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Color detection device and method of portable paper-base micro fluidic chip

A technology of microfluidic chips and detection devices, which is applied in the direction of analyzing materials through chemical reactions and observing the influence of chemical indicators on materials, etc., which can solve the problems affecting detection accuracy and stability, data conversion processing The process is cumbersome and other problems, to achieve the effect of extremely low reagent consumption, low price and low reagent consumption

Inactive Publication Date: 2014-07-02
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has obvious disadvantages. The data conversion process is cumbersome. In the home-based self-diagnosis, the person being examined needs to have good professional knowledge.
What's more, the light in the environment will affect the accuracy and stability of the detection

Method used

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  • Color detection device and method of portable paper-base micro fluidic chip
  • Color detection device and method of portable paper-base micro fluidic chip
  • Color detection device and method of portable paper-base micro fluidic chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Fabrication of a microfluidic chip

[0036] The paper microfluidic chip is to use the paper chip (filter paper, nitrocellulose paper or other materials) to generate hydrophobic and hydrophilic regions by spraying wax on the printer or other methods to realize the pattern design of the chip, and fix the display in the reaction area in advance. Color agent or biochemical reagent, when the sample is dropped, the area will produce color, the depth of the color is related to the concentration of the substance to be tested, the paper chip is inserted from the entrance of the card slot, and the color detection sensor equipped with it can detect the paper chip The reflected light of the reaction area is detected, and the qualitative and quantitative detection of the target substance can be carried out conveniently, quickly, stably and effectively.

Embodiment 2

[0037] Example 2: Research on real-time quantitative detection of protein (BSA) by the device

[0038] Add 3 μL of 0.3M citrate buffer (pH=1.8) to the reaction area of ​​the microfluidic paper chip described in Example 1, and then add 3 μL of bromophenol blue solution on top, and wait for the above After the solution was dried in air, 3 μL of bovine serum albumin (BSA) solution to be tested was added to the center of the sample. Insert the paper chip into the portable (handheld) paper chip color detection device, and read and record the measured value after 2 minutes of reaction. Its quantitative results are as figure 2 As shown, bovine serum albumin (BSA) can be accurately quantified in the range of 5-50 μM. The method utilizes that the analyte with different concentrations on the chip can undergo a chemical or biological color reaction under the action of its corresponding chromogenic reagent, and the depth of the color reaction is linearly related to the concentration of...

Embodiment 3

[0039] Embodiment 3: This device takes glucose (glucose) as the real-time quantitative detection research of object

[0040] Add 3 μL of potassium iodide solution (0.6 M) dropwise to the reaction area of ​​the microfluidic paper chip described in Example 1, and then add 3 μL of a solution of horseradish peroxidase and glucose oxidase dropwise on it, After the solution is dry, add 3 μL of glucose (glucose) dropwise on it, insert the paper chip into a portable (handheld) paper chip color detection device, and read and record the measured value after 2 minutes of reaction. Its quantitative results are as image 3 As shown, glucose can be accurately quantified in the range of 10-100 μM.

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Abstract

The invention relates to a color detection device and method of a portable paper-base micro fluidic chip; the paper-base chip is placed in a chip card slot, the chip card slot is arranged in the device; a LED light source emitted by a light emitter irradiates a reaction region of the paper-base chip to reflect out reflected light; a color detection sensor is connected with a signal amplifying unit, is used for receiving the reflected light, and converts a light signal into an electrical signal to sent into the digital amplifier unit for signal amplification processing; a digital-to-analog conversion unit is connected with the signal amplifying unit and a programmable controller, is used for receiving an amplified digital electrical signal, and converts the digital electrical signal into an analog electrical signal to sent into the programmable controller for data processing. The color detection device is a new microfluidic device designed on the basis of the principle of light reflection on paper, is used for colorimetric analysis of biochemical reaction related color to provide quantitative analysis data, and can be widely used in the fields of family disease rapid diagnosis, on-site instant detection and the like.

Description

technical field [0001] The invention relates to a paper chip preparation technology, in particular to a portable paper-based microfluidic chip color detection device and method. Background technique [0002] Microfluidic paper chip technology is currently a research hotspot in instrumental analysis. This technology is mainly based on analytical chemistry and biochemistry and is used in many fields such as chemical analysis, environmental monitoring, immune analysis, disease diagnosis, and cell analysis. As a key technology in the microfluidic chip platform, it has received a lot of attention from researchers in this field. [0003] Currently, health care workers lack adequate equipment to diagnose common diseases. Therefore, in resource-constrained settings, the development of low-cost diagnostic technologies has shown the potential and status of portable and suitable equipment for home medical applications. These devices are small, inexpensive, and don't require a lot of ...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 李博伟陈令新付龙文
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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