Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cytoplasmic male sterility restorer gene in rice and application thereof

A technology for male sterility and gene recovery, which is applied in the field of genetic engineering to achieve the effects of improving selection efficiency, speeding up the breeding process, and eliminating the need for test crosses.

Active Publication Date: 2014-06-18
SOUTH CHINA AGRI UNIV
View PDF1 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Rf3 and Rf4 Has been located in specific intervals of chromosome 1 and 10 (Zhang et al., 1997; Zhang Qunyu et al., 2002; Ahmadikhah and Karlov, 2006), but has not been isolated and cloned

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cytoplasmic male sterility restorer gene in rice and application thereof
  • Cytoplasmic male sterility restorer gene in rice and application thereof
  • Cytoplasmic male sterility restorer gene in rice and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Restoration gene in rice Rf4 positioning and cloning

[0045] The present invention is cloned by map-based cloning Rf4 Gene.

[0046] Firstly, the rice wild-abrupt cytoplasmic male sterile line Zhenshan 97A was crossed with the restorer line Minghui 63 to establish a F 2 Separate the population and select about 2000 sterile plants for Rf4 position. According to the reported preliminary location on chromosome 10 of rice Rf4 The approximate location of the site (Zhang et al., 1997; Zhang Qunyu et al., 2002; Ahmadikhah and Karlov, 2006), in which multiple polymorphic molecular markers as shown in Table 1 were created. with these marks and the F 2 Infertile populations were subjected to linkage analysis to construct a Rf4 The linkage map of locus regions, namely figure 1 . There are 2 marks in the picture namely M19391 and M19256 with Rf4 There is only 1 recombination event between loci, and 4 markers with Rf4 Complete co-segregation, ie zero re...

Embodiment 2

[0051] Example 2 Rf4 Transformation and functional testing of candidate genes

[0052] According to Os10g0495200 upstream and downstream sequence design gene-specific PCR amplification oligonucleotide primers, its primer sequence is as SEQ ID NO:11 (underline is restriction endonuclease Kpn I cut point) and SEQ ID NO:12 (underlined as Xba I cut point).

[0053] SEQ ID NO: 11: 5'-ACTTAT ggtacc TAGCAACTGCGAACACACCCTCAAT-3'

[0054] SEQ ID NO: 12: 5'-CGGTCA tctaga ACCAGCAACCTCCCAACCATGACTAT-3'

[0055] A 7.8 kb genomic fragment containing the promoter, coding region, and downstream termination sequence was amplified from the restorer line Minghui 63 by PCR. The PCR reaction system and reaction conditions are as follows: 1 x reaction buffer, 200 μM dNTPs, 100 ng Minghui 63 genomic DNA, 0.3 μM primers, 1.0 U KOD FX polymerase in a 50 μl reaction. 30 cycles: 97°C 15S, 68°C 6 min. restriction endonuclease Kpn I and Xba Clone into the plant binary transformation v...

Embodiment 3

[0057] Example 3 Rf4 gene sequence analysis

[0058] The functional types of five wild-type restorer lines Minghui 63, IR24, HNF-W1, HNF-W2, and HNF-W8 were analyzed by PCR amplification and sequencing Rf4 Allele ( Rf4-M, Rf4-I, Rf4-W1, Rf4-W2, Rf4-W8 ) and two non-functional CMS lines Zhenshan 97A and Jin 23A rf4 allele (rf4-Z, rf4-J ). Among them, HNF-W1, HNF-W2, HNF-W8 Rf4 allele ( Rf4-W1, Rf4-W2, Rf4-W8 ) is derived from common wild rice and obtained by crossing and backcrossing an indica rice line with a different common wild rice strain. The sequences shown in SEQ ID NO:1 and SEQ ID NO:2 are respectively Minghui 63 and IR24 Rf4-M and Rf4-I Genomic DNA fragments of alleles (including the sequence of the promoter region, CDS, and 3' untranslated region); the sequences shown in SEQ ID NO:3 to SEQ ID NO:5 are HNF-W1, HNF-W2, HNF-W8 Rf4-W1, Rf4-W2, Rf4-W8 Allele CDS sequence. These functional Rf4 The open reading frame of the allele has 2349 nucleotides,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of plant genetic engineering, and specifically discloses a cytoplasmic male sterility restorer gene in rice and an application thereof. The invention provides a nucleotide sequence of a wild abortive cytoplasmic male sterility restorer gene (i) Rf4 ( / i) isolated and cloned from rice and an amino acid polypeptide sequence encoded by the restorer gene. The gene belongs to a member of PPR (pentatricopeptide repeats) gene family and is a constitutively expressed gene. The fertility is restored by negatively regulating the expression levels of sterility gene (i) WA352 ( / i) in mitochondrial genome. A new restorer line can be cultivated by transforming (i) Rf4 ( / i) into the original rice varieties containing no restorer gene. Transgenic restorer lines are hybridized with a wild abortive type sterile line to produce hybrids having normal sterility for production.

Description

technical field [0001] The invention relates to the field of genetic engineering, and more specifically relates to rice cytoplasmic male sterility restoration gene and application thereof. Background technique [0002] Plants can produce hybrids through cross-pollination, and hybrids have advantages in growth vigor, adaptability, yield, etc., called heterosis. Harnessing heterosis in crops can improve yield, quality and resistance. The phenomenon that the male reproductive organs of plants cannot produce normal fertile male gametes, that is, pollen, is called male sterility. Crop varieties or lines with the genetic trait of male sterility are called sterile lines. On the other hand, hybrids produced by crossing some crop varieties (strains) with sterile lines have restored normal fertility, and these crop varieties (strains) are called restorer lines. By using the sterile line as the female parent and the restorer line as the male parent, the operation of detasseling can ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/29C12N15/82C12N5/10C12N15/11C07K14/415C12Q1/68A01H5/00
Inventor 刘耀光唐辉武罗荡平陈乐天张群宇
Owner SOUTH CHINA AGRI UNIV
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com