Injectable silk fibroin-alginate double cross-linked hydrogel and its preparation method and use method

A technology of alginate and hydrogel, applied in medical science, prosthesis, etc., can solve the problem of not being able to prepare injectable silk fibroin-alginate hydrogel, and achieve good biodegradability and gelation speed Fast and simple process effect

Inactive Publication Date: 2016-01-20
GUANGXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] Adopt above-mentioned method, all can not prepare injectable silk fibroin-alginate hydrogel

Method used

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  • Injectable silk fibroin-alginate double cross-linked hydrogel and its preparation method and use method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Solution A Preparation of silk fibroin solution: cut cocoons into pieces, add 0.05mol / LNa 2 CO 3 The aqueous solution, the liquor ratio is 1:200, heated to boiling, and kept for 60min to remove the sericin on the silk surface. After washing and drying, the silk fibroin was dissolved in CaCl 2 / EtOH / H 2 O (substance ratio: 1mol:2mol:8mol) dissolved in the system, placed in running water for 3 days to remove ethanol and free Ca in the solution 2+ , prepare 10 mL of silk fibroin solution with a mass concentration of 1 wt% with deionized water, stir and add 0.0451 g of CaCl 2 .

[0040] Preparation of solution B modified alginate solution: dissolve alginate, prepare 10 mL of alginate solution with a mass concentration of 2wt% with deionized water, add 1-ethyl-3, a crosslinking agent with activating properties to carboxyl groups -(3-Dimethylaminopropyl) carbodiimide (EDC) 0.0052 g, N-hydroxysuccinimide (NHS) 0.0016 g, pH 7.0 of the solution, stirring, and aging for 100 ...

Embodiment 2

[0043] Preparation of solution A: prepare 10 mL of silk fibroin solution with a mass concentration of 1 wt % according to the method described in Example 1, stir and add 0.0618 g of CaCl 2 .

[0044] Preparation of solution B: prepare according to the procedure described in Example 1.

[0045] Cross-linking: According to the steps described in Example 1, a double-cross-linked hydrogel with covalently cross-linked amino groups and carboxyl groups and cross-linked by metal ions was prepared. As the concentration of calcium ions increases, the gelation time of the obtained hydrogel is reduced to 100s; the cell size of the hydrogel is reduced, and the cell distribution is uniform; the elastic modulus is increased to 700~900Pa, and the swelling rate of water absorption is reduced , the degradation rate is reduced.

Embodiment 3

[0047] Preparation of solution A: follow the procedure described in Example 1.

[0048] Preparation of solution B: prepare 10 mL of alginate solution with a mass concentration of 2 wt % as described in Example 1, and add 1-ethyl-3-(3-dimethylaminopropyl), a cross-linking agent with activating properties to carboxyl groups. ) carbodiimide (EDC) 0.0104 g, N-hydroxysuccinimide (NHS) 0.0031 g, the solution pH 7.0, stirring, and aging for 100 min.

[0049] Crosslinking: carried out as described in Example 1.

[0050] Increasing the concentration of the carboxyl-activated crosslinking agent increases the gelation time of the obtained hydrogel to 240 s, the elastic modulus increases, the water absorption swelling rate of the hydrogel decreases, and the degradation rate decreases.

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Abstract

The invention relates to injectable silk fibroin-alginate double cross-linking hydrogel which comprises a solution A: a silk fibroin solution and a solution B: a modified alginate solution, wherein the solution A comprises the following components in parts by weight: 1.0*10<-1>-3.0 parts of silk fibroin, 1.4*10<-3>-2.0*10<-3> part of a divalent metal ion cross-linking agent, and the balance of water containing 1-5wt% of silk fibroin; the solution B comprises the following components in parts by weight: 5.0*10<-1>-4.0 parts of alginate, 2.5*10<-3>-4.0*10<-1> part of a carboxyl activating cross-linking agent and the balance of water containing 1-5wt% of alginate. By adopting a double cross-linking method of covalent cross-linking and ion cross-linking, the double cross-linking hydrogel is quick to polymerize in situ and has an application prospect in the fields such as engineering stents, drug controlled release and regenerative medicines. The invention further provides a preparation method and a use method of the double cross-linking hydrogel.

Description

technical field [0001] The invention belongs to biomedical materials, in particular relates to a processing and utilization technology of silk fibroin, more particularly an injectable silk fibroin-alginate double cross-linked hydrogel and a preparation method and use method thereof. Background technique [0002] Loss and dysfunction of tissues and organs are one of the main reasons that affect the quality of human life and cause human disease and death. The repair and reconstruction of damaged organs is a major problem faced by biology and clinical medicine. Tissue engineering utilizes scaffold materials, seed cells, and growth factors to regenerate tissues and organs in vitro in bioreactors or in vivo. Scaffolding materials for tissue repair are one of the central contents of tissue engineering and regenerative medicine. In terms of physical properties, it is the space for seed cells to attach and survive, providing spatial architecture, mechanical requirements and geomet...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22A61L27/20A61L27/52C08J3/075C08J3/24
Inventor 沈星灿李子恒纪仕辰王亚珍覃山福
Owner GUANGXI NORMAL UNIV
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