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Method for preparing porcine hepatitis E virus (HEV) total antibody enzyme-linked immuno sorbent assay (ELISA) detection kit

A technology for detection kits and total antibodies, which is applied in the field of preparation of ELISA detection kits for swine HEV total antibodies, can solve problems such as inability to detect hepatitis E virus, and achieve the effects of rapid and efficient detection, high sensitivity, and good prevention and control.

Active Publication Date: 2014-06-04
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology

[0008] The present invention is to provide a preparation method of a pig HEV total antibody ELISA detection kit, aiming at solving the problem that the existing hepatitis E virus kit can only specifically detect human hepatitis E virus, but cannot detect pig-derived hepatitis E virus The problem

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  • Method for preparing porcine hepatitis E virus (HEV) total antibody enzyme-linked immuno sorbent assay (ELISA) detection kit
  • Method for preparing porcine hepatitis E virus (HEV) total antibody enzyme-linked immuno sorbent assay (ELISA) detection kit
  • Method for preparing porcine hepatitis E virus (HEV) total antibody enzyme-linked immuno sorbent assay (ELISA) detection kit

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[0062] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0063] The application principle of the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0064] like figure 1 Shown, the preparation method of the pig HEV total antibody ELISA detection kit of the embodiment of the present invention comprises the following steps:

[0065] S101: preparing HEV coating antigen;

[0066] S102: Prepare HEV labeled antigen;

[0067] S103: Preparation of HEV marker antigen-HRP marker;

[0068] S104: Dilute the S antigen in a certain ratio with carbonate buffer solution, add 100 μl / well to the microplate plate, package i...

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Abstract

The invention discloses a method for preparing a porcine hepatitis E virus (HEV) total antibody enzyme-linked immuno sorbent assay (ELISA) detection kit. The method for preparing the porcine HEV total antibody ELISA detection kit comprises the following steps: preparing an HEV coating antigen; preparing an HEV labeled antigen; preparing an HEV labeled antigen-HRP marker; and diluting an S antigen according to a certain ratio by adopting a carbonate buffer solution, adding the diluent into an ELISA plate with the volume of 100mu l for each pore, packaging the ELISA plate in an aluminum coating bag filled with a drying agent, and finishing the coating. An ELISA detection method for rapidly and efficiently detecting porcine HEV total antibodies is established, the coating antigen and antibodies in serum are specifically bonded according to a dual antigen sandwich method principle, an antigen-antibody complex and the labeled antigen are bonded to carry out antigen-antibody reaction, the two antigens are bonded with different antigen-binding sites, the method is high in sensitivity, high in specificity and high in repeatability, a corresponding technical guarantee is provided for HEV epidemiology large-scale surveys, and a theoretical basis is provided for well preventing and controlling porcine HEV epidemic diseases.

Description

technical field [0001] The invention belongs to the detection technical field of hepatitis E virus, in particular to a preparation method of a porcine HEV total antibody ELISA detection kit. Background technique [0002] Hepatitis E (Hepatitis E) is a zoonotic infectious disease caused by Hepatitis E virus (HEV) transmitted through the digestive tract. The disease is prone to develop into fulminant hepatitis in adolescents, can lead to miscarriage in pregnant women, and the case fatality rate is as high as 21%. HEV is widely distributed and seriously harmful. [0003] There have been reports on pig-derived HEV from various countries in the world. The results of comparison with human HEV sequences revealed that the two have a high homology, and then it was found that the extracted RNA virus can infect chimpanzees and rhesus monkeys. Subsequently, successive experimental results showed that porcine hepatitis E is a zoonotic disease. Pigs are the main natural host of hepatiti...

Claims

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Application Information

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IPC IPC(8): G01N33/576C12N15/51C12N15/70C07K14/08
CPCG01N33/56983G01N33/5767
Inventor 兰喜杨彬常晓依柳纪省张韵殷相平李宝玉李学瑞李志勇方玉萍
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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