Artery blood vessel simulation microfluid control device enabling direct observation under high-power objective

An arterial vessel simulation and microfluidic device technology, applied in the field of biomedicine, can solve the problem of inability to observe the dynamic changes of cells, and achieve the effects of being easy to manufacture and use, promoting progress, and having a simple structure

Active Publication Date: 2014-05-21
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above-mentioned microfluidic devices are only suitable for alveolar models
[0011] Zheng et al (Zheng W, Jiang B, Wang D, Zhang W, Wang Z, Jiang X. (2012) A microfluidic flow-stretch chip for investigating blood vessel biomechanics.Lab Chip.Sep21;12(18):3441-50 ) The

Method used

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  • Artery blood vessel simulation microfluid control device enabling direct observation under high-power objective
  • Artery blood vessel simulation microfluid control device enabling direct observation under high-power objective
  • Artery blood vessel simulation microfluid control device enabling direct observation under high-power objective

Examples

Experimental program
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Effect test

Embodiment 1

[0051] Example 1 Stretch detection of the elastic membrane during the operation of the arterial blood vessel simulation microfluidic device of the present invention

[0052] Add HeLa cells to the microfluidic channel in the arterial simulating microfluidic device of the present invention, and make the cells adhere to the elastic membrane under the conditions of 37°C and 5% carbon dioxide; Observe the cells on the elastic membrane, the results are as follows figure 2 As shown, the stretch rate of the elastic film after negative pressure stretching is about 8% compared with that before stretching.

Embodiment 2

[0053] Example 2 Observation of the fine structure of cells in the arterial vessel simulation microfluidic device of the present invention under a high-magnification objective lens

[0054] Human umbilical vein endothelial cells are added to the microfluidic channel of the arterial vessel simulation microfluidic device of the present invention, and the cells are attached to the elastic membrane under the conditions of 37°C and 5% carbon dioxide; the mechanical conditions of the arterial vessel are simulated for cell culture 2 Hours later, the cells were fixed, and the nuclei and microfilaments were stained. Under the stretched condition of the elastic membrane, the fine structure of the cells on the elastic membrane was observed with a 63-fold lens, and the results were as follows image 3 As shown, the fine structure of cells can be clearly observed.

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Abstract

The invention discloses an artery blood vessel simulation microfluid control device enabling direct observation under a high-power objective. The device is provided with a transparent microfluid channel module (1), an elastic membrane (2), a negative pressure generation module (3) and a cover glass (10) sequentially from top to bottom, wherein a microfluid channel (8) is arranged at the bottom of the microfluid channel module (1) and used for enabling fluid flow; the negative pressure generation module (3) is internally provided with a negative pressure hollow groove (9) going through the top to bottom and used for generating negative pressure enabling the elastic membrane to deform; the total thickness of the elastic membrane (2) and the cover glass (10) is less than or equal to 300 microns. The microfluid control device disclosed by the invention not only can simulate the physiological and pathological conditions of the organs and tissues bearing the fluid shear stress and mechanical drawing force in vivo at the same time, but also enables direct observation under the high-power objective so as to realize real-time observation of the dynamic and static change in the fine structure and microfluid channel of a single cell; therefore, a more effective tool is provided to the related basic research and the drug screening for artery blood vessel diseases.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to an arterial blood vessel simulation microfluidic device that can be directly observed under a high-magnification objective lens. Background technique [0002] Abnormal hemodynamic factors are one of the key risk factors leading to cardiovascular and cerebrovascular diseases, but its mechanism of action is still unclear, and the limitations of traditional research methods hinder the progress of related research. In recent years, the establishment and application of vascular research models in vitro have greatly promoted the progress of related research. [0003] The hemodynamic research models of blood vessels in vitro can be divided into three categories according to the types of mechanical stimuli that they simulate when blood flows through blood vessels, namely fluid shear force model, stretch stress model, and the combined action of fluid shear force and stretch stress Mo...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/00G01N33/68
CPCC12M21/08C12M23/16C12M25/02C12M35/04
Inventor 蒋兴宇黄蓉郑文富张伟
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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