Bone repair material prepared by combining silk fibroin and decalcified bone matrix

A technology of decalcified bone matrix and silk fibroin, which is applied in the field of bone repair materials, can solve problems such as poor shape maintenance, increased bleeding volume, low water solubility, etc., and achieve good osteoinductive and osteoconductive capabilities and good shape Retention and implantability, effects in repairing bone defects and nonunions

Inactive Publication Date: 2014-07-16
BEIHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Autologous bone contains osteoinductive factors, osteoconductive matrix and osteogenic cells, which can induce pluripotent stem cells to differentiate into osteoblasts, which is beneficial to the growth of blood vessels and bone regeneration. Therefore, autologous bone is an ideal bone graft material for humans, but its It has the following disadvantages: 1. The source is limited, especially it cannot meet the needs of large-segment bone defect surgery; 2. The patient needs to bear the extra pain caused by the complications of the donor site; 3. The operation time is prolonged, and the amount of bleeding is correspondingly increased
Due to the poor mechanical properties of DBM, it is not suitable for use in parts that need to bear gravity and other stresses, which greatly reduces the scope of use of DBM
In addition, due to the relatively low water solubility of DBM, when DBM is used alone for transplantation, it cannot maintain the desired shape well, and the bone growth protein contained in DBM may also be digested and hydrolyzed in vivo
In addition, due to the low implantability of DBM due to the incomplete shape retention ability of DBM, finding a material with good biocompatibility and good mechanical properties to compound with DBM has become one of the effective methods to solve the problem.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation method of the bone repair material composited with silk fibroin and decalcified bone matrix is ​​as follows: it is a bone repair material composed of the following weight ratio, decalcified bone matrix: silk fibroin=1:5. And the concentration of the silk fibroin solution is 17% (g / ml).

[0027] a) Preparation of silk fibroin:

[0028] Bombyx mori silk was placed in 0.1% Na 2 CO 3 In the solution, treat at 98-100°C for 30 minutes, repeat three times, to remove sericin on the surface of silk fiber, and dry in air at room temperature. Put the silk fiber into CaCl 2 -CH 3 CH 2 OH-H 2 O (1:2:8 molar ratio) solution was dissolved, and the temperature was kept at 78±2°C with constant stirring to obtain a silk fibroin solution, which was then dialyzed in distilled water with a dialysis tube. Finally, the silk fibroin solution is freeze-dried;

[0029] b) Preparation of blended solution:

[0030] Put 0.85 g of freeze-dried silk fibroin and 0.17 g of deca...

Embodiment 2

[0038] The preparation method of the bone repair material composited with silk fibroin and decalcified bone matrix is ​​as follows: it is a bone repair material composed of the following weight ratio, decalcified bone matrix: silk fibroin = 2:5. And the concentration of the silk fibroin solution is 17% (g / ml).

[0039] a) Preparation of silk fibroin:

[0040] Bombyx mori silk was placed in 0.1% Na 2 CO 3 In the solution, treat at 98-100°C for 30 minutes, repeat three times, to remove sericin on the surface of silk fiber, and dry in air at room temperature. Put the silk fiber into CaCl 2 -CH 3 CH 2 OH-H 2 O (1:2:8 molar ratio) solution was dissolved, and the temperature was kept at 78±2°C with constant stirring to obtain a silk fibroin solution, which was then dialyzed in distilled water with a dialysis tube. Finally, the silk fibroin solution is freeze-dried in a freeze dryer;

[0041] b) Preparation of blended solution:

[0042] Put 0.85 g of freeze-dried silk fibroi...

Embodiment 3

[0050] The preparation method of the bone repair material composited with silk fibroin and decalcified bone matrix is ​​as follows: it is a bone repair material composed of the following weight ratio, decalcified bone matrix: silk fibroin = 4:5. And the concentration of the silk fibroin solution is 17% (g / ml).

[0051] a) Preparation of silk fibroin:

[0052] Bombyx mori silk was placed in 0.1% Na 2 CO 3 In the solution, treat at 98-100°C for 30 minutes, repeat three times, to remove sericin on the surface of silk fiber, and dry in air at room temperature. Put the silk fiber into CaCl 2 -CH 3 CH 2 OH-H 2 O (1:2:8 molar ratio) solution was dissolved, and the temperature was kept at 78±2°C with constant stirring to obtain a silk fibroin solution, which was then dialyzed in distilled water with a dialysis tube. Finally, the silk fibroin solution is freeze-dried in a freeze dryer;

[0053] b) Preparation of blended solution:

[0054] Put 0.85g of freeze-dried silk fibroin...

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PUM

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Abstract

The invention relates to the technical field of biomaterials, and relates to a preparation method for a bone repair material. The bond repair material is composed of silk fibroin and decalcified bone matrix. A salting-out process is employed for preparing the bone repair material by combining silk fibroin and decalcified bone matrix. The preparation method helps to keep the bone induction and bone conduction capabilities of decalcified bone matrix, and helps to improve the mechanic properties of the bone repair material. The prepared bone repair material has good promotion capability on bone regeneration and excellent mechanical properties, and is substantially improved in shape keeping capability and implanting capability.

Description

(1) Technical field [0001] The invention relates to the field of biomedical materials, in particular to a bone repair material composited with silk fibroin and decalcified bone matrix, which has good osteoinductive and osteoconductive effects and excellent mechanical properties. (2) Background technology [0002] As we all know, bone defects and nonunions have always been a major problem faced by orthopedic surgeons, and how to repair bone defects and nonunions has always been a thorny issue in the orthopedic field. In recent years, bone graft substitutes have become a research hotspot. Currently, the commonly used repair materials include autologous bone, allogeneic bone and synthetic substitutes. Autologous bone contains osteoinductive factors, osteoconductive matrix and osteogenic cells, which can induce pluripotent stem cells to differentiate into osteoblasts, which is beneficial to the growth of blood vessels and bone regeneration. Therefore, autologous bone is an idea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/22
Inventor 刘海峰樊瑜波丁希丽
Owner BEIHANG UNIV
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