Artery blood vessel simulation microfluid control device enabling direct observation under high-power objective

Abstract

The invention discloses an artery blood vessel simulation microfluid control device enabling direct observation under a high-power objective. The device is provided with a transparent microfluid channel module (1), an elastic membrane (2), a negative pressure generation module (3) and a cover glass (10) sequentially from top to bottom, wherein a microfluid channel (8) is arranged at the bottom of the microfluid channel module (1) and used for enabling fluid flow; the negative pressure generation module (3) is internally provided with a negative pressure hollow groove (9) going through the top to bottom and used for generating negative pressure enabling the elastic membrane to deform; the total thickness of the elastic membrane (2) and the cover glass (10) is less than or equal to 300 microns. The microfluid control device disclosed by the invention not only can simulate the physiological and pathological conditions of the organs and tissues bearing the fluid shear stress and mechanical drawing force in vivo at the same time, but also enables direct observation under the high-power objective so as to realize real-time observation of the dynamic and static change in the fine structure and microfluid channel of a single cell; therefore, a more effective tool is provided to the related basic research and the drug screening for artery blood vessel diseases.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to an arterial blood vessel simulation microfluidic device that can be directly observed under a high-magnification objective lens. Background technique [0002] Abnormal hemodynamic factors are one of the key risk factors leading to cardiovascular and cerebrovascular diseases, but its mechanism of action is still unclear, and the limitations of traditional research methods hinder the progress of related research. In recent years, the establishment and application of vascular research models in vitro have greatly promoted the progress of related research. [0003] The hemodynamic research models of blood vessels in vitro can be divided into three categories according to the types of mechanical stimuli that they simulate when blood flows through blood vessels, namely fluid shear force model, stretch stress model, and the combined action of fluid shear force and stretch stress Mo...

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Problems solved by technology

However, the above-mentioned microfluidic devices are only suitable for alveolar models

[0011] Zheng et al (Zheng W, Jiang B, Wang D, Zhang W, Wang Z, Jiang X. (2012) A microfluidic flow-stretch chip for investigating blood vessel biomechanics.Lab Chip.Sep21;12(18):3441-50 ) The device used can be used for the study of applying fluid shear force alone, mechanical stretching force alone or simultaneous application of fluid shear force and mechanical stretching force to cells, but it cannot observe the dynamic changes of cells under a high-power microscope

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