric acid to the supernatant to adjust the pH to neutral, centrifuge at 8000r / min for 10min, take The supernatant is the hydrolyzed sugar of wheat bran.

[0025] B. Detoxification treatment of hydrolysate

[0026] 200g activated carbon was added to the wheat bran hydrolysate obtained in step A, and placed in a water bath shaker at 40°C and 120r...

Example Embodiment

[0035] Example 2

[0036] A. Preparation of wheat bran hydrolysate

[0037] The wheat bran is impurity removed, placed in a hot air drying oven, and dried at 80°C for several hours. The dried wheat bran is crushed by a plant crusher, passed through a 60-mesh sieve, and the wheat bran powder is placed in a sealed bag for later use.

[0038] Take 15kg of wheat bran powder, add 150L of dilute sulfuric acid solution with a mass concentration of 3%, stir and mix, react at 121℃ for 1h, cool, filter, take the filtrate, add excess Ca(OH) to the filtrate 2 Adjust the pH to 10-11, centrifuge at 8000r / min for 10min, take the supernatant, add 3% dilute sulfuric acid to the supernatant to adjust the pH to neutral, centrifuge at 8000r / min for 10min, take The supernatant is the hydrolyzed sugar of wheat bran.

[0039] B. Detoxification treatment of hydrolysate

[0040] 15kg of activated carbon was added to the wheat bran hydrolysate obtained in step A, and placed in a water bath shaker at 40°C and 1...

Example Embodiment

[0049] Example 3

[0050] A. Preparation of wheat bran hydrolysate

[0051] The wheat bran is impurity removed, placed in a hot air drying oven, and dried at 80°C for several hours. The dried wheat bran is crushed by a plant crusher, passed through a 60-mesh sieve, and the wheat bran powder is placed in a sealed bag for later use.

[0052] Take 100g of wheat bran powder, add 1.0L of dilute sulfuric acid solution with a mass concentration of 3%, stir and mix, react at 121℃ for 1h, cool, filter, take the filtrate, add excess Ca(OH) to the filtrate 2 Adjust the pH to 10-11, centrifuge at 8000r / min for 10min, take the supernatant, add 3% dilute sulfuric acid to the supernatant to adjust the pH to neutral, centrifuge at 8000r / min for 10min, take The supernatant is the hydrolyzed sugar of wheat bran.

[0053] B. Detoxification treatment of hydrolysate

[0054] 100g of activated carbon was added to the wheat bran hydrolysate obtained in step A, and placed in a water bath shaker at 40°C and 1...

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Abstract

The invention provides a method for producing malic acid by using fermentation of wheat bran hydrolysate. The method comprises the following steps: preparing wheat bran hydrolysate by an acid hydrolysis method, and then carrying out detoxification treatment on a fermentation inhibitor contained in the hydrolysis liquid glucose by using activated carbon; and selecting aspergillus parasiticus CICC40365 with good adaptability, and matching with the optimized fermentation nutrients to mix and ferment, so as to obtain malic acid fermentation liquor. The method has advantages of being high in wheat bran hydrolysis rate, fast in reaction speed, simple in process and low in cost, the hydrolysis liquid glucose is low in loss in the fermentation process by good detoxification treatment, and the adopted aspergillus has good adaptability, and is high in conversion rate of the malic acid. In addition, the method is free of poison and harm in the production process, environment-friendly and energy-saving, applicable to industrial large-scale production, and significant in economic benefits may be achieved, energy crisis in China and in the world can be effectively relieved.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a method for producing malic acid by fermenting wheat bran hydrolysis sugar. Background technique [0002] L-malic acid (L-malic acid), also known as hydroxysuccinic acid, is a saturated dibasic acid. Appearance is white crystal or crystalline powder, has strong hygroscopicity, easily soluble in water and ethanol, has a special sour taste, a natural organic acid widely present in vegetables and fruits, its content is second only to citric acid, and Has a special flavor. In addition, L-malic acid is also an important intermediate product in human metabolism, which is easily absorbed by the human body. Therefore, it is widely used in food, cosmetics, medicine and other fields as an excellent sour agent and fresh-keeping agent. [0003] Wheat is one of the main food crops in my country, and its annual output has exceeded 100 million tons. Wheat bran is the main ...

Claims

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Application Information

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IPC IPC(8): C12P7/46C12R1/66
Inventor 李兴江吴悦潘丽军姜绍通李硕刘亚孙婷
Owner HEFEI UNIV OF TECH
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