A kind of extraction method of triticale bran proanthocyanidins
A bran proanthocyanidin and extraction method technology, which is applied in the field of extraction of black wheat bran proanthocyanidin, can solve the problem of no flavonoid anthocyanin-proanthocyanidin extraction and enrichment, etc., to achieve improved utilization value, easy operation and processing, and convenient industrial production Effect
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Embodiment 1
[0025] A method for extracting triticale bran proanthocyanidins, the specific steps of the method are:
[0026] (1) Pretreatment: remove impurities and pulverize the black wheat bran to 80 mesh to obtain black wheat bran powder, set aside;
[0027] (2) Degreasing: Take 50g of black wheat bran powder in step (1), soak in 450g of petroleum ether for 24 hours, filter, recover petroleum ether from the filtrate, dry the filter residue at room temperature or low temperature to remove residual petroleum ether, and obtain defatted black wheat bran powder. spare;
[0028] (3) Extraction: a. According to the solid-to-liquid ratio of defatted triticale bran powder and ethanol solution with a concentration of 60% by volume and a pH of 3: 1:9, mix and soak for 10 minutes, microwave power is 300w to assist extraction, and the extraction time is 20s to obtain mixture a; b. Stir and extract mixture a at a stirring speed of 100r / min at a temperature of 50°C, and extract for 80 minutes t...
Embodiment 2
[0031] A method for extracting triticale bran proanthocyanidins, the specific steps of the method are:
[0032] (1) Pretreatment: remove impurities and pulverize the black wheat bran to 80 mesh to obtain black wheat bran powder, set aside;
[0033] (2) Degreasing: Take 50g of black wheat bran powder in step (1), soak in 450g of petroleum ether for 24 hours, filter, recover petroleum ether from the filtrate, dry the filter residue at room temperature or low temperature to remove residual petroleum ether, and obtain defatted black wheat bran powder. spare;
[0034] (3) Extraction: a. According to the solid-to-liquid ratio of defatted black wheat bran powder and ethanol solution with a concentration of 50% by volume and a pH value of 4: 1:11, mix and soak for 10 minutes, microwave power is 300w to assist extraction, and the extraction time is 20s to obtain mixture a; b. Stir and extract mixture a at a stirring speed of 100r / min at a temperature of 80°C, and extract for 80 mi...
Embodiment 3
[0037] A method for extracting triticale bran proanthocyanidins, the specific steps of the method are:
[0038] (1) Pretreatment: remove impurities and pulverize the black wheat bran to 80 mesh to obtain black wheat bran powder, set aside;
[0039] (2) Degreasing: Take 50g of black wheat bran powder in step (1), soak in 450g of petroleum ether for 24 hours, filter, recover petroleum ether from the filtrate, dry the filter residue at room temperature or low temperature to remove residual petroleum ether, and obtain defatted black wheat bran powder. spare;
[0040] (3) Extraction: a. According to the solid-to-liquid ratio of defatted black wheat bran powder and ethanol solution with a concentration of 50% by volume and a pH value of 4: 1:11, mix and soak for 10 minutes, microwave power is 300w to assist extraction, and the extraction time is 20s to obtain mixture a; b. Stir and extract mixture a at a stirring speed of 100r / min at a temperature of 60°C for 40 minutes to obta...
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