Tissue-culture rapid seedling growing method for apple rootstock M9

A rootstock and apple technology, applied in the field of plant tissue culture, can solve the problems of difficulty in large-scale factory production, low propagation coefficient of seedlings, and low survival rate of transplanting, so as to achieve neat root system, good rooting effect, and survival after cultivation. high rate effect

Inactive Publication Date: 2014-07-23
天水市果树研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, the multiplication coefficient of apple rootstock M9 tissue culture seedlings is low, rooting i

Method used

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  • Tissue-culture rapid seedling growing method for apple rootstock M9
  • Tissue-culture rapid seedling growing method for apple rootstock M9
  • Tissue-culture rapid seedling growing method for apple rootstock M9

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) establishment of sterile system

[0031] On the morning of continuous sunny days from April to May, cut the stem segments of 3-4 nodes at the top of the new shoots of apple rootstock M9, remove the large leaves, cut 2-3cm long, put them into jars, and bring them back to the laboratory for later use. Soak the harvested stems in 1000 times dilution of detergent for 30 minutes, rinse them with running water for 2 hours, soak them with 75% alcohol for 10 seconds under aseptic conditions, rinse them with sterile water 5 times, Depending on the tenderness of the material, soak in 0.1% mercuric chloride solution for 5 minutes, then rinse with sterile water for 5 times, then place the surface-sterilized material on sterile filter paper, absorb excess water on the material, and cut off the injured medicine. On the surface, cut into a single bud stem section about 1.5-2cm in length, and insert it into the initiation differentiation medium as an explant. The starting differen...

Embodiment 2

[0049] In this embodiment, the initiation of differentiation medium is prepared by adding the following raw materials and their proportions to 1 liter of MS basic medium:

[0050]

[0051] In this embodiment, the expansion subculture medium is prepared by adding the following raw materials and their proportions to 1 liter of MS basic medium:

[0052]

[0053] In the present embodiment, the rooting medium is made by adding the following raw materials and their proportions in 1 liter of 1 / 3MS basic medium:

[0054] White sugar 25g

[0055] Agar powder 5g

[0056] Indolebutyric acid 0.12mg

[0057] In this embodiment, other steps are the same as in Embodiment 1.

Embodiment 3

[0059] In this embodiment, the initiation of differentiation medium is prepared by adding the following raw materials and their proportions to 1 liter of MS basic medium:

[0060]

[0061] In this embodiment, the expansion subculture medium is prepared by adding the following raw materials and their proportions to 1 liter of MS basic medium:

[0062]

[0063]

[0064] In the present embodiment, the rooting medium is made by adding the following raw materials and their proportions in 1 liter of 1 / 3MS basic medium:

[0065] White sugar 25g

[0066] Agar powder 5g

[0067] Indolebutyric acid 0.1mg

[0068] In this embodiment, other steps are the same as in Embodiment 1.

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Abstract

A disclosed tissue-culture rapid seedling growing method for apple rootstock M9 comprises the following steps: (1) establishing a sterile system, specifically selecting an apple rootstock M9 single-plant nutrition branch, scissoring to obtain a stem segment with terminal buds, inoculating to an induction differentiation medium for culture, wherein the medium is prepared by adding the following raw materials into 1 L of an MS basic medium: 25 g of white sugar, 5 g of agar powder, 0.2-1.0 mg of benzyladenine and 0.2-0.6 mg of naphthylacetic acid; (2) performing propagation subculturing, specifically putting in a propagation subculturing medium for culture, wherein the medium is prepared by adding the following raw materials into 1 L of an MS basic medium: 25 g of white sugar, 5 g of agar powder, 0.2-1.0 mg of benzyladenine, 0.2-0.6 mg of naphthylacetic acid and 0.2-0.5 g of hydrolyzed casein; (3) performing rooting culture, specifically putting in a rooting medium for rooting culture, wherein the medium is prepared by adding the following raw materials into 1 L of an 1/3 MS basic medium: 25 g of white sugar, 5 g of agar powder and 0.05-0.12 mg of indolebutyric acid; and (4) performing hardening culture on seedlings and transplanting.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a method for propagating apple rootstock M9 through plant tissue culture technology. Background technique [0002] Apple dwarf cultivation is the development trend of apple cultivation in the world. Therefore, when developing dwarf and dense apple cultivation, it is very important to select dwarf stock varieties according to local conditions. The advantage of apple rootstock M9 is that the tree body is short, suitable for dense planting, early fruiting, and quick production. The yield is high, the fruit quality is good, the management is convenient, and the production cost is reduced. [0003] The method of plant tissue culture is to use the totipotency of plant cells, that is, every cell that makes up a plant has the potential to develop into a complete plant, and take a single cell, cell mass, meristematic tissue or part of an organ on a plant, and artificially Th...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王艳芳李海青杨映红杨瑞斌王花李帼英杨俊霞郭志刚赵新红周代琴马建芳
Owner 天水市果树研究所
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