Method for detecting clenbuterol hydrochloride based on fluorescence resonance energy transfer

A clenbuterol hydrochloride, fluorescence resonance energy technology, applied in the field of analytical chemistry, can solve the problems of high cost, long measurement time, not suitable for rapid detection, etc., to achieve the effect of rapid detection and convenient supervision

Inactive Publication Date: 2014-05-21
JILIN UNIV
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Problems solved by technology

Although these methods have high sensitivity, these methods require expensive equipment, long measurement time, high cost, professional and technical personnel are required to operate, and are not suitable for on-site rapid detection

Method used

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  • Method for detecting clenbuterol hydrochloride based on fluorescence resonance energy transfer
  • Method for detecting clenbuterol hydrochloride based on fluorescence resonance energy transfer
  • Method for detecting clenbuterol hydrochloride based on fluorescence resonance energy transfer

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Embodiment Construction

[0035] Preparation of gold nanoparticles AuNPs

[0036] Materials / Reagents: Chlorauric acid and trisodium citrate were purchased from Beijing Chemical Plant.

[0037]Method: During preparation, add 100mL of 1mmol / L chloroauric acid into a clean three-necked flask equipped with a reflux device, boil it under magnetic stirring, and then quickly add 10mL of 38.8mmol / L trisodium citrate , stirring while heating, until the solution turns from light yellow to wine red, the reaction lasts for 10 minutes, stop heating, cool the solution to room temperature, and store at 4°C.

[0038] Results: Characterized by transmission electron microscopy, the particle size of the prepared gold nanoparticles was 13nm, with good dispersion and uniform particle size.

[0039] Since the system used in the experiment is affected by the concentration of gold nanoparticles, it is necessary to study the influence of the concentration of gold nanoparticles on the reaction system.

[0040] Materials / reage...

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Abstract

The invention discloses a method for detecting clenbuterol hydrochloride based on fluorescence resonance energy transfer, and belongs to the technical field of analytic chemistry. The method is characterized by detecting residual quantity of clenbuterol hydrochloride by using a method of fluorescence quenching effect of gold nano particles to Rhodamine B; the detection steps include preparation of the gold nano particles (AuNPs), observation of the reaction of the Rhodamine B, the gold nano particles and a clenbuterol hydrochloride system by measuring a fluorescence spectrogram and an ultraviolet absorption spectrogram, detection on the clenbuterol hydrochloride by using the method of fluorescence quenching effect of gold nano particle to Rhodamine B, and detection on the actual sample. The method is capable of simply, fast and sensitively detecting the clenbuterol hydrochloride, is high in sensitivity, and provides convenience for the following research, production and supervision.

Description

technical field [0001] The invention discloses a method for detecting clenbuterol hydrochloride based on fluorescence resonance energy transfer, which belongs to the technical field of analytical chemistry. Background technique [0002] Clenbuterol hydrochloride, also known as "clenbuterol", is a poisonous accumulation anti-asthma drug and an adrenal nerve stimulant. A company in the United States accidentally discovered that clenbuterol hydrochloride can significantly promote animal growth and increase lean meat percentage. This new discovery was soon used by some countries in the breeding industry. Adding clenbuterol hydrochloride to the feed can increase the growth rate, feed conversion rate, and carcass lean meat rate of pigs and other livestock and poultry. Since the price of lean meat is higher than that of fat meat, farmers are willing to use clenbuterol to raise pigs to increase the lean meat rate. [0003] However, if the pork containing "clenbuterol hydrochloride...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 孙春燕许景月李莹郭佳佳曹先一罗叶丽沈斐
Owner JILIN UNIV
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