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Method for preparing biodiesel through trichosporon cutaneum

A technology of Trichosporium dermatosa and biodiesel, which is applied in the fields of bioenergy and biocatalysis, can solve the problems of increased production costs and the probability of infection with miscellaneous bacteria, and achieve the effects of easy control, high yield, and slow heating rate

Inactive Publication Date: 2014-06-11
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the problems in the prior art that require pre-saccharification treatment to increase the production cost and the probability of infection with miscellaneous bacteria, the present invention provides a method for preparing biodiesel by using Trichospora dermatosus yeast. In this method, Jerusalem artichoke is selected as oil production It can be used directly without saccharification of raw materials, realizing one-step fermentation of raw materials

Method used

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  • Method for preparing biodiesel through trichosporon cutaneum

Examples

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Effect test

Embodiment 1

[0026] A kind of method utilizing Trichosporium dermatosa yeast to prepare biodiesel, comprises the following steps:

[0027] 1) Preparation of inulin extract: Weigh 5kg of fresh Jerusalem artichoke, wash it with water, slice it with a slicer, spread it outdoors to dry, then put it in an oven, and dry it at 65°C until the water content is below 5%. After pulverizer pulverizes, cross 100 mesh sieves and make about 1.3kg Jerusalem artichoke powder. Weigh 100g of Jerusalem artichoke powder and dissolve it in 900mL of distilled water, place it in a constant temperature water bath, heat it in a water bath at 85°C for 80 minutes, centrifuge at 4000r / min for 10 minutes, remove the precipitate, and take the supernatant as the inulin extract;

[0028] 2) Preparation of lipid-producing medium: Enzymolyze the inulin extract prepared in step 1). Enzymolysis conditions: adjust the pH value of the inulin extract to 4.6 with 1 mmol / L HCl, add 3 mL of exo-inulinase enzyme solution with an en...

Embodiment 2

[0034] A kind of method utilizing Trichosporium dermatosa yeast to prepare biodiesel, comprises the following steps:

[0035] 1) The preparation of the inulin extract is the same as in Example 1;

[0036] 2) The preparation of lipid-producing medium is the same as that in Example 1;

[0037] 3) Trichospora dermatosus oil preparation, the same as in Example 1;

[0038] 4) Biodiesel preparation

[0039] Take 5g of the Trichospora dermatosus oil prepared in step 3) and place it in a one-necked flask, and irradiate it in a microwave oven for a certain period of time to remove moisture. Weigh 0.05g of NaOH required for the reaction and dissolve it in 30g of anhydrous methanol to prepare a catalyst methanol solution. The prepared catalyst methanol solution was poured into the flask, and then the flask was put into a microwave oven for heating. The microwave power was 400W, the heating time was 2.0min, and the reaction was carried out with stirring. After the reaction is finished...

Embodiment 3

[0042] A kind of method utilizing Trichosporium dermatosa yeast to prepare biodiesel, comprises the following steps:

[0043] 1) The preparation of the inulin extract is the same as in Example 1;

[0044] 2) The preparation of lipid-producing medium is the same as that in Example 1;

[0045] 3) Trichospora dermatosus oil preparation, the same as in Example 1;

[0046] 4) Biodiesel preparation

[0047] Take 5g of the Trichospora dermatosus oil prepared in step 3) and place it in a one-necked flask, and irradiate it in a microwave oven for a certain period of time to remove moisture. Weigh 0.05g of NaOH required for the reaction and dissolve it in 30g of anhydrous methanol to prepare a catalyst methanol solution. The prepared catalyst methanol solution was poured into the flask, and then the flask was put into a microwave oven for heating. The microwave power was 400W, the heating time was 3.0min, and the reaction was carried out with stirring. After the reaction was finishe...

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Abstract

The invention discloses a method for preparing biodiesel through trichosporon cutaneum. The method comprises the following steps: firstly, preparing microorganism grease, namely implementing batch fermentation culture under a certain culture condition by taking a synanthrin extracting solution as a grease producing culture medium and trichosporon cutaneum as a grease producing strain; drying and extracting to obtain microorganism grease; then, preparing biodiesel, namely heating the microorganism grease which serves as a raw material through microwave with presence of a catalyst, and carrying out a transesterification reaction with methanol to obtain the biodiesel. By producing the microorganism grease by virtue of fermentation of the jerusalem artichoke extracting solution, the method disclosed by the invention can be used for overcoming the defect of inulin produced grease which is high in production cost due to addition of a yeast extract, peptone and inorganic salt, obviously reducing the biodiesel production cost and realizing full use of the jerusalem artichoke resource; moreover, the established production process of the biodiesel through microwave heating is simple and convenient to operate, environment-friendly, quick in reaction speed, high in biodiesel yield and easy to control, and the production process has excellent industrial application prospect.

Description

technical field [0001] The invention relates to the technical fields of bioenergy and biocatalysis, in particular to a method for preparing biodiesel by using Trichosporium dermatosa yeast. Background technique [0002] Fossil energy has supported the progress of human civilization and economic and social development for nearly 200 years from the 19th century to the 20th century. However, due to the non-renewability of fossil energy and the huge consumption of it by humans, fossil energy is gradually becoming exhausted. At the same time, due to the large amount of fossil energy consumption, it also causes serious environmental pollution. Therefore, countries all over the world are seeking to replace fossil energy with renewable and clean energy. Among them, biodiesel is recognized as a green energy that can replace fossil fuels. [0003] Biodiesel refers to the long-chain fatty acid alkyl monoester obtained after esterification of animal, vegetable or microbial oil. Due t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/645
CPCY02E50/10
Inventor 纠敏汪伦记赵君峰黄狮李净净
Owner HENAN UNIV OF SCI & TECH
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