Method for extracting Humulus scandens (Lour.) Merr. leaf protein and total flavonoids in graded manner simultaneously from fresh and tender stems and leaves of Humulus scandens (Lour.) Merr.

Abstract

The invention provides a method for extracting Humulus scandens (Lour.) Merr. leaf protein and total flavonoids in a graded manner simultaneously from fresh and tender stems and leaves of Humulus scandens (Lour.) Merr.. The method comprises the steps as follows: firstly, the stems and the leaves of the Humulus scandens (Lour.) Merr. are obtained, the water content and the wet weight are measured after the stems and the leaves are washed and dried by airing, and the stems and the leaves are cut into slices; then distilled water is added for homogenization, press filtration is performed after homogenization to obtain Humulus scandens (Lour.) Merr. juice and filter cakes; the Humulus scandens (Lour.) Merr. juice is subjected to heating, alkaline precipitation and centrifugation to obtain a supernatant liquid and deposits, the deposits are frozen and dried so as to obtain the Humulus scandens (Lour.) Merr. leaf protein; then the filter cakes and the supernatant liquid are mixed, extraction is performed under an alkaline condition, acid precipitation and centrifugation are performed, and after obtained deposits are washed by water, dissolved in alcohol and subjected to suction filtration, a filtrate is concentrated, dried and frozen so as to obtain the Humulus scandens (Lour.) Merr. total flavonoids. According to the method, the Humulus scandens (Lour.) Merr. leaf protein and the Humulus scandens (Lour.) Merr. total flavonoids are extracted in the graded manner simultaneously in one technological process, so that the method is convenient to operate, accurate and reliable in extraction technological condition and high in yield and purity, two important effective components, namely, the leaf protein and the total flavonoids, in the Humulus scandens (Lour.) Merr. can be developed and utilized simultaneously, and development and utilization values of Humulus scandens (Lour.) Merr. resources are increased.

Description

technical field [0001] The invention belongs to a method for extracting plant effective components, in particular to a method for simultaneously extracting humulus leaf protein and total flavonoids in grades from fresh and tender stems and leaves of Humulus japonicus. Background technique [0002] Humulus scandens (Lour.) Merr., also known as Lala rattan, Lala seedling, Pentaclaw, Big-leaved Pentaclaw, Cutting vine, etc., is a herbaceous plant of the genus Humulus in the family Moraceae, distributed in my country. All provinces except Xinjiang and Qinghai are rich in resources. The nutritional components of Humulus chinensis are relatively comprehensive. According to the analysis, the crude fat and crude fiber contents of fresh grass of Humulus chinensis are 0.6% and 2.1% respectively. The dry matter of Humulus japonicus contains 12.8% to 20.46% of crude protein, about 2.03% of total flavonoids, 15.4% to 23.2% of crude fiber, 33.4% to 41.8% of nitrogen-free extract, 7.1% to ...

AI Technical Summary

Problems solved by technology

At present, the methods for extracting Humulus leaf protein and flavonoids are separated, and they are all for the purpose of extracting a single substance, which leads to repeated processing, time-consuming, inability to deeply utilize Humulus resources, and waste of Humulus resources.

There is no precedent for the simultaneous fractional extraction of leaf protein and total flavonoids from the fresh and tender stems and leaves of Humulus humulus, which are rich in protein (12.8%-20.46% dry matter) and total flavonoids (dry matter percentage content is about 2.03%)

Images