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Method and device for mutagenizing biomaterial

A biomaterial and chemical mutagen technology, applied in the field of high-throughput treatment of biomaterials, can solve the problems of reduced treatment effect, weakened liquid treatment dose, low mutagenesis efficiency, etc. Screening efficiency, the effect of improving mutagenesis efficiency

Inactive Publication Date: 2014-07-09
WUXI TMAXTREE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The disadvantage of the above-mentioned existing method is that, when the microorganisms in the liquid state contained in the container are subjected to mutagenesis treatment, especially when they are treated by physical methods, due to mutual shielding between the bacteria, or the weakening effect of the liquid on the treatment dose, Will reduce the treatment effect, resulting in low mutagenesis efficiency
[0004] The above processing methods are also not conducive to high-throughput and automated operations

Method used

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  • Method and device for mutagenizing biomaterial
  • Method and device for mutagenizing biomaterial

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Embodiment 1 adopts method mutagenesis of the present invention to treat lactic acid bacteria

[0032] Equipment: ARTP (atmospheric room temperature plasma) mutagenesis breeding system

[0033] Reagent: 0.9% normal saline, MRS solid medium formula: peptone 10g / L, beef powder 5g / L, yeast powder 4g / L, glucose 20g / L, sodium acetate 5g / L, diammonium citrate 2g / L, spit Warm 801mL, dipotassium hydrogen phosphate (7H 2 O) 2g / L, sodium acetate (3H 2 O) 5g / L, triammonium citrate 2g / L, magnesium sulfate (7H 2 O) 0.2g / L, manganese sulfate (4H 2 O) 0.05g / L, agar 15g / L.

[0034] Bacteria liquid to be treated: Lactobacillus acidophilus ACCC10637, from China Agricultural Microorganism Culture Collection Management Center

[0035] Solid medium: MRS solid medium

[0036] Control test steps:

[0037] (1) Preparation of bacterial suspension: the original strain of lactic acid bacteria was activated and cultured with MRS solid medium, the culture temperature was 35°C, and the cultur...

Embodiment 2

[0049] Embodiment 2 adopts method mutagenesis treatment yeast of the present invention

[0050] Equipment: ARTP (atmospheric room temperature plasma) mutagenesis breeding system

[0051] Reagent: 0.9% normal saline, PDA solid medium formula: potato powder 5g / L, glucose 20g / L, agar 20g / L, chloramphenicol 0.1g / L.

[0052] Bacteria to be treated: Pichia pastoris 21003, purchased from China Agricultural Microorganism Culture Collection Management Center

[0053] Solid medium: PDA solid medium

[0054] Control test steps:

[0055] (1) Preparation of bacterial suspension: the original strain of yeast was activated and cultured with PDA solid medium, the culture temperature was 28°C, and the culture time was 48 hours, and the bacteria were scraped and placed in normal saline to obtain vigorous growth and thick bacteria liquid;

[0056] (2) Preparation of sample slides: Take freshly prepared bacterial solution and dilute to cell concentration OD 600 =0.5~1, drop 10~20uL onto the ste...

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Abstract

The invention discloses a method and a device for mutagenizing a biomaterial and belongs to the field of biotechnology. The method comprises the following steps of 1, coating a solid medium with cells to be treated, and 2, mutagenizing the coating cells which comprise microbial cells and suspension cells of animals and plants. The method can substantially improve mutagenesis efficiency and screening efficiency and is conducive to mutagenesis breeding adopting automatic high-flux experimental equipment.

Description

technical field [0001] The invention relates to a biological test method, in particular to a method and device for high-throughput processing of biological materials. Background technique [0002] At present, most laboratories use mutagenesis treatment methods for biological materials, especially microorganisms, and the treatment process is mostly as follows: (1) Mutagenesis treatment: through physical or chemical The sample is subjected to mutagenesis treatment; (2) Coating plate: After the mutagenesis treatment is completed, spread the bacterial solution on the solid medium to grow a single colony; (3) Analyze the single colony to screen mutants. [0003] The disadvantage of the above-mentioned existing method is that, when the microorganisms in the liquid state contained in the container are subjected to mutagenesis treatment, especially when they are treated by physical methods, due to mutual shielding between the bacteria, or the weakening effect of the liquid on the tr...

Claims

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Application Information

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IPC IPC(8): C12N15/01C12N13/00C12R1/23C12R1/84
Inventor 毕鲜荣王立言
Owner WUXI TMAXTREE BIOTECHNOLOGY CO LTD
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