Method for extracting Humulus scandens (Lour.) Merr. leaf protein and total flavonoids in graded manner simultaneously from fresh and tender stems and leaves of Humulus scandens (Lour.) Merr.

A technology for fractional extraction and total flavonoids, which is applied in the preparation method of peptides, chemical instruments and methods, medical preparations containing active ingredients, etc., can solve the problems of inability to use Humulus japonicus resources in depth, time-consuming, repeated processing, etc., and achieve extraction The process conditions are accurate and reliable, and the effect of improving the value of development and utilization

Inactive Publication Date: 2014-05-28
SUZHOU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

At present, the methods for extracting Humulus leaf protein and flavonoids are separated, and they are all for the purpose of extracting a single substance, which leads to repeated processing, time-consuming, inability to deeply utilize Humulus resources, and waste of Humulus resources

Method used

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  • Method for extracting Humulus scandens (Lour.) Merr. leaf protein and total flavonoids in graded manner simultaneously from fresh and tender stems and leaves of Humulus scandens (Lour.) Merr.
  • Method for extracting Humulus scandens (Lour.) Merr. leaf protein and total flavonoids in graded manner simultaneously from fresh and tender stems and leaves of Humulus scandens (Lour.) Merr.
  • Method for extracting Humulus scandens (Lour.) Merr. leaf protein and total flavonoids in graded manner simultaneously from fresh and tender stems and leaves of Humulus scandens (Lour.) Merr.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] refer to figure 1 As shown, the method for extracting Humulus leaf protein and total flavonoids simultaneously from the fresh and tender stems and leaves of Humulus comprises the following steps:

[0018] Step 1, take the fresh and tender humulus stems and leaves collected in August, wash them with distilled water, dry the surface water at room temperature, measure their water content and weight, and cut them into about 0.5cm long fragments;

[0019] Step 2, adding the stems and leaves of Humulus japonicus obtained in step 1 to 10 times the weight of water, making it into a homogenized form with a high-speed homogenizer, and then completely filtering out the juice;

[0020] Step 3, on the basis of the single factor experiment, according to the central combination design principle of Box-Behnken, take beating time, leaf protein sedimentation temperature and leaf protein sedimentation pH three factors as independent variables (respectively with X 1 , X 2 , X 3 Indicate...

Embodiment 2

[0088] The method for extracting humulus leaf protein and total flavonoids simultaneously and fractionally from the fresh and tender stems and leaves of Humulus japonicus comprises the following steps:

[0089] Step 1, wash 10Kg of the fresh and tender branches and leaves of Humulus humulus collected in August with distilled water, dry the surface water at normal temperature, then measure its water content, and its water content is 75%. Weigh (wet weight) the stems and leaves of Humulus humulus taken, and cut them straight into about 0.5cm length fragments as a basis for measurement, and set aside for later use.

[0090] Step 2: add the stems and leaves of Humulus japonicus treated in step 1 into distilled water with a weight 10 times higher, beat for 5.5 minutes with a high-speed homogenizer, make it into a homogenized slurry, and then filter out the juice completely.

[0091] In step 3, the humulus juice obtained in step 2 is subjected to alkaline precipitation at 71°C and p...

Embodiment 3

[0101] Step 1, wash 12Kg of fresh and tender branches and leaves of Humulus humulus collected in September with distilled water, dry the surface water at normal temperature, then measure its water content, and its water content is 73%. Weigh (wet weight) the stems and leaves of Humulus humulus taken, and cut them straight into about 0.5cm length fragments as a basis for measurement, and set aside for later use.

[0102] Step 2: add the stems and leaves of Humulus japonicus treated in step 1 into distilled water with a weight 10 times higher, beat for 5.5 minutes with a high-speed homogenizer, make it into a homogenized slurry, and then filter out the juice completely.

[0103] In step 3, the humulus juice obtained in step 2 is subjected to alkaline precipitation at 71°C and pH 10 for 10 minutes, and then centrifuged to obtain supernatant and precipitate, and the precipitate is freeze-dried to obtain Humulus leaf protein. Finally, 813.6 g of humulus leaf protein was obtained, a...

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Abstract

The invention provides a method for extracting Humulus scandens (Lour.) Merr. leaf protein and total flavonoids in a graded manner simultaneously from fresh and tender stems and leaves of Humulus scandens (Lour.) Merr.. The method comprises the steps as follows: firstly, the stems and the leaves of the Humulus scandens (Lour.) Merr. are obtained, the water content and the wet weight are measured after the stems and the leaves are washed and dried by airing, and the stems and the leaves are cut into slices; then distilled water is added for homogenization, press filtration is performed after homogenization to obtain Humulus scandens (Lour.) Merr. juice and filter cakes; the Humulus scandens (Lour.) Merr. juice is subjected to heating, alkaline precipitation and centrifugation to obtain a supernatant liquid and deposits, the deposits are frozen and dried so as to obtain the Humulus scandens (Lour.) Merr. leaf protein; then the filter cakes and the supernatant liquid are mixed, extraction is performed under an alkaline condition, acid precipitation and centrifugation are performed, and after obtained deposits are washed by water, dissolved in alcohol and subjected to suction filtration, a filtrate is concentrated, dried and frozen so as to obtain the Humulus scandens (Lour.) Merr. total flavonoids. According to the method, the Humulus scandens (Lour.) Merr. leaf protein and the Humulus scandens (Lour.) Merr. total flavonoids are extracted in the graded manner simultaneously in one technological process, so that the method is convenient to operate, accurate and reliable in extraction technological condition and high in yield and purity, two important effective components, namely, the leaf protein and the total flavonoids, in the Humulus scandens (Lour.) Merr. can be developed and utilized simultaneously, and development and utilization values of Humulus scandens (Lour.) Merr. resources are increased.

Description

technical field [0001] The invention belongs to a method for extracting plant effective components, in particular to a method for simultaneously extracting humulus leaf protein and total flavonoids in grades from fresh and tender stems and leaves of Humulus japonicus. Background technique [0002] Humulus scandens (Lour.) Merr., also known as Lala rattan, Lala seedling, Pentaclaw, Big-leaved Pentaclaw, Cutting vine, etc., is a herbaceous plant of the genus Humulus in the family Moraceae, distributed in my country. All provinces except Xinjiang and Qinghai are rich in resources. The nutritional components of Humulus chinensis are relatively comprehensive. According to the analysis, the crude fat and crude fiber contents of fresh grass of Humulus chinensis are 0.6% and 2.1% respectively. The dry matter of Humulus japonicus contains 12.8% to 20.46% of crude protein, about 2.03% of total flavonoids, 15.4% to 23.2% of crude fiber, 33.4% to 41.8% of nitrogen-free extract, 7.1% to ...

Claims

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Application Information

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IPC IPC(8): C07K1/14A61K36/60A61K127/00A61K135/00
Inventor 王桃云薛凯陈子欣张慈安闵辉
Owner SUZHOU UNIV OF SCI & TECH
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